Cloning of the Escherichia coli release factor 2 gene

C. T. Caskey, Wayne Forrester, W. Tate, C. D. Ward

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The protein release factor 2 (RF2) participates in Escherichia coli polypeptide chain termination with codon specificity (UAA or UGA). A colicin E1 recombinant identified in the Carbon and Clarke E. coli bank contains the protein release factor 2 gene. A 1.7-kilobase E. coli fragment has been subcloned into the plasmid pUC9 vector. Bacterial cells, containing the plasmid recombinant, produce elevated levels of protein release factor 2 as detected by an immune precipitation assay and in vitro measurement of UGA-directed peptide chain termination and [3H]UGA codon recognition.

Original languageEnglish (US)
Pages (from-to)365-368
Number of pages4
JournalJournal of Bacteriology
Volume158
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

Organism Cloning
Terminator Codon
Escherichia coli
Plasmids
Genes
Colicins
Peptides
Proteins
Immunoprecipitation
Carbon
In Vitro Techniques

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Caskey, C. T., Forrester, W., Tate, W., & Ward, C. D. (1984). Cloning of the Escherichia coli release factor 2 gene. Journal of Bacteriology, 158(1), 365-368.

Cloning of the Escherichia coli release factor 2 gene. / Caskey, C. T.; Forrester, Wayne; Tate, W.; Ward, C. D.

In: Journal of Bacteriology, Vol. 158, No. 1, 1984, p. 365-368.

Research output: Contribution to journalArticle

Caskey, CT, Forrester, W, Tate, W & Ward, CD 1984, 'Cloning of the Escherichia coli release factor 2 gene', Journal of Bacteriology, vol. 158, no. 1, pp. 365-368.
Caskey, C. T. ; Forrester, Wayne ; Tate, W. ; Ward, C. D. / Cloning of the Escherichia coli release factor 2 gene. In: Journal of Bacteriology. 1984 ; Vol. 158, No. 1. pp. 365-368.
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