CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model

C. Steiner-Oliveira, L. K A Rodrigues, T. M. Parisotto, C. M. Sousa E Silva, Anderson Hara, M. Nobre-Dos-Santos

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

An increase in the dental caries prevalence on root surfaces has been observed mainly in elderly. This research assessed, in vitro, the effectiveness of a pulsed CO2 (λ = 10.6 μm) laser associated or not with fluoride, in reducing human root dentine demineralization in conditions that mimic an oral high cariogenic challenge. After sterilization, root dentine specimens were randomly assigned into 6 groups (n = 30), in triplicate. The groups were Control (C), Streptococcus mutans (SM), Fluoride (F), Laser (L), Fluoride + laser (FL), and Laser + fluoride (LF). Except for the control group, all the specimens were inoculated with SM and immersed 3 times a day in a 40% sucrose bath. After a 7-day cariogenic challenge, the mineral loss and lesion depth were evaluated by transverse microradiography and fluoride in the biofilm was determined using an ion-selective electrode. Results were statistically analyzed by analysis of variance, at 5% of significance level. For groups C, SM, F, L, FL and LF, the means (standard-deviation) of mineral loss were 816.3 (552.5)a, 3291.5 (1476.2)c, 2508.5 (1240.5)bc, 2916.2 (1323.7)c, 1839.7 (815.2)b and 1955.0 (1001.4) b, respectively; while lesion depths were 39.6 (22.8)a, 103.1 (38.9)c, 90.3 (44.6)bc, 91.7 (27.0)bc, 73.3 (26.6)b, 75.1 (35.2)b, respectively (different superscript letters indicate significant differences among groups). In conclusion, irradiation of root dentine with a pulsed CO2 laser at fluency of 12.0 J/cm2 was able to inhibit root surface demineralization only when associated with fluoride. No synergy effect on the inhibition of root dentine mineral loss was provided by the combination of fluoride application and laser irradiation.

Original languageEnglish (US)
Pages (from-to)1838-1843
Number of pages6
JournalLaser Physics
Volume20
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

fluorides
Lasers
streptococcus
lasers
Minerals
minerals
lesions
Biofilms
Sugar (sucrose)
Laser beam effects
Analysis of variance (ANOVA)
Pulsed lasers
ion selective electrodes
analysis of variance
biofilms
irradiation
sucrose
Irradiation
baths
standard deviation

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Industrial and Manufacturing Engineering

Cite this

Steiner-Oliveira, C., Rodrigues, L. K. A., Parisotto, T. M., Sousa E Silva, C. M., Hara, A., & Nobre-Dos-Santos, M. (2010). CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model. Laser Physics, 20(9), 1838-1843. https://doi.org/10.1134/S1054660X10170147

CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model. / Steiner-Oliveira, C.; Rodrigues, L. K A; Parisotto, T. M.; Sousa E Silva, C. M.; Hara, Anderson; Nobre-Dos-Santos, M.

In: Laser Physics, Vol. 20, No. 9, 09.2010, p. 1838-1843.

Research output: Contribution to journalArticle

Steiner-Oliveira, C, Rodrigues, LKA, Parisotto, TM, Sousa E Silva, CM, Hara, A & Nobre-Dos-Santos, M 2010, 'CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model', Laser Physics, vol. 20, no. 9, pp. 1838-1843. https://doi.org/10.1134/S1054660X10170147
Steiner-Oliveira C, Rodrigues LKA, Parisotto TM, Sousa E Silva CM, Hara A, Nobre-Dos-Santos M. CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model. Laser Physics. 2010 Sep;20(9):1838-1843. https://doi.org/10.1134/S1054660X10170147
Steiner-Oliveira, C. ; Rodrigues, L. K A ; Parisotto, T. M. ; Sousa E Silva, C. M. ; Hara, Anderson ; Nobre-Dos-Santos, M. / CO2 laser and fluoride on the inhibition of root caries-an in vitro microbial model. In: Laser Physics. 2010 ; Vol. 20, No. 9. pp. 1838-1843.
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abstract = "An increase in the dental caries prevalence on root surfaces has been observed mainly in elderly. This research assessed, in vitro, the effectiveness of a pulsed CO2 (λ = 10.6 μm) laser associated or not with fluoride, in reducing human root dentine demineralization in conditions that mimic an oral high cariogenic challenge. After sterilization, root dentine specimens were randomly assigned into 6 groups (n = 30), in triplicate. The groups were Control (C), Streptococcus mutans (SM), Fluoride (F), Laser (L), Fluoride + laser (FL), and Laser + fluoride (LF). Except for the control group, all the specimens were inoculated with SM and immersed 3 times a day in a 40{\%} sucrose bath. After a 7-day cariogenic challenge, the mineral loss and lesion depth were evaluated by transverse microradiography and fluoride in the biofilm was determined using an ion-selective electrode. Results were statistically analyzed by analysis of variance, at 5{\%} of significance level. For groups C, SM, F, L, FL and LF, the means (standard-deviation) of mineral loss were 816.3 (552.5)a, 3291.5 (1476.2)c, 2508.5 (1240.5)bc, 2916.2 (1323.7)c, 1839.7 (815.2)b and 1955.0 (1001.4) b, respectively; while lesion depths were 39.6 (22.8)a, 103.1 (38.9)c, 90.3 (44.6)bc, 91.7 (27.0)bc, 73.3 (26.6)b, 75.1 (35.2)b, respectively (different superscript letters indicate significant differences among groups). In conclusion, irradiation of root dentine with a pulsed CO2 laser at fluency of 12.0 J/cm2 was able to inhibit root surface demineralization only when associated with fluoride. No synergy effect on the inhibition of root dentine mineral loss was provided by the combination of fluoride application and laser irradiation.",
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