Collagen prolyl 4-hydroxylase is up-regulated in an acute bladder outlet obstruction

Sang Don Lee, Cem Akbal, Rosalia Miseeri, Chaeyong Jung, Richard Rink, Martin Kaefer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objective: Compliance is primarily related to extracellular matrix deposition, and prolyl 4-hydroxylase (P4Hs) plays a critical role in the synthesis of the matrix. To study the alteration of P4Hs, under the influence of variable hydrostatic pressure, a novel pressure device was used to expose human bladder smooth muscle cells (HBSMC) and fibroblasts (HBF) to pressures in the physiologic range. We then studied acute obstructed porcine bladder tissues to see if changes can also be seen after in-vitro obstruction. Materials and methods: HBSMC and HBF were exposed to pressures at 0, 20 and 40 cmH2O for up to 72 h. In-vivo studies were carried out next, using six normal (control) and five obstructed porcine bladders. Pigs were exposed to a consistent hydrostatic pressure of ≤20 cm for 24 h after ligation of the urethra. We used 2-DE to compare protein profiling of HBSMC under normal and increased pressures. Other analyses were used to detect molecular alteration and altered expression of mRNA for P4Hs. Results: We identified 437 proteins from 476 spots (91.8%) obtained from HBSMC that were differentially expressed under normal and increased pressures. Under increased pressure, 48 unique proteins were significantly increased or decreased, and a prominent protein regulating extracellular matrix synthesis highly correlated with P4Hs. The exposure of both HBSMC and HBF to a sustained hydrostatic pressure resulted in the increased expression of P4Hs in a time- and pressure-dependent manner. In vivo, P4Hs expression was also significantly increased in the obstructed group. Conclusions: P4Hs is up-regulated, in the human bladder, time and pressure dependently. The alteration of P4Hs over a short period may significantly influence the synthesis of extracellular matrix in vivo and lead to decreased compliance. Our results also support the concept that bladder outlet obstruction, with resultant pressures of 40 cmH2O or less, results in molecular changes consistent with decreased compliance.

Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalJournal of Pediatric Urology
Volume2
Issue number4
DOIs
StatePublished - Aug 2006

Fingerprint

Prolyl Hydroxylases
Urinary Bladder Neck Obstruction
Collagen
Urinary Bladder
Pressure
Smooth Muscle Myocytes
Hydrostatic Pressure
Compliance
Swine
Fibroblasts
Extracellular Matrix
Proteins
Extracellular Matrix Proteins
Urethra
Ligation
Equipment and Supplies
Messenger RNA

Keywords

  • Bladder outlet obstruction
  • Bladder smooth muscle
  • Prolyl 4-hydroxylase

ASJC Scopus subject areas

  • Urology
  • Pediatrics, Perinatology, and Child Health

Cite this

Collagen prolyl 4-hydroxylase is up-regulated in an acute bladder outlet obstruction. / Lee, Sang Don; Akbal, Cem; Miseeri, Rosalia; Jung, Chaeyong; Rink, Richard; Kaefer, Martin.

In: Journal of Pediatric Urology, Vol. 2, No. 4, 08.2006, p. 225-232.

Research output: Contribution to journalArticle

Lee, Sang Don ; Akbal, Cem ; Miseeri, Rosalia ; Jung, Chaeyong ; Rink, Richard ; Kaefer, Martin. / Collagen prolyl 4-hydroxylase is up-regulated in an acute bladder outlet obstruction. In: Journal of Pediatric Urology. 2006 ; Vol. 2, No. 4. pp. 225-232.
@article{ed236bf4b52d46ba828ff5123266d1be,
title = "Collagen prolyl 4-hydroxylase is up-regulated in an acute bladder outlet obstruction",
abstract = "Objective: Compliance is primarily related to extracellular matrix deposition, and prolyl 4-hydroxylase (P4Hs) plays a critical role in the synthesis of the matrix. To study the alteration of P4Hs, under the influence of variable hydrostatic pressure, a novel pressure device was used to expose human bladder smooth muscle cells (HBSMC) and fibroblasts (HBF) to pressures in the physiologic range. We then studied acute obstructed porcine bladder tissues to see if changes can also be seen after in-vitro obstruction. Materials and methods: HBSMC and HBF were exposed to pressures at 0, 20 and 40 cmH2O for up to 72 h. In-vivo studies were carried out next, using six normal (control) and five obstructed porcine bladders. Pigs were exposed to a consistent hydrostatic pressure of ≤20 cm for 24 h after ligation of the urethra. We used 2-DE to compare protein profiling of HBSMC under normal and increased pressures. Other analyses were used to detect molecular alteration and altered expression of mRNA for P4Hs. Results: We identified 437 proteins from 476 spots (91.8{\%}) obtained from HBSMC that were differentially expressed under normal and increased pressures. Under increased pressure, 48 unique proteins were significantly increased or decreased, and a prominent protein regulating extracellular matrix synthesis highly correlated with P4Hs. The exposure of both HBSMC and HBF to a sustained hydrostatic pressure resulted in the increased expression of P4Hs in a time- and pressure-dependent manner. In vivo, P4Hs expression was also significantly increased in the obstructed group. Conclusions: P4Hs is up-regulated, in the human bladder, time and pressure dependently. The alteration of P4Hs over a short period may significantly influence the synthesis of extracellular matrix in vivo and lead to decreased compliance. Our results also support the concept that bladder outlet obstruction, with resultant pressures of 40 cmH2O or less, results in molecular changes consistent with decreased compliance.",
keywords = "Bladder outlet obstruction, Bladder smooth muscle, Prolyl 4-hydroxylase",
author = "Lee, {Sang Don} and Cem Akbal and Rosalia Miseeri and Chaeyong Jung and Richard Rink and Martin Kaefer",
year = "2006",
month = "8",
doi = "10.1016/j.jpurol.2006.03.011",
language = "English",
volume = "2",
pages = "225--232",
journal = "Journal of Pediatric Urology",
issn = "1477-5131",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Collagen prolyl 4-hydroxylase is up-regulated in an acute bladder outlet obstruction

