Combined shock-wave and immunogene therapy of mouse melanoma and renal carcinoma tumors

Jianming Song, Darrell Tata, Lang Li, Jeremy Taylor, Shiping Bao, Douglas L. Miller

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The effects of ultrasonic shock waves (SW), recombinant interleukin-12 (rIL-12) protein and DNA plasmids coding for interleukin-12 (pIL-12) were investigated on progression of mouse B16 melanoma and RENCA renal carcinoma tumors. Tumor cells were implanted and grown on the hind legs of syngeneic mice. Before treatment, mice were anesthetized and the tumor region was shaved and depilated. Air bubbles at 10% of tumor volume and an equal volume of phosphate buffered saline (PBS), either with rIL-12 or pIL-12 were injected into the tumor. SW treatment consisted of 500 SWs (7.4-MPa peak negative pressure) from a spark-gap lithotripter. Tumor volume was measured every other day and tumor growth was statistically modeled. SW treatment augmented by air injection induced a tumor growth delay for a few days immediately after exposure. Intratumor rIL-12 injection enhanced the SW effect on tumor progression, to the extent that a statistically significant increase in survival was realized in both tumor models. pIL-12 injection alone, which is known to produce some gene transfer, provided no detectable tumor-growth reduction. The combination of SW and pIL-12 injection provided a statistically significant reduction in tumor growth relative to SW alone for both tumor models. IL-12 expression due to SW-induced gene transfer was confirmed in ELISA assays. This research demonstrates a potentiality for further development of ultrasound (US)-enhanced cancer gene therapy.

Original languageEnglish (US)
Pages (from-to)957-964
Number of pages8
JournalUltrasound in Medicine and Biology
Volume28
Issue number7
DOIs
StatePublished - Jul 2002
Externally publishedYes

Fingerprint

mice
shock waves
Melanoma
therapy
Shock
tumors
Interleukin-12
cancer
interleukins
Carcinoma
Kidney
Neoplasms
plasmids
Plasmids
Therapeutics
coding
Injections
injection
Growth
Tumor Burden

Keywords

  • B16 melanoma
  • Cavitation
  • Gene therapy
  • IL-12
  • Lithotripsy
  • Nonviral transfection
  • Renca
  • Sonoporation
  • Ultrasound

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Combined shock-wave and immunogene therapy of mouse melanoma and renal carcinoma tumors. / Song, Jianming; Tata, Darrell; Li, Lang; Taylor, Jeremy; Bao, Shiping; Miller, Douglas L.

In: Ultrasound in Medicine and Biology, Vol. 28, No. 7, 07.2002, p. 957-964.

Research output: Contribution to journalArticle

Song, Jianming ; Tata, Darrell ; Li, Lang ; Taylor, Jeremy ; Bao, Shiping ; Miller, Douglas L. / Combined shock-wave and immunogene therapy of mouse melanoma and renal carcinoma tumors. In: Ultrasound in Medicine and Biology. 2002 ; Vol. 28, No. 7. pp. 957-964.
@article{11ad557aa2864a08aebf2baf5400eec6,
title = "Combined shock-wave and immunogene therapy of mouse melanoma and renal carcinoma tumors",
abstract = "The effects of ultrasonic shock waves (SW), recombinant interleukin-12 (rIL-12) protein and DNA plasmids coding for interleukin-12 (pIL-12) were investigated on progression of mouse B16 melanoma and RENCA renal carcinoma tumors. Tumor cells were implanted and grown on the hind legs of syngeneic mice. Before treatment, mice were anesthetized and the tumor region was shaved and depilated. Air bubbles at 10{\%} of tumor volume and an equal volume of phosphate buffered saline (PBS), either with rIL-12 or pIL-12 were injected into the tumor. SW treatment consisted of 500 SWs (7.4-MPa peak negative pressure) from a spark-gap lithotripter. Tumor volume was measured every other day and tumor growth was statistically modeled. SW treatment augmented by air injection induced a tumor growth delay for a few days immediately after exposure. Intratumor rIL-12 injection enhanced the SW effect on tumor progression, to the extent that a statistically significant increase in survival was realized in both tumor models. pIL-12 injection alone, which is known to produce some gene transfer, provided no detectable tumor-growth reduction. The combination of SW and pIL-12 injection provided a statistically significant reduction in tumor growth relative to SW alone for both tumor models. IL-12 expression due to SW-induced gene transfer was confirmed in ELISA assays. This research demonstrates a potentiality for further development of ultrasound (US)-enhanced cancer gene therapy.",
keywords = "B16 melanoma, Cavitation, Gene therapy, IL-12, Lithotripsy, Nonviral transfection, Renca, Sonoporation, Ultrasound",
author = "Jianming Song and Darrell Tata and Lang Li and Jeremy Taylor and Shiping Bao and Miller, {Douglas L.}",
year = "2002",
month = "7",
doi = "10.1016/S0301-5629(02)00536-7",
language = "English (US)",
volume = "28",
pages = "957--964",
journal = "Ultrasound in Medicine and Biology",
issn = "0301-5629",
publisher = "Elsevier USA",
number = "7",

}

TY - JOUR

T1 - Combined shock-wave and immunogene therapy of mouse melanoma and renal carcinoma tumors

AU - Song, Jianming

AU - Tata, Darrell

AU - Li, Lang

AU - Taylor, Jeremy

AU - Bao, Shiping

AU - Miller, Douglas L.

PY - 2002/7

Y1 - 2002/7

N2 - The effects of ultrasonic shock waves (SW), recombinant interleukin-12 (rIL-12) protein and DNA plasmids coding for interleukin-12 (pIL-12) were investigated on progression of mouse B16 melanoma and RENCA renal carcinoma tumors. Tumor cells were implanted and grown on the hind legs of syngeneic mice. Before treatment, mice were anesthetized and the tumor region was shaved and depilated. Air bubbles at 10% of tumor volume and an equal volume of phosphate buffered saline (PBS), either with rIL-12 or pIL-12 were injected into the tumor. SW treatment consisted of 500 SWs (7.4-MPa peak negative pressure) from a spark-gap lithotripter. Tumor volume was measured every other day and tumor growth was statistically modeled. SW treatment augmented by air injection induced a tumor growth delay for a few days immediately after exposure. Intratumor rIL-12 injection enhanced the SW effect on tumor progression, to the extent that a statistically significant increase in survival was realized in both tumor models. pIL-12 injection alone, which is known to produce some gene transfer, provided no detectable tumor-growth reduction. The combination of SW and pIL-12 injection provided a statistically significant reduction in tumor growth relative to SW alone for both tumor models. IL-12 expression due to SW-induced gene transfer was confirmed in ELISA assays. This research demonstrates a potentiality for further development of ultrasound (US)-enhanced cancer gene therapy.

AB - The effects of ultrasonic shock waves (SW), recombinant interleukin-12 (rIL-12) protein and DNA plasmids coding for interleukin-12 (pIL-12) were investigated on progression of mouse B16 melanoma and RENCA renal carcinoma tumors. Tumor cells were implanted and grown on the hind legs of syngeneic mice. Before treatment, mice