Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes

Anukanth Anumanthan, Armand Bensussan, Laurence Boumsell, Andreas D. Christ, Richard S. Blumberg, Stephan D. Voss, Amish T. Patel, Michael Robertson, Lee M. Nadler, Gordon J. Freeman

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Abstract

Expression of the BY55 protein has been shown to be tightly associated with NK and CD8+ T lymphocytes with cytolytic effector activity. To determine the function of this protein, we molecularly cloned BY55 cDNA. The cDNA sequence predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to killer inhibitory receptors. Reduction and carboxyamidomethylation of immunoprecipitated BY55 gave a band of 27 kDa, whereas reduction alone led to an 80-kDa species, suggesting that BY55 is a tightly disulfide-linked multimer. RNA blot analysis revealed BY55 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to NK and T cells. BY55 was expressed on the CD56(dim), CD16+ subset of NK cells, which have high cytolytic activity, but was not expressed and was not induced on the CD56(bright), CD16+ subset of NK cells, a subset with high proliferative, but low cytolytic, capacity. In human tissues, BY55 mRNA was expressed only in spleen, PBL, and small intestine (in gut lymphocytes). BY55 was expressed on all intestinal intraepithelial lymphocytes, which were predominantly CD3+TCRα/β+CD4- CD8+CD11b+CD28-CD45RO+CD56-CD101+CD103+ (α(E)β7 integrin). In addition, BY55 was expressed on most CD8+CD28- peripheral blood T cells. These phenotypic relationships suggest that CD8+CD28+ precursor CTL may terminally differentiate into CD8+CD28-BY55+ effector CTL and that some of the peripheral blood CD8+CD28- subset may represent recirculation from mucosal epithelial immune sites.

Original languageEnglish (US)
Pages (from-to)2780-2790
Number of pages11
JournalJournal of Immunology
Volume161
Issue number6
StatePublished - Sep 15 1998
Externally publishedYes

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Natural Killer Cells
Organism Cloning
Lymphocytes
T-Lymphocytes
Complementary DNA
KIR Receptors
Glycosylphosphatidylinositols
Messenger RNA
Proteins
Integrins
Disulfides
Small Intestine
Cysteine
Blood Cells
Spleen
RNA
Amino Acids
Immunoglobulin Domains

ASJC Scopus subject areas

  • Immunology

Cite this

Anumanthan, A., Bensussan, A., Boumsell, L., Christ, A. D., Blumberg, R. S., Voss, S. D., ... Freeman, G. J. (1998). Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. Journal of Immunology, 161(6), 2780-2790.

Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. / Anumanthan, Anukanth; Bensussan, Armand; Boumsell, Laurence; Christ, Andreas D.; Blumberg, Richard S.; Voss, Stephan D.; Patel, Amish T.; Robertson, Michael; Nadler, Lee M.; Freeman, Gordon J.

In: Journal of Immunology, Vol. 161, No. 6, 15.09.1998, p. 2780-2790.

Research output: Contribution to journalArticle

Anumanthan, A, Bensussan, A, Boumsell, L, Christ, AD, Blumberg, RS, Voss, SD, Patel, AT, Robertson, M, Nadler, LM & Freeman, GJ 1998, 'Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes', Journal of Immunology, vol. 161, no. 6, pp. 2780-2790.
Anumanthan A, Bensussan A, Boumsell L, Christ AD, Blumberg RS, Voss SD et al. Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. Journal of Immunology. 1998 Sep 15;161(6):2780-2790.
Anumanthan, Anukanth ; Bensussan, Armand ; Boumsell, Laurence ; Christ, Andreas D. ; Blumberg, Richard S. ; Voss, Stephan D. ; Patel, Amish T. ; Robertson, Michael ; Nadler, Lee M. ; Freeman, Gordon J. / Cloning of BY55, a novel Ig superfamily member expressed on NK cells, CTL, and intestinal intraepithelial lymphocytes. In: Journal of Immunology. 1998 ; Vol. 161, No. 6. pp. 2780-2790.
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abstract = "Expression of the BY55 protein has been shown to be tightly associated with NK and CD8+ T lymphocytes with cytolytic effector activity. To determine the function of this protein, we molecularly cloned BY55 cDNA. The cDNA sequence predicts a cysteine-rich, glycosylphosphatidylinositol-anchored protein of 181 amino acids with a single Ig-like domain weakly homologous to killer inhibitory receptors. Reduction and carboxyamidomethylation of immunoprecipitated BY55 gave a band of 27 kDa, whereas reduction alone led to an 80-kDa species, suggesting that BY55 is a tightly disulfide-linked multimer. RNA blot analysis revealed BY55 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to NK and T cells. BY55 was expressed on the CD56(dim), CD16+ subset of NK cells, which have high cytolytic activity, but was not expressed and was not induced on the CD56(bright), CD16+ subset of NK cells, a subset with high proliferative, but low cytolytic, capacity. In human tissues, BY55 mRNA was expressed only in spleen, PBL, and small intestine (in gut lymphocytes). BY55 was expressed on all intestinal intraepithelial lymphocytes, which were predominantly CD3+TCRα/β+CD4- CD8+CD11b+CD28-CD45RO+CD56-CD101+CD103+ (α(E)β7 integrin). In addition, BY55 was expressed on most CD8+CD28- peripheral blood T cells. These phenotypic relationships suggest that CD8+CD28+ precursor CTL may terminally differentiate into CD8+CD28-BY55+ effector CTL and that some of the peripheral blood CD8+CD28- subset may represent recirculation from mucosal epithelial immune sites.",
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AU - Bensussan, Armand

AU - Boumsell, Laurence

AU - Christ, Andreas D.

AU - Blumberg, Richard S.

AU - Voss, Stephan D.

AU - Patel, Amish T.

AU - Robertson, Michael

AU - Nadler, Lee M.

AU - Freeman, Gordon J.

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