Colocalization and coassembly of two human brain M-type potassium channel subunits that are mutated in epilepsy

Edward C. Cooper, Kenneth D. Aldape, Aviva Abosch, Nicholas Barbaro, Mitchel S. Berger, Warwick S. Peacock, Yuh Nung Jan, Lily Yeh Jan

Research output: Contribution to journalArticle

155 Citations (Scopus)

Abstract

Acetylcholine excites many central and autonomic neurons through inhibition of M-channels, slowly activating, noninactivating voltage-gated potassium channels. We here provide information regarding the in vivo distribution and biochemical characteristics of human brain KCNQ2 and KCNQ3, two channel subunits that form M-channels when expressed in vitro, and, when mutated, cause the dominantly inherited epileptic syndrome, benign neonatal familial convulsions. KCNQ2 and KCNQ3 proteins are colocalized in a somatodendritic pattern on pyramidal and polymorphic neurons in the human cortex and hippocampus. Immunoreactivity for KCNQ2, but not KCNQ3, is also prominent in some terminal fields, suggesting a presynaptic role for a distinct subgroup of M-channels in the regulation of action potential propagation and neurotransmitter release. KCNQ2 and KCNQ3 can be coimmunoprecipitated from brain lysates. Further, KCNQ2 and KCNQ3 are coassociated with tubulin and protein kinase A within a Triton X-100- insoluble protein complex. This complex is not associated with low-density membrane rafts or with N-methyl-D-aspartate receptors, PSD-95 scaffolding proteins, or other potassium channels tested. Our studies thus provide a view of a signaling complex that may be important for cognitive function as well as epilepsy. Analysis of this complex may shed light on the unknown transduction pathway linking muscarinic acetylcholine receptor activation to M-channel inhibition.

Original languageEnglish (US)
Pages (from-to)4914-4919
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number9
DOIs
StatePublished - Apr 25 2000
Externally publishedYes

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Potassium Channels
KCNQ3 Potassium Channel
KCNQ2 Potassium Channel
Epilepsy
Benign Neonatal Epilepsy
Voltage-Gated Potassium Channels
Pyramidal Cells
Octoxynol
Brain
Muscarinic Receptors
Tubulin
Cyclic AMP-Dependent Protein Kinases
N-Methyl-D-Aspartate Receptors
Cognition
Action Potentials
Acetylcholine
Neurotransmitter Agents
Hippocampus
Proteins
Neurons

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Colocalization and coassembly of two human brain M-type potassium channel subunits that are mutated in epilepsy. / Cooper, Edward C.; Aldape, Kenneth D.; Abosch, Aviva; Barbaro, Nicholas; Berger, Mitchel S.; Peacock, Warwick S.; Jan, Yuh Nung; Jan, Lily Yeh.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 9, 25.04.2000, p. 4914-4919.

Research output: Contribution to journalArticle

Cooper, Edward C. ; Aldape, Kenneth D. ; Abosch, Aviva ; Barbaro, Nicholas ; Berger, Mitchel S. ; Peacock, Warwick S. ; Jan, Yuh Nung ; Jan, Lily Yeh. / Colocalization and coassembly of two human brain M-type potassium channel subunits that are mutated in epilepsy. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 9. pp. 4914-4919.
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