Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas

Christina S. Kamma-Lorger, Craig Boote, Sally Hayes, Julie Albon, Michael E. Boulton, Keith M. Meek

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Corneal collagen ultrastructural changes occur during the healing process. The present study was designed to compare collagen ultrastructural changes after trephine wounding or flap creation. Bovine corneas were injured and maintained in organ culture for up to 4 weeks. Samples were removed from culture at 0, 1, 2, 3 and 4 weeks and snap frozen in liquid N2. X-ray scattering was used to measure changes in collagen interfibrillar spacing, intermolecular spacing and fibrillar diameter. Some samples were fixed in 10% Neutral Buffered Formalin solution and wax embedded for immunohistochemistry to monitor myofibroblast differentiation in corneal flaps. Swelling effects (i.e. changes in interfibrillar spacing) were more severe in trephined corneas than in those with stromal flaps. Collagen fibrillar diameter remained normal in the periphery of injured corneas, but increased significantly in areas within and around the wound in trephined samples and in the epithelial incision site in corneal flaps. Intermolecular spacing was unchanged in all samples. In the flaps, αSMA expression was only detected in an area adjacent to the epithelial plug, and cell numbers gradually increased during the culture. We conclude that stromal swelling is more rapid for trephine-wounded corneas than in stromal flaps, indicating that the intensity of the corneal healing response depends on the type of injury.

Original languageEnglish (US)
Pages (from-to)953-959
Number of pages7
JournalExperimental Eye Research
Volume88
Issue number5
DOIs
StatePublished - May 2009
Externally publishedYes

Fingerprint

Wound Healing
Cornea
Collagen
Fibrillar Collagens
Myofibroblasts
Waxes
Organ Culture Techniques
Wounds and Injuries
Formaldehyde
Cell Count
Epithelial Cells
Immunohistochemistry
X-Rays

Keywords

  • collagen
  • cornea
  • organ culture
  • stromal flaps
  • stromal swelling
  • trephine wounds
  • wound healing
  • X-ray scattering

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Kamma-Lorger, C. S., Boote, C., Hayes, S., Albon, J., Boulton, M. E., & Meek, K. M. (2009). Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas. Experimental Eye Research, 88(5), 953-959. https://doi.org/10.1016/j.exer.2008.12.005

Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas. / Kamma-Lorger, Christina S.; Boote, Craig; Hayes, Sally; Albon, Julie; Boulton, Michael E.; Meek, Keith M.

In: Experimental Eye Research, Vol. 88, No. 5, 05.2009, p. 953-959.

Research output: Contribution to journalArticle

Kamma-Lorger, CS, Boote, C, Hayes, S, Albon, J, Boulton, ME & Meek, KM 2009, 'Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas', Experimental Eye Research, vol. 88, no. 5, pp. 953-959. https://doi.org/10.1016/j.exer.2008.12.005
Kamma-Lorger, Christina S. ; Boote, Craig ; Hayes, Sally ; Albon, Julie ; Boulton, Michael E. ; Meek, Keith M. / Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas. In: Experimental Eye Research. 2009 ; Vol. 88, No. 5. pp. 953-959.
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