Combinations of antioxidants and/or of epigenetic enzyme inhibitors allow for enhanced collection of mouse bone marrow hematopoietic stem cells in ambient air

Qingchun Cai, Maegan Capitano, Xinxin Huang, Bin Guo, Scott Cooper, Hal E. Broxmeyer

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Hematopoietic cell transplantation (HCT) is a treatment for malignant and non-malignant disorders. However, sometimes the numbers of donor hematopoietic stem cells (HSC) are limiting, which can compromise the success of HCT. We recently published that collection and processing of mouse bone marrow (BM) and human cord blood cells in a hypoxic atmosphere of 3% O 2 or in ambient air (~21% O 2 ) in the presence of cyclosporine A yields increased numbers of HSC. We now show that collection and processing of mouse BM cells in ambient air in the presence of specific combinations of anti-oxidants and/or inhibitors of epigenetic enzymes can also enhance the collection of HSC, information of potential relevance for enhanced efficacy of HCT.

Original languageEnglish (US)
Pages (from-to)23-28
Number of pages6
JournalBlood Cells, Molecules, and Diseases
Volume71
DOIs
StatePublished - Jul 2018

Keywords

  • Antioxidants
  • Epigenetic enzyme inhibitors
  • Hematopoietic stem cell
  • Hypoxia

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Hematology
  • Cell Biology

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