Combined effects of dynamic tissue shear deformation and insulin-like growth factor I on chondrocyte biosynthesis in cartilage explants

Moonsoo Jin, Greg R. Emkey, Patrick Siparsky, Stephen Trippel, Alan J. Grodzinsky

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Biophysical forces and biochemical factors play crucial roles in the maintenance of the integrity of articular cartilage. In this study, we explored the effect of dynamic tissue shear deformation and insulin-like growth factor I (IGF-I) on matrix synthesis by chondrocytes within native cartilage explants. Dynamic tissue shear in the range of 0.5-6% strain amplitude at 0.1Hz was applied to cartilage explants cultured in serum-free medium. Dynamic tissue shear above 1.5% strain amplitude significantly stimulated protein and proteoglycan synthesis, by maximum values of 35 and 25%, respectively, over statically held control specimens. In the absence of tissue shear, IGF-I augmented protein and proteoglycan synthesis up to twofold at IGF-I concentrations in the range of 100-300ng/ml. When tissue shear and IGF-I stimuli were combined, matrix biosynthesis levels were significantly higher than the maximal effect caused by either stimulus alone. However, there was no significant interaction between tissue shear and IGF-I as determined by two-way ANOVA. We then quantified the effect of dynamic tissue shear on the transport of IGF-I into and within cartilage explants. [125I]IGF-I was added to the medium, and the levels of intratissue [125I]IGF-I were directly measured as a function of time over 48h in the presence and absence of continuous dynamic shear strain. Dynamic shear did not alter the rate of uptake of [125I]IGF-I into the explants, suggesting that convective diffusion of [125I]IGF-I is negligible under the shear strain conditions used. This is in marked contrast to the enhancement of transport reported in response to uniaxial dynamic compression [1]. Taken together, these data suggest that (1) the stimulatory effect of tissue shear is via mechanotransduction pathways and not by facilitated transport of biochemical factors and (2) chondrocytes may possess complementary signal transduction pathways for biophysical and biochemical factors leading to changes in metabolic activity.

Original languageEnglish
Pages (from-to)223-231
Number of pages9
JournalArchives of Biochemistry and Biophysics
Volume414
Issue number2
DOIs
StatePublished - Jun 15 2003

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Biosynthesis
Cartilage
Chondrocytes
Insulin-Like Growth Factor I
Shear deformation
Tissue
Shear strain
Proteoglycans
Signal transduction
Serum-Free Culture Media
Articular Cartilage
Analysis of variance (ANOVA)
Signal Transduction
Analysis of Variance
Proteins
Maintenance

Keywords

  • Cartilage
  • IGF-I
  • Proteoglycan
  • Tissue shear
  • Transport

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Combined effects of dynamic tissue shear deformation and insulin-like growth factor I on chondrocyte biosynthesis in cartilage explants. / Jin, Moonsoo; Emkey, Greg R.; Siparsky, Patrick; Trippel, Stephen; Grodzinsky, Alan J.

In: Archives of Biochemistry and Biophysics, Vol. 414, No. 2, 15.06.2003, p. 223-231.

Research output: Contribution to journalArticle

Jin, Moonsoo ; Emkey, Greg R. ; Siparsky, Patrick ; Trippel, Stephen ; Grodzinsky, Alan J. / Combined effects of dynamic tissue shear deformation and insulin-like growth factor I on chondrocyte biosynthesis in cartilage explants. In: Archives of Biochemistry and Biophysics. 2003 ; Vol. 414, No. 2. pp. 223-231.
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