AU - Lee, Sang Don

AU - Akbal, Cem

AU - Miseeri, Rosalia

AU - Jung, Chaeyong

AU - Rink, Richard

AU - Kaefer, Martin

PY - 2006/8

Y1 - 2006/8

N2 - Objective: Compliance is primarily related to extracellular matrix deposition, and prolyl 4-hydroxylase (P4Hs) plays a critical role in the synthesis of the matrix. To study the alteration of P4Hs, under the influence of variable hydrostatic pressure, a novel pressure device was used to expose human bladder smooth muscle cells (HBSMC) and fibroblasts (HBF) to pressures in the physiologic range. We then studied acute obstructed porcine bladder tissues to see if changes can also be seen after in-vitro obstruction. Materials and methods: HBSMC and HBF were exposed to pressures at 0, 20 and 40 cmH2O for up to 72 h. In-vivo studies were carried out next, using six normal (control) and five obstructed porcine bladders. Pigs were exposed to a consistent hydrostatic pressure of ≤20 cm for 24 h after ligation of the urethra. We used 2-DE to compare protein profiling of HBSMC under normal and increased pressures. Other analyses were used to detect molecular alteration and altered expression of mRNA for P4Hs. Results: We identified 437 proteins from 476 spots (91.8%) obtained from HBSMC that were differentially expressed under normal and increased pressures. Under increased pressure, 48 unique proteins were significantly increased or decreased, and a prominent protein regulating extracellular matrix synthesis highly correlated with P4Hs. The exposure of both HBSMC and HBF to a sustained hydrostatic pressure resulted in the increased expression of P4Hs in a time- and pressure-dependent manner. In vivo, P4Hs expression was also significantly increased in the obstructed group. Conclusions: P4Hs is up-regulated, in the human bladder, time and pressure dependently. The alteration of P4Hs over a short period may significantly influence the synthesis of extracellular matrix in vivo and lead to decreased compliance. Our results also support the concept that bladder outlet obstruction, with resultant pressures of 40 cmH2O or less, results in molecular changes consistent with decreased compliance.

AB - Objective: Compliance is primarily related to extracellular matrix deposition, and prolyl 4-hydroxylase (P4Hs) plays a critical role in the synthesis of the matrix. To study the alteration of P4Hs, under the influence of variable hydrostatic pressure, a novel pressure device was used to expose human bladder smooth muscle cells (HBSMC) and fibroblasts (HBF) to pressures in the physiologic range. We then studied acute obstructed porcine bladder tissues to see if changes can also be seen after in-vitro obstruction. Materials and methods: HBSMC and HBF were exposed to pressures at 0, 20 and 40 cmH2O for up to 72 h. In-vivo studies were carried out next, using six normal (control) and five obstructed porcine bladders. Pigs were exposed to a consistent hydrostatic pressure of ≤20 cm for 24 h after ligation of the urethra. We used 2-DE to compare protein profiling of HBSMC under normal and increased pressures. Other analyses were used to detect molecular alteration and altered expression of mRNA for P4Hs. Results: We identified 437 proteins from 476 spots (91.8%) obtained from HBSMC that were differentially expressed under normal and increased pressures. Under increased pressure, 48 unique proteins were significantly increased or decreased, and a prominent protein regulating extracellular matrix synthesis highly correlated with P4Hs. The exposure of both HBSMC and HBF to a sustained hydrostatic pressure resulted in the increased expression of P4Hs in a time- and pressure-dependent manner. In vivo, P4Hs expression was also significantly increased in the obstructed group. Conclusions: P4Hs is up-regulated, in the human bladder, time and pressure dependently. The alteration of P4Hs over a short period may significantly influence the synthesis of extracellular matrix in vivo and lead to decreased compliance. Our results also support the concept that bladder outlet obstruction, with resultant pressures of 40 cmH2O or less, results in molecular changes consistent with decreased compliance.

KW - Bladder outlet obstruction

KW - Bladder smooth muscle

KW - Prolyl 4-hydroxylase

UR - http://www.scopus.com/inward/record.url?scp=33746325777&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33746325777&partnerID=8YFLogxK

U2 - 10.1016/j.jpurol.2006.03.011

DO - 10.1016/j.jpurol.2006.03.011

M3 - Article

VL - 2

SP - 225

EP - 232

JO - Journal of Pediatric Urology

JF - Journal of Pediatric Urology

SN - 1477-5131

IS - 4

ER -