Cold ischaemic preservation of human uterine tissue

Krishen Sieunarine, Ian Lindsay, Laszlo Ungar, Giuseppe Del Priore, J. Richard Smith

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Uterine transplantation may be a possible treatment option in the future for absolute uterine infertility. The tolerance of human uterine tissue to cold ischemic preservation is one of the issues that need to be resolved. The objective of this study was to assess the morphological changes in human uterine tissue after cold ischemic preservation in a transplant solution. Small tissue samples of human uteri were subjected to cold ischemia (2-8°C for up to 48 hours) in Celsior transplant solution. Histological analysis by light and electron microscopy was used to assess evidence of cold ischemic injury. Histological examination did not show any major changes of the uterine tissue after 48-hour cold preservation; whereas, electron microscopy after 24 hours confirmed unchanged structural integrity of the uterine myoendometrium. The human uterus is morphologically resistant toward cold ischemia in Celsior preservation solution for up to 24 hours and may be suitable for transplantation purposes.

Original languageEnglish (US)
Pages (from-to)366-372
Number of pages7
JournalInternational Surgery
Volume93
Issue number6
StatePublished - 2009
Externally publishedYes

Fingerprint

Cold Ischemia
Uterus
Electron Microscopy
Transplantation
Transplants
Infertility
Light
Celsior
Therapeutics
Cold Injury

Keywords

  • Cold ischemia
  • Human uterine transplantation
  • Uterine tissue preservation

ASJC Scopus subject areas

  • Surgery

Cite this

Sieunarine, K., Lindsay, I., Ungar, L., Del Priore, G., & Smith, J. R. (2009). Cold ischaemic preservation of human uterine tissue. International Surgery, 93(6), 366-372.

Cold ischaemic preservation of human uterine tissue. / Sieunarine, Krishen; Lindsay, Ian; Ungar, Laszlo; Del Priore, Giuseppe; Smith, J. Richard.

In: International Surgery, Vol. 93, No. 6, 2009, p. 366-372.

Research output: Contribution to journalArticle

Sieunarine, K, Lindsay, I, Ungar, L, Del Priore, G & Smith, JR 2009, 'Cold ischaemic preservation of human uterine tissue', International Surgery, vol. 93, no. 6, pp. 366-372.
Sieunarine K, Lindsay I, Ungar L, Del Priore G, Smith JR. Cold ischaemic preservation of human uterine tissue. International Surgery. 2009;93(6):366-372.
Sieunarine, Krishen ; Lindsay, Ian ; Ungar, Laszlo ; Del Priore, Giuseppe ; Smith, J. Richard. / Cold ischaemic preservation of human uterine tissue. In: International Surgery. 2009 ; Vol. 93, No. 6. pp. 366-372.
@article{0d0d3991eb694063a8e7ba950b484ebf,
title = "Cold ischaemic preservation of human uterine tissue",
abstract = "Uterine transplantation may be a possible treatment option in the future for absolute uterine infertility. The tolerance of human uterine tissue to cold ischemic preservation is one of the issues that need to be resolved. The objective of this study was to assess the morphological changes in human uterine tissue after cold ischemic preservation in a transplant solution. Small tissue samples of human uteri were subjected to cold ischemia (2-8°C for up to 48 hours) in Celsior transplant solution. Histological analysis by light and electron microscopy was used to assess evidence of cold ischemic injury. Histological examination did not show any major changes of the uterine tissue after 48-hour cold preservation; whereas, electron microscopy after 24 hours confirmed unchanged structural integrity of the uterine myoendometrium. The human uterus is morphologically resistant toward cold ischemia in Celsior preservation solution for up to 24 hours and may be suitable for transplantation purposes.",
keywords = "Cold ischemia, Human uterine transplantation, Uterine tissue preservation",
author = "Krishen Sieunarine and Ian Lindsay and Laszlo Ungar and {Del Priore}, Giuseppe and Smith, {J. Richard}",
year = "2009",
language = "English (US)",
volume = "93",
pages = "366--372",
journal = "International Surgery",
issn = "0020-8868",
publisher = "International College of Surgeons",
number = "6",

}

TY - JOUR

T1 - Cold ischaemic preservation of human uterine tissue

AU - Sieunarine, Krishen

AU - Lindsay, Ian

AU - Ungar, Laszlo

AU - Del Priore, Giuseppe

AU - Smith, J. Richard

PY - 2009

Y1 - 2009

N2 - Uterine transplantation may be a possible treatment option in the future for absolute uterine infertility. The tolerance of human uterine tissue to cold ischemic preservation is one of the issues that need to be resolved. The objective of this study was to assess the morphological changes in human uterine tissue after cold ischemic preservation in a transplant solution. Small tissue samples of human uteri were subjected to cold ischemia (2-8°C for up to 48 hours) in Celsior transplant solution. Histological analysis by light and electron microscopy was used to assess evidence of cold ischemic injury. Histological examination did not show any major changes of the uterine tissue after 48-hour cold preservation; whereas, electron microscopy after 24 hours confirmed unchanged structural integrity of the uterine myoendometrium. The human uterus is morphologically resistant toward cold ischemia in Celsior preservation solution for up to 24 hours and may be suitable for transplantation purposes.

AB - Uterine transplantation may be a possible treatment option in the future for absolute uterine infertility. The tolerance of human uterine tissue to cold ischemic preservation is one of the issues that need to be resolved. The objective of this study was to assess the morphological changes in human uterine tissue after cold ischemic preservation in a transplant solution. Small tissue samples of human uteri were subjected to cold ischemia (2-8°C for up to 48 hours) in Celsior transplant solution. Histological analysis by light and electron microscopy was used to assess evidence of cold ischemic injury. Histological examination did not show any major changes of the uterine tissue after 48-hour cold preservation; whereas, electron microscopy after 24 hours confirmed unchanged structural integrity of the uterine myoendometrium. The human uterus is morphologically resistant toward cold ischemia in Celsior preservation solution for up to 24 hours and may be suitable for transplantation purposes.

KW - Cold ischemia

KW - Human uterine transplantation

KW - Uterine tissue preservation

UR - http://www.scopus.com/inward/record.url?scp=77449149782&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77449149782&partnerID=8YFLogxK

M3 - Article

C2 - 20085047

AN - SCOPUS:77449149782

VL - 93

SP - 366

EP - 372

JO - International Surgery

JF - International Surgery

SN - 0020-8868

IS - 6

ER -