Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy

Marilyn S. Bartlett, Michael P. Goheen, Chao-Hung Lee, Margaret M. Shaw, Michelle M. Durkin, James W. Smith

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Studies of the association of rat-origin Pneumocystis carinii with culture cells were performed both to learn more about the role of cells in P. carinii culture and to evaluate additional cell lines in an effort to improve culture methods. Proliferation of trophozoites of P. carinii from rat lung in cultures with six lung cell lines was demonstrated by light microscopic evaluations of both Giemsa-stained and immune-specific-stained culture samples. Scanning electron microscopy and transmission electron microscopy were used to study the organism's interaction with culture cells and demonstrated a close association of P. carinii with cells in cell lines that supported growth. Proliferation with the MVILU line was suboptimal and there was less organism interaction with these cells than with other cell lines that allowed proliferation. Two cell lines evaluated, Chinese Hamster ovary CHOKI and CHOLEKI, did not allow proliferation and had no association of P. carinii with cells. Scanning and transmission electron micrographs demonstrated the close association of organisms with rat fetal lung (RFL), human embryonic lung (HEL), human diploid lung (HFL), and feline embryonic lung (AKD) culture cells. It appears that the association of rat-origin P. carinii with cells is essential for parasite proliferation in short-term culture.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalParasitology Research
Volume80
Issue number3
DOIs
StatePublished - Mar 1994

Fingerprint

Pneumocystis carinii
light microscopy
electron microscopy
Electron Microscopy
cell culture
Cell Culture Techniques
lungs
Light
Lung
cell lines
rats
Cell Line
cells
organisms
Trophozoites
trophozoites
Felidae
Chinese hamsters
Cricetulus
Diploidy

ASJC Scopus subject areas

  • Parasitology

Cite this

Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy. / Bartlett, Marilyn S.; Goheen, Michael P.; Lee, Chao-Hung; Shaw, Margaret M.; Durkin, Michelle M.; Smith, James W.

In: Parasitology Research, Vol. 80, No. 3, 03.1994, p. 208-215.

Research output: Contribution to journalArticle

Bartlett, Marilyn S. ; Goheen, Michael P. ; Lee, Chao-Hung ; Shaw, Margaret M. ; Durkin, Michelle M. ; Smith, James W. / Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy. In: Parasitology Research. 1994 ; Vol. 80, No. 3. pp. 208-215.
@article{e22f7ebd3d2e43ee9df437550282317b,
title = "Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy",
abstract = "Studies of the association of rat-origin Pneumocystis carinii with culture cells were performed both to learn more about the role of cells in P. carinii culture and to evaluate additional cell lines in an effort to improve culture methods. Proliferation of trophozoites of P. carinii from rat lung in cultures with six lung cell lines was demonstrated by light microscopic evaluations of both Giemsa-stained and immune-specific-stained culture samples. Scanning electron microscopy and transmission electron microscopy were used to study the organism's interaction with culture cells and demonstrated a close association of P. carinii with cells in cell lines that supported growth. Proliferation with the MVILU line was suboptimal and there was less organism interaction with these cells than with other cell lines that allowed proliferation. Two cell lines evaluated, Chinese Hamster ovary CHOKI and CHOLEKI, did not allow proliferation and had no association of P. carinii with cells. Scanning and transmission electron micrographs demonstrated the close association of organisms with rat fetal lung (RFL), human embryonic lung (HEL), human diploid lung (HFL), and feline embryonic lung (AKD) culture cells. It appears that the association of rat-origin P. carinii with cells is essential for parasite proliferation in short-term culture.",
author = "Bartlett, {Marilyn S.} and Goheen, {Michael P.} and Chao-Hung Lee and Shaw, {Margaret M.} and Durkin, {Michelle M.} and Smith, {James W.}",
year = "1994",
month = "3",
doi = "10.1007/BF00932676",
language = "English",
volume = "80",
pages = "208--215",
journal = "Zeitschrift fur Parasitenkunde",
issn = "0932-0113",
publisher = "Springer Verlag",
number = "3",

}

TY - JOUR

T1 - Close association of Pneumocystis carinii from infected rat lung with culture cells as shown by light and electron microscopy

AU - Bartlett, Marilyn S.

AU - Goheen, Michael P.

AU - Lee, Chao-Hung

AU - Shaw, Margaret M.

AU - Durkin, Michelle M.

AU - Smith, James W.

PY - 1994/3

Y1 - 1994/3

N2 - Studies of the association of rat-origin Pneumocystis carinii with culture cells were performed both to learn more about the role of cells in P. carinii culture and to evaluate additional cell lines in an effort to improve culture methods. Proliferation of trophozoites of P. carinii from rat lung in cultures with six lung cell lines was demonstrated by light microscopic evaluations of both Giemsa-stained and immune-specific-stained culture samples. Scanning electron microscopy and transmission electron microscopy were used to study the organism's interaction with culture cells and demonstrated a close association of P. carinii with cells in cell lines that supported growth. Proliferation with the MVILU line was suboptimal and there was less organism interaction with these cells than with other cell lines that allowed proliferation. Two cell lines evaluated, Chinese Hamster ovary CHOKI and CHOLEKI, did not allow proliferation and had no association of P. carinii with cells. Scanning and transmission electron micrographs demonstrated the close association of organisms with rat fetal lung (RFL), human embryonic lung (HEL), human diploid lung (HFL), and feline embryonic lung (AKD) culture cells. It appears that the association of rat-origin P. carinii with cells is essential for parasite proliferation in short-term culture.

AB - Studies of the association of rat-origin Pneumocystis carinii with culture cells were performed both to learn more about the role of cells in P. carinii culture and to evaluate additional cell lines in an effort to improve culture methods. Proliferation of trophozoites of P. carinii from rat lung in cultures with six lung cell lines was demonstrated by light microscopic evaluations of both Giemsa-stained and immune-specific-stained culture samples. Scanning electron microscopy and transmission electron microscopy were used to study the organism's interaction with culture cells and demonstrated a close association of P. carinii with cells in cell lines that supported growth. Proliferation with the MVILU line was suboptimal and there was less organism interaction with these cells than with other cell lines that allowed proliferation. Two cell lines evaluated, Chinese Hamster ovary CHOKI and CHOLEKI, did not allow proliferation and had no association of P. carinii with cells. Scanning and transmission electron micrographs demonstrated the close association of organisms with rat fetal lung (RFL), human embryonic lung (HEL), human diploid lung (HFL), and feline embryonic lung (AKD) culture cells. It appears that the association of rat-origin P. carinii with cells is essential for parasite proliferation in short-term culture.

UR - http://www.scopus.com/inward/record.url?scp=0028296303&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028296303&partnerID=8YFLogxK

U2 - 10.1007/BF00932676

DO - 10.1007/BF00932676

M3 - Article

VL - 80

SP - 208

EP - 215

JO - Zeitschrift fur Parasitenkunde

JF - Zeitschrift fur Parasitenkunde

SN - 0932-0113

IS - 3

ER -