Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis

Joseph A. Karam, Rasesh Y. Parikh, Dhananjaya Nayak, David Rosenkranz, Vamsi K. Gangaraju, Ronald Wek

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Piwi-interacting RNAs (piRNAs) are 26-30-nucleotide germ line-specific small non-coding RNAs that have evolutionarily conserved function in mobile genetic element (transposons) silencing and maintenance of genome integrity. Drosophila Hsp70/90-organizing protein homolog (Hop), a co-chaperone, interacts with piRNA-binding protein Piwi and mediates silencing of phenotypic variations. However, it is not known whether Hop has a direct role in piRNA biogenesis and transposon silencing. Here, we show that knockdown of Hop in the germ line nurse cells (GLKD) of Drosophila ovaries leads to activation of transposons. Hop GLKD females can lay eggs at the same rate as wild-type counterparts, but the eggs do not hatch into larvae. Hop GLKD leads to the accumulation of γ-H2Av foci in the germ line, indicating increased DNA damage in the ovary. We also show that Hop GLKD-induced transposon up-regulation is due to inefficient piRNA biogenesis. Based on these results, we conclude that Hop is a critical component of the piRNA pathway and that it maintains genome integrity by silencing transposons.

Original languageEnglish (US)
Pages (from-to)6039-6046
Number of pages8
JournalJournal of Biological Chemistry
Volume292
Issue number15
DOIs
StatePublished - Apr 14 2017

Fingerprint

Small Interfering RNA
Germ Cells
Proteins
Eggs
Drosophila
Ovary
Genes
Genome
Interspersed Repetitive Sequences
Hatches
Small Untranslated RNA
RNA-Binding Proteins
DNA Damage
Larva
Up-Regulation
Nucleotides
Chemical activation
Nurses
Maintenance
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis. / Karam, Joseph A.; Parikh, Rasesh Y.; Nayak, Dhananjaya; Rosenkranz, David; Gangaraju, Vamsi K.; Wek, Ronald.

In: Journal of Biological Chemistry, Vol. 292, No. 15, 14.04.2017, p. 6039-6046.

Research output: Contribution to journalArticle

Karam, Joseph A. ; Parikh, Rasesh Y. ; Nayak, Dhananjaya ; Rosenkranz, David ; Gangaraju, Vamsi K. ; Wek, Ronald. / Co-chaperone Hsp70/Hsp90-organizing protein (Hop) is required for transposon silencing and Piwi-interacting RNA (piRNA) biogenesis. In: Journal of Biological Chemistry. 2017 ; Vol. 292, No. 15. pp. 6039-6046.
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