While human malignant mesothelioma is extremely resistant to chemotherapy, its intrinsic resistance mechanisms remain largely unknown. In this study, we used normal human mesothelial cells and 5 human mesothelioma cell lines not previously exposed to chemotherapeutic agents to demonstrate that the mRNA for the multidrug resistance associated protein (MRP) and γ- glutamylcysteine synthetase (γ-GCS(h)) heavy subunit genes, but not the P- glycoprotein (MDR1) gene, are co-ordinately over-expressed in mesothelioma cell lines. Expression of MRP as detected with an anti-MRP antibody correlated with decreased doxorubicin accumulation and resistance of mesothelioma cells to this drug. Our results strongly suggest roles for MRP and γ-GCS(h) in chemoresistance in mesotheliomas.
|Original language||English (US)|
|Number of pages||5|
|Journal||International Journal of Cancer|
|State||Published - Mar 2 1998|
ASJC Scopus subject areas
- Cancer Research