Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use

Brandon C. Perry, Dan Zhou, Xiaohua Wu, Feng Chun Yang, Michael A. Byers, T.M. Gabriel Chu, J. Jeffrey Hockema, Erik J. Woods, W. Goebel

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

Recent studies have shown that mesenchymal stem cells (MSC) with the potential for cell-mediated therapies and tissue engineering applications can be isolated from extracted dental tissues. Here, we investigated the collection, processing, and cryobiological characteristics of MSC from human teeth processed under current good tissue practices (cGTP). Viable dental pulp-derived MSC (DPSC) cultures were isolated from 31 of 40 teeth examined. Of eight DPSC cultures examined more thoroughly, all expressed appropriate cell surface markers and underwent osteogenic, adipogenic, and chondrogenic differentiation in appropriate differentiation medium, thus meeting criteria to be called MSC. Viable DPSC were obtained up to 120 h postextraction. Efficient recovery of DPSC from cryopreserved intact teeth and second-passage DPSC cultures was achieved. These studies indicate that DPSC isolation is feasible for at least 5 days after tooth extraction, and imply that processing immediately after extraction may not be required for successful banking of DPSC. Further, the recovery of viable DPSC after cryopreservation of intact teeth suggests that minimal processing may be needed for the banking of samples with no immediate plans for expansion and use. These initial studies will facilitate the development of future cGTP protocols for the clinical banking of MSC.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalTissue Engineering - Part C: Methods
Volume14
Issue number2
DOIs
StatePublished - 2008

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Dental Pulp
Cryopreservation
Stem cells
Mesenchymal Stromal Cells
Pulp
Tooth
Tissue
Processing
Recovery
Tooth Extraction
Bioelectric potentials
Tissue Engineering
Clinical Protocols
Cell- and Tissue-Based Therapy
Tissue engineering
Cell culture
Cell Culture Techniques

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering

Cite this

Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. / Perry, Brandon C.; Zhou, Dan; Wu, Xiaohua; Yang, Feng Chun; Byers, Michael A.; Chu, T.M. Gabriel; Hockema, J. Jeffrey; Woods, Erik J.; Goebel, W.

In: Tissue Engineering - Part C: Methods, Vol. 14, No. 2, 2008, p. 149-156.

Research output: Contribution to journalArticle

Perry, Brandon C. ; Zhou, Dan ; Wu, Xiaohua ; Yang, Feng Chun ; Byers, Michael A. ; Chu, T.M. Gabriel ; Hockema, J. Jeffrey ; Woods, Erik J. ; Goebel, W. / Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use. In: Tissue Engineering - Part C: Methods. 2008 ; Vol. 14, No. 2. pp. 149-156.
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