Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air

Qingchun Cai, Maegan Capitano, Xinxin Huang, Bin Guo, Scott Cooper, Hal Broxmeyer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Hematopoietic cell transplantation (HCT) is a treatment for malignant and non-malignant disorders. However, sometimes the numbers of donor hematopoietic stem cells (HSC) are limiting, which can compromise the success of HCT. We recently published that collection and processing of mouse bone marrow (BM) and human cord blood cells in a hypoxic atmosphere of 3% O2 or in ambient air (∼21% O2) in the presence of cyclosporine A yields increased numbers of HSC. We now show that collection and processing of mouse BM cells in ambient air in the presence of specific combinations of anti-oxidants and/or inhibitors of epigenetic enzymes can also enhance the collection of HSC, information of potential relevance for enhanced efficacy of HCT.

Original languageEnglish (US)
JournalBlood Cells, Molecules, and Diseases
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Cell Transplantation
Enzyme Inhibitors
Hematopoietic Stem Cells
Epigenomics
Antioxidants
Bone Marrow
Air
Atmosphere
Fetal Blood
Oxidants
Bone Marrow Cells
Cyclosporine
Blood Cells
Therapeutics

Keywords

  • Antioxidants
  • Epigenetic enzyme inhibitors
  • Hematopoietic stem cell
  • Hypoxia

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

Cite this

Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air. / Cai, Qingchun; Capitano, Maegan; Huang, Xinxin; Guo, Bin; Cooper, Scott; Broxmeyer, Hal.

In: Blood Cells, Molecules, and Diseases, 01.01.2018.

Research output: Contribution to journalArticle

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AU - Cooper, Scott

AU - Broxmeyer, Hal

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