Co-existence of necrosis and apoptosis in rat hippocampus following transient forebrain ischemia

Yuan Shan Zeng, Zao C. Xu

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Morphological changes of CA1 and CA3 pyramidal neurons in rat hippocampus at different intervals following transient forebrain ischemia were examined to determine the nature of post-ischemic cell death in these regions. In the CA1 region, swelling of small dendrites occurred at ~24 h reperfusion. At ~48 h reperfusion, swelling was found in large dendrites of many CA1 neurons and the mitochondria and endoplasmic reticulum (ER) were dilated. A small portion of neurons showed chromatin aggregation and nuclear indentation without swelling signs. At ~60 h reperfusion, swelling of somata was evident in many neurons. Large dense chromatin clumps with round or ovoid contour were found in other neurons. At 72 and 96 h after ischemia, many large vacuoles and glias with active phagocytosis were observed. At 7 days after ischemia, the tissue was compact and many glias were found in the region. Most of the CA3 neurons had normal appearance after ischemia. A total of 5-10% CA3 neurons exhibited shrinking nuclei and chromatin aggregation at ~24 h reperfusion. The number of these neurons decreased overtime and disappeared at 72 h after ischemia. These results demonstrate the co-existence of necrosis and apoptosis in the CA1 region after transient forebrain ischemia. Most CA3 neurons remained intact after ischemia while a small portion of them showed apoptotic cell death. Copyright (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society.

Original languageEnglish (US)
Pages (from-to)113-125
Number of pages13
JournalNeuroscience Research
Volume37
Issue number2
DOIs
StatePublished - Jun 1 2000

Fingerprint

Prosencephalon
Hippocampus
Necrosis
Ischemia
Apoptosis
Neurons
Reperfusion
Chromatin
Dendrites
Cell Death
Pyramidal Cells
Carisoprodol
Neurosciences
Vacuoles
Phagocytosis
Endoplasmic Reticulum
Japan
Mitochondria

Keywords

  • Apoptosis
  • Cerebral ischemia
  • Electron microscopy
  • Hippocampus
  • Necrosis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Co-existence of necrosis and apoptosis in rat hippocampus following transient forebrain ischemia. / Zeng, Yuan Shan; Xu, Zao C.

In: Neuroscience Research, Vol. 37, No. 2, 01.06.2000, p. 113-125.

Research output: Contribution to journalArticle

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AB - Morphological changes of CA1 and CA3 pyramidal neurons in rat hippocampus at different intervals following transient forebrain ischemia were examined to determine the nature of post-ischemic cell death in these regions. In the CA1 region, swelling of small dendrites occurred at ~24 h reperfusion. At ~48 h reperfusion, swelling was found in large dendrites of many CA1 neurons and the mitochondria and endoplasmic reticulum (ER) were dilated. A small portion of neurons showed chromatin aggregation and nuclear indentation without swelling signs. At ~60 h reperfusion, swelling of somata was evident in many neurons. Large dense chromatin clumps with round or ovoid contour were found in other neurons. At 72 and 96 h after ischemia, many large vacuoles and glias with active phagocytosis were observed. At 7 days after ischemia, the tissue was compact and many glias were found in the region. Most of the CA3 neurons had normal appearance after ischemia. A total of 5-10% CA3 neurons exhibited shrinking nuclei and chromatin aggregation at ~24 h reperfusion. The number of these neurons decreased overtime and disappeared at 72 h after ischemia. These results demonstrate the co-existence of necrosis and apoptosis in the CA1 region after transient forebrain ischemia. Most CA3 neurons remained intact after ischemia while a small portion of them showed apoptotic cell death. Copyright (C) 2000 Elsevier Science Ireland Ltd and the Japan Neuroscience Society.

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