Collection and Processing of Mobilized Mouse Peripheral Blood at Lowered Oxygen Tension Yields Enhanced Numbers of Hematopoietic Stem Cells

Arafat Aljoufi, Scott Cooper, Hal E. Broxmeyer

Research output: Contribution to journalArticle

Abstract

Mobilized peripheral blood (mPB) hematopoietic stem (HSCs) and progenitor (HPCs) cells are primary sources for hematopoietic cell transplantation (HCT). Successful HCT requires threshold numbers of high-quality HSCs to reconstitute hematopoiesis long-term. Nevertheless, considerable percentages of patients and healthy donors fail to achieve required thresholds of HSCs with current mobilization regimens. In this present study we demonstrate that similar to mouse bone marrow (BM) and human cord blood, collection and processing of mouse Granulocyte Colony Stimulating Factor (G-CSF)-, AMD3100/Plerixafor- or G-CSF plus AMD3100/Plerixafor-mobilized HSCs in 3% O2 results in enhanced numbers of rigorously-defined phenotypic and for G-CSF – and G-CSF plus AMD3100/Plerixafor – mPB enhanced functionally-engrafting HSCs. These results may be of potential clinical utility. [Figure not available: see fulltext.].

Original languageEnglish (US)
Pages (from-to)946-953
Number of pages8
JournalStem Cell Reviews and Reports
Volume16
Issue number5
DOIs
StateAccepted/In press - 2020

Keywords

  • AMD3100/Plerixafor
  • G-CSF
  • Hematopoietic Stem Cell Transplantation
  • Lowered O Tension
  • Mobilized Peripheral Blood

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

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