Combined DOG1 and Mammaglobin Immunohistochemistry Is Comparable to ETV6-breakapart Analysis for Differentiating between Papillary Cystic Variants of Acinic Cell Carcinoma and Mammary Analogue Secretory Carcinoma

Nasser Said-Al-Naief, Roman Carlos, Gail Vance, Caroline Miller, Paul Edwards

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Background. We investigated the reliability of combined DOG1 and mammaglobin immunohistochemistry compared with ETV6 fluorescence in situ hybridization (FISH) in the assessment of salivary tumors previously diagnosed as acinic cell carcinoma (ACC). Ultrastructural features of cases reclassified as mammary analogue secretory carcinoma (MASC) were assessed by transmission electron microscopy (TEM). Methods. Immunohistochemical (IHC) reactivity to DOG1 and mammaglobin was validated against FISH targeting the ETV6 gene in all 14 cases. Results. Three cases with papillary cystic histomorphology previously diagnosed as ACC were revised to MASC. TEM features of the ETV6 rearrangement-positive MASC cases showed large numbers of secretory granules with extrusion into the intercellular spaces, well-developed endoplasmic reticulum, lipid-laden vacuoles, well-formed microvilli, and large lining cystic spaces. Conclusions. Combined DOG1 and mammaglobin immunohistochemistry is comparable to ETV6-breakapart analysis for differentiating between papillary cystic variants of ACC and MASC.

Original languageEnglish (US)
Pages (from-to)127-140
Number of pages14
JournalInternational Journal of Surgical Pathology
Volume25
Issue number2
DOIs
StatePublished - Apr 1 2017

Fingerprint

Acinar Cell Carcinoma
Immunohistochemistry
Fluorescence In Situ Hybridization
Transmission Electron Microscopy
Gene Targeting
Secretory Vesicles
Extracellular Space
Microvilli
Vacuoles
Endoplasmic Reticulum
Lipids
Mammary Analogue Secretory Carcinoma
Neoplasms

Keywords

  • acinic cell carcinoma
  • DOG1
  • ETV6
  • FISH
  • immunohistochemistry
  • mammaglobin
  • mammary analogue secretory carcinoma

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

Cite this

@article{8bb0ab30c4ff433cb6feb8dd365fbef9,
title = "Combined DOG1 and Mammaglobin Immunohistochemistry Is Comparable to ETV6-breakapart Analysis for Differentiating between Papillary Cystic Variants of Acinic Cell Carcinoma and Mammary Analogue Secretory Carcinoma",
abstract = "Background. We investigated the reliability of combined DOG1 and mammaglobin immunohistochemistry compared with ETV6 fluorescence in situ hybridization (FISH) in the assessment of salivary tumors previously diagnosed as acinic cell carcinoma (ACC). Ultrastructural features of cases reclassified as mammary analogue secretory carcinoma (MASC) were assessed by transmission electron microscopy (TEM). Methods. Immunohistochemical (IHC) reactivity to DOG1 and mammaglobin was validated against FISH targeting the ETV6 gene in all 14 cases. Results. Three cases with papillary cystic histomorphology previously diagnosed as ACC were revised to MASC. TEM features of the ETV6 rearrangement-positive MASC cases showed large numbers of secretory granules with extrusion into the intercellular spaces, well-developed endoplasmic reticulum, lipid-laden vacuoles, well-formed microvilli, and large lining cystic spaces. Conclusions. Combined DOG1 and mammaglobin immunohistochemistry is comparable to ETV6-breakapart analysis for differentiating between papillary cystic variants of ACC and MASC.",
keywords = "acinic cell carcinoma, DOG1, ETV6, FISH, immunohistochemistry, mammaglobin, mammary analogue secretory carcinoma",
author = "Nasser Said-Al-Naief and Roman Carlos and Gail Vance and Caroline Miller and Paul Edwards",
year = "2017",
month = "4",
day = "1",
doi = "10.1177/1066896916670005",
language = "English (US)",
volume = "25",
pages = "127--140",
journal = "International Journal of Surgical Pathology",
issn = "1066-8969",
publisher = "SAGE Publications Inc.",
number = "2",

}

TY - JOUR

T1 - Combined DOG1 and Mammaglobin Immunohistochemistry Is Comparable to ETV6-breakapart Analysis for Differentiating between Papillary Cystic Variants of Acinic Cell Carcinoma and Mammary Analogue Secretory Carcinoma

AU - Said-Al-Naief, Nasser

AU - Carlos, Roman

AU - Vance, Gail

AU - Miller, Caroline

AU - Edwards, Paul

PY - 2017/4/1

Y1 - 2017/4/1

N2 - Background. We investigated the reliability of combined DOG1 and mammaglobin immunohistochemistry compared with ETV6 fluorescence in situ hybridization (FISH) in the assessment of salivary tumors previously diagnosed as acinic cell carcinoma (ACC). Ultrastructural features of cases reclassified as mammary analogue secretory carcinoma (MASC) were assessed by transmission electron microscopy (TEM). Methods. Immunohistochemical (IHC) reactivity to DOG1 and mammaglobin was validated against FISH targeting the ETV6 gene in all 14 cases. Results. Three cases with papillary cystic histomorphology previously diagnosed as ACC were revised to MASC. TEM features of the ETV6 rearrangement-positive MASC cases showed large numbers of secretory granules with extrusion into the intercellular spaces, well-developed endoplasmic reticulum, lipid-laden vacuoles, well-formed microvilli, and large lining cystic spaces. Conclusions. Combined DOG1 and mammaglobin immunohistochemistry is comparable to ETV6-breakapart analysis for differentiating between papillary cystic variants of ACC and MASC.

AB - Background. We investigated the reliability of combined DOG1 and mammaglobin immunohistochemistry compared with ETV6 fluorescence in situ hybridization (FISH) in the assessment of salivary tumors previously diagnosed as acinic cell carcinoma (ACC). Ultrastructural features of cases reclassified as mammary analogue secretory carcinoma (MASC) were assessed by transmission electron microscopy (TEM). Methods. Immunohistochemical (IHC) reactivity to DOG1 and mammaglobin was validated against FISH targeting the ETV6 gene in all 14 cases. Results. Three cases with papillary cystic histomorphology previously diagnosed as ACC were revised to MASC. TEM features of the ETV6 rearrangement-positive MASC cases showed large numbers of secretory granules with extrusion into the intercellular spaces, well-developed endoplasmic reticulum, lipid-laden vacuoles, well-formed microvilli, and large lining cystic spaces. Conclusions. Combined DOG1 and mammaglobin immunohistochemistry is comparable to ETV6-breakapart analysis for differentiating between papillary cystic variants of ACC and MASC.

KW - acinic cell carcinoma

KW - DOG1

KW - ETV6

KW - FISH

KW - immunohistochemistry

KW - mammaglobin

KW - mammary analogue secretory carcinoma

UR - http://www.scopus.com/inward/record.url?scp=85014865967&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014865967&partnerID=8YFLogxK

U2 - 10.1177/1066896916670005

DO - 10.1177/1066896916670005

M3 - Article

C2 - 27670353

AN - SCOPUS:85014865967

VL - 25

SP - 127

EP - 140

JO - International Journal of Surgical Pathology

JF - International Journal of Surgical Pathology

SN - 1066-8969

IS - 2

ER -