Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo

P. J. Critser, S. T. Kreger, S. L. Voytik-Harbin, Mervin Yoder

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Developing tissue engineering approaches to generate functional vascular networks is important for improving treatments of peripheral and cardiovascular disease. Endothelial colony forming cells (ECFCs) are an endothelial progenitor cell (EPC) population defined by high proliferative potential and an ability to vascularize collagen-based matrices in vivo. Little is known regarding how physical properties of the local cell microenvironment guide vessel formation following EPC transplantation. In vitro evidence suggests that collagen matrix stiffness may modulate EPC vessel formation. The present study determined the ability of 3D collagen matrix physical properties, varied by changing collagen concentration, to influence ECFC vasculogenesis in vivo. Human umbilical cord blood ECFCs were cultured within matrices for 18. h in vitro and then fixed for in vitro analysis or implanted subcutaneously into the flank of immunodeficient mice for 14. days. We report that increasing collagen concentration significantly decreased ECFC derived vessels per area (density), but significantly increased vessel sizes (total cross sectional area). These results demonstrate that the physical properties of collagen matrices influence ECFC vasculogenesis in vivo and that by modulating these properties, one can guide vascularization.

Original languageEnglish
Pages (from-to)23-30
Number of pages8
JournalMicrovascular Research
Volume80
Issue number1
DOIs
StatePublished - Jul 2010

Fingerprint

Collagen
Physical properties
Endothelial cells
Cellular Microenvironment
Cell Transplantation
Stiffness matrix
Tissue Engineering
Fetal Blood
Tissue engineering
Blood Vessels
Cultured Cells
Blood
Cardiovascular Diseases
Cells
Population
Endothelial Progenitor Cells
In Vitro Techniques
Therapeutics

Keywords

  • Collagen
  • ECM (extracellular matrix)
  • Endothelial progenitor cell (EPC)
  • In vivo
  • Mechanical properties
  • Vascular graft

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology

Cite this

Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo. / Critser, P. J.; Kreger, S. T.; Voytik-Harbin, S. L.; Yoder, Mervin.

In: Microvascular Research, Vol. 80, No. 1, 07.2010, p. 23-30.

Research output: Contribution to journalArticle

Critser, P. J. ; Kreger, S. T. ; Voytik-Harbin, S. L. ; Yoder, Mervin. / Collagen matrix physical properties modulate endothelial colony forming cell-derived vessels in vivo. In: Microvascular Research. 2010 ; Vol. 80, No. 1. pp. 23-30.
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