Collagen-polymer guidance of vessel network formation and stabilization by endothelial colony forming cells in vitro

Catherine F. Whittington, Mervin Yoder, Sherry L. Voytik-Harbin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Vessel morphogenesis is vital to regenerative medicine strategies. Here, collagen polymers, specified by intermolecular cross-link composition, are used to independently vary microstructure (fibril density, interfibril branching) and physical properties (stiffness) to guide 3D vessel network formation by endothelial colony forming cells (ECFC) in vitro. Increasing stiffness, by modulation of fibril density or interfibril branching, increases vessel diameter, length and branching. Oligomer matrices also induce vessel stabilization via type IV collagen deposition. This work shows that ECFC vessel formation depends on the interplay of collagen microstructure and physical properties and names oligomers and intermolecular cross-links as key design parameters for vascular-inductive matrices.

Original languageEnglish
Pages (from-to)1135-1149
Number of pages15
JournalMacromolecular Bioscience
Volume13
Issue number9
DOIs
StatePublished - Sep 2013

Fingerprint

Oligomers
Collagen
Polymers
Stabilization
Physical properties
Stiffness
Microstructure
Regenerative Medicine
Collagen Type IV
Morphogenesis
Names
Blood Vessels
Modulation
Chemical analysis
In Vitro Techniques

Keywords

  • Endothelial colony forming cells
  • Microstructure
  • Oligomers
  • Type I collagen
  • Vasculogenesis

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Collagen-polymer guidance of vessel network formation and stabilization by endothelial colony forming cells in vitro. / Whittington, Catherine F.; Yoder, Mervin; Voytik-Harbin, Sherry L.

In: Macromolecular Bioscience, Vol. 13, No. 9, 09.2013, p. 1135-1149.

Research output: Contribution to journalArticle

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