Clonogenic endothelial progenitor cells are sensitive to oxidative stress

David Ingram, Theresa R. Krier, Laura E. Mead, Colleen McGuire, Daniel N. Prater, Janak Bhavsar, M. Reza Saadatzadeh, Khadijeh Bijangi-Vishehsaraei, Fang Li, Mervin Yoder, Laura Haneline

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

Endothelial progenitor cells (EPCs) circulate in the peripheral blood and reside in blood vessel walls. A hierarchy of EPCs exists where progenitors can be discriminated based on their clonogenic potential. EPCs are exposed to oxidative stress during vascular injury as residents of blood vessel walls or as circulating cells homing to sites of neovascularization. Given the links between oxidative injury, endothelial cell dysfunction, and vascular disease, we tested whether EPCs were sensitive to oxidative stress using newly developed clonogenic assays. Strikingly, in contrast to previous reports, we demonstrate that the most proliferative EPCs (high proliferative potential-endothelial colony-forming cells and low proliferative potential-endothelial colony-forming cells) had decreased clonogenic capacity after oxidant treatment. In addition, EPCs exhibited increased apoptosis and diminished tube-forming ability in vitro and in vivo in response to oxidative stress, which was directly linked to activation of a redox-dependent stress-induced kinase pathway. Thus, this study provides novel insights into the effect of oxidative stress on EPCs. Furthermore, this report outlines a framework for understanding how oxidative injury leads to vascular disease and potentially limits the efficacy of transplantation of EPCs into ischemic tissues enriched for reactive oxygen species and oxidized metabolites.

Original languageEnglish
Pages (from-to)297-304
Number of pages8
JournalStem Cells
Volume25
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Oxidative Stress
Vascular Diseases
Blood Vessels
Endothelial Progenitor Cells
Vascular System Injuries
Wounds and Injuries
Oxidants
Oxidation-Reduction
Reactive Oxygen Species
Phosphotransferases
Endothelial Cells
Transplantation
Apoptosis

Keywords

  • Endothelial cells
  • Mitogen-activated protein kinase kinase kinase 5
  • Oxidative stress
  • Stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Clonogenic endothelial progenitor cells are sensitive to oxidative stress. / Ingram, David; Krier, Theresa R.; Mead, Laura E.; McGuire, Colleen; Prater, Daniel N.; Bhavsar, Janak; Saadatzadeh, M. Reza; Bijangi-Vishehsaraei, Khadijeh; Li, Fang; Yoder, Mervin; Haneline, Laura.

In: Stem Cells, Vol. 25, No. 2, 02.2007, p. 297-304.

Research output: Contribution to journalArticle

Ingram, D, Krier, TR, Mead, LE, McGuire, C, Prater, DN, Bhavsar, J, Saadatzadeh, MR, Bijangi-Vishehsaraei, K, Li, F, Yoder, M & Haneline, L 2007, 'Clonogenic endothelial progenitor cells are sensitive to oxidative stress', Stem Cells, vol. 25, no. 2, pp. 297-304. https://doi.org/10.1634/stemcells.2006-0340
Ingram, David ; Krier, Theresa R. ; Mead, Laura E. ; McGuire, Colleen ; Prater, Daniel N. ; Bhavsar, Janak ; Saadatzadeh, M. Reza ; Bijangi-Vishehsaraei, Khadijeh ; Li, Fang ; Yoder, Mervin ; Haneline, Laura. / Clonogenic endothelial progenitor cells are sensitive to oxidative stress. In: Stem Cells. 2007 ; Vol. 25, No. 2. pp. 297-304.
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