Coexistence of two types of ventricular fibrillation during acute regional ischemia in rabbit ventricle

Yen Bin Liu, Hui Nam Pak, Scott T. Lamp, Yuji Okuyama, Hideki Hayashi, Tsu Juey Wu, James N. Weiss, Peng-Sheng Chen, Shien-Fong Lin

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Two Types of VF in Regional Ischemia. Introduction: We previously reported that a normal ventricle can demonstrate two types of ventricular fibrillation (VF), depending on the underlying electrophysiologic characteristics at the time of VF induction. We hypothesize that the two types of VF can coexist in acutely ischemic ventricles. Methods and Results: Optical mapping studies were performed with di-4ANEPPS in 15 Langendorff-perfused rabbit hearts. Coronary artery branches were ligated to create regional ischemia in 10 hearts. Action potential duration measured to 50% repolarization (APD50) during ischemia showed an area with uniformly shortened APD50 (zone 1), an area with normal or lengthened APD50 (Zone 3), and an area in between with an APD50 gradient (zone 2). Ischemia flattened APD restitution (APDR) slope and reduced conduction velocity in zone 1, creating a condition for type II VF. APDR steepened and the conduction velocity changed little in the nonischemic zone (zone 3), creating a condition for type I VF. During induced VF, the dominant frequency in zones 2 and 3 progressively increased after ischemia onset. The dominant frequency in zone 1 (ischemic zone) first decreased and then slightly increased but typically remained less than the dominant frequency in zone 3. The number of wavebreaks increased with time in all three zones (baseline: 4.3 ± 1.5; 30 min: 11.7 ± 5.6; 60 min: 15.6 ± 11 per frame; P <0.01). Conclusion: Two types of VF can coexist during acute regional ischemia. Both ischemic and nonischemic regions develop proarrhythmic changes during regional ischemia, thus contributing to increased ventricular vulnerability to VF and sudden death during acute coronary occlusion.

Original languageEnglish (US)
Pages (from-to)1433-1440
Number of pages8
JournalJournal of Cardiovascular Electrophysiology
Volume15
Issue number12
DOIs
StatePublished - Dec 2004
Externally publishedYes

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Ventricular Fibrillation
Ischemia
Rabbits
pamidronate
Coronary Occlusion
Sudden Death
Action Potentials
Coronary Vessels

Keywords

  • Acute myocardial infarction
  • Arrhythmia
  • Ischemia
  • Optical mapping
  • Ventricular fibrillation

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology

Cite this

Coexistence of two types of ventricular fibrillation during acute regional ischemia in rabbit ventricle. / Liu, Yen Bin; Pak, Hui Nam; Lamp, Scott T.; Okuyama, Yuji; Hayashi, Hideki; Wu, Tsu Juey; Weiss, James N.; Chen, Peng-Sheng; Lin, Shien-Fong.

In: Journal of Cardiovascular Electrophysiology, Vol. 15, No. 12, 12.2004, p. 1433-1440.

Research output: Contribution to journalArticle

Liu, Yen Bin ; Pak, Hui Nam ; Lamp, Scott T. ; Okuyama, Yuji ; Hayashi, Hideki ; Wu, Tsu Juey ; Weiss, James N. ; Chen, Peng-Sheng ; Lin, Shien-Fong. / Coexistence of two types of ventricular fibrillation during acute regional ischemia in rabbit ventricle. In: Journal of Cardiovascular Electrophysiology. 2004 ; Vol. 15, No. 12. pp. 1433-1440.
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AU - Liu, Yen Bin

AU - Pak, Hui Nam

AU - Lamp, Scott T.

AU - Okuyama, Yuji

AU - Hayashi, Hideki

AU - Wu, Tsu Juey

AU - Weiss, James N.

AU - Chen, Peng-Sheng

AU - Lin, Shien-Fong

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AB - Two Types of VF in Regional Ischemia. Introduction: We previously reported that a normal ventricle can demonstrate two types of ventricular fibrillation (VF), depending on the underlying electrophysiologic characteristics at the time of VF induction. We hypothesize that the two types of VF can coexist in acutely ischemic ventricles. Methods and Results: Optical mapping studies were performed with di-4ANEPPS in 15 Langendorff-perfused rabbit hearts. Coronary artery branches were ligated to create regional ischemia in 10 hearts. Action potential duration measured to 50% repolarization (APD50) during ischemia showed an area with uniformly shortened APD50 (zone 1), an area with normal or lengthened APD50 (Zone 3), and an area in between with an APD50 gradient (zone 2). Ischemia flattened APD restitution (APDR) slope and reduced conduction velocity in zone 1, creating a condition for type II VF. APDR steepened and the conduction velocity changed little in the nonischemic zone (zone 3), creating a condition for type I VF. During induced VF, the dominant frequency in zones 2 and 3 progressively increased after ischemia onset. The dominant frequency in zone 1 (ischemic zone) first decreased and then slightly increased but typically remained less than the dominant frequency in zone 3. The number of wavebreaks increased with time in all three zones (baseline: 4.3 ± 1.5; 30 min: 11.7 ± 5.6; 60 min: 15.6 ± 11 per frame; P <0.01). Conclusion: Two types of VF can coexist during acute regional ischemia. Both ischemic and nonischemic regions develop proarrhythmic changes during regional ischemia, thus contributing to increased ventricular vulnerability to VF and sudden death during acute coronary occlusion.

KW - Acute myocardial infarction

KW - Arrhythmia

KW - Ischemia

KW - Optical mapping

KW - Ventricular fibrillation

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