Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor

P. Lobel, D. M. Dahms, J. Breitmeyer, John Chirgwin, S. Kornfeld

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

Four overlapping cDNA clones encoding a partial sequence of the cation-independent 215-kDa mannose 6-phosphate receptor have been identified by screening a fetal calf liver cDNA library with oligonucleotide probes. RNA hybridization analysis showed that the length of the mRNA is ~ 9.5 kilobases. Sequence analysis demonstrated that the clones consist of 4647 contiguous nucleotides and contain an open reading frame coding for a polypeptide of 1461 amino acids, which we estimate represents > 75% of the primary structure of the receptor. The deduced amino acid sequence indicates that the receptor has a carboxyl-terminal cytoplasmic domain of 163 amino acids that is rich in acidic residues, a 23-amino acid transmembrane segment, and an extracellular domain containing at least eight homologous repeats of ~ 145 amino acids. One of the repeats contains an additional 43-residue segment that is similar to the type II repeat of fibronectin. Each repeat contains a highly conserved 13-amino acid unit bordered by cysteine residues that may be functionally important.

Original languageEnglish (US)
Pages (from-to)2233-2237
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number8
DOIs
StatePublished - 1987
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
Cations
Organism Cloning
Amino Acids
Clone Cells
Oligonucleotide Probes
Gene Library
Fibronectins
Open Reading Frames
Cysteine
Sequence Analysis
Amino Acid Sequence
Nucleotides
Complementary DNA
RNA
Messenger RNA
Peptides
Liver

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor. / Lobel, P.; Dahms, D. M.; Breitmeyer, J.; Chirgwin, John; Kornfeld, S.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 8, 1987, p. 2233-2237.

Research output: Contribution to journalArticle

@article{02d23198ed9c4b7b9690a3ed85b2d8e0,
title = "Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor",
abstract = "Four overlapping cDNA clones encoding a partial sequence of the cation-independent 215-kDa mannose 6-phosphate receptor have been identified by screening a fetal calf liver cDNA library with oligonucleotide probes. RNA hybridization analysis showed that the length of the mRNA is ~ 9.5 kilobases. Sequence analysis demonstrated that the clones consist of 4647 contiguous nucleotides and contain an open reading frame coding for a polypeptide of 1461 amino acids, which we estimate represents > 75{\%} of the primary structure of the receptor. The deduced amino acid sequence indicates that the receptor has a carboxyl-terminal cytoplasmic domain of 163 amino acids that is rich in acidic residues, a 23-amino acid transmembrane segment, and an extracellular domain containing at least eight homologous repeats of ~ 145 amino acids. One of the repeats contains an additional 43-residue segment that is similar to the type II repeat of fibronectin. Each repeat contains a highly conserved 13-amino acid unit bordered by cysteine residues that may be functionally important.",
author = "P. Lobel and Dahms, {D. M.} and J. Breitmeyer and John Chirgwin and S. Kornfeld",
year = "1987",
doi = "10.1073/pnas.84.8.2233",
language = "English (US)",
volume = "84",
pages = "2233--2237",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "8",

}

TY - JOUR

T1 - Cloning of the bovine 215-kDa cation-independent mannose 6-phosphate receptor

AU - Lobel, P.

AU - Dahms, D. M.

AU - Breitmeyer, J.

AU - Chirgwin, John

AU - Kornfeld, S.

PY - 1987

Y1 - 1987

N2 - Four overlapping cDNA clones encoding a partial sequence of the cation-independent 215-kDa mannose 6-phosphate receptor have been identified by screening a fetal calf liver cDNA library with oligonucleotide probes. RNA hybridization analysis showed that the length of the mRNA is ~ 9.5 kilobases. Sequence analysis demonstrated that the clones consist of 4647 contiguous nucleotides and contain an open reading frame coding for a polypeptide of 1461 amino acids, which we estimate represents > 75% of the primary structure of the receptor. The deduced amino acid sequence indicates that the receptor has a carboxyl-terminal cytoplasmic domain of 163 amino acids that is rich in acidic residues, a 23-amino acid transmembrane segment, and an extracellular domain containing at least eight homologous repeats of ~ 145 amino acids. One of the repeats contains an additional 43-residue segment that is similar to the type II repeat of fibronectin. Each repeat contains a highly conserved 13-amino acid unit bordered by cysteine residues that may be functionally important.

AB - Four overlapping cDNA clones encoding a partial sequence of the cation-independent 215-kDa mannose 6-phosphate receptor have been identified by screening a fetal calf liver cDNA library with oligonucleotide probes. RNA hybridization analysis showed that the length of the mRNA is ~ 9.5 kilobases. Sequence analysis demonstrated that the clones consist of 4647 contiguous nucleotides and contain an open reading frame coding for a polypeptide of 1461 amino acids, which we estimate represents > 75% of the primary structure of the receptor. The deduced amino acid sequence indicates that the receptor has a carboxyl-terminal cytoplasmic domain of 163 amino acids that is rich in acidic residues, a 23-amino acid transmembrane segment, and an extracellular domain containing at least eight homologous repeats of ~ 145 amino acids. One of the repeats contains an additional 43-residue segment that is similar to the type II repeat of fibronectin. Each repeat contains a highly conserved 13-amino acid unit bordered by cysteine residues that may be functionally important.

UR - http://www.scopus.com/inward/record.url?scp=0023192368&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023192368&partnerID=8YFLogxK

U2 - 10.1073/pnas.84.8.2233

DO - 10.1073/pnas.84.8.2233

M3 - Article

VL - 84

SP - 2233

EP - 2237

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 8

ER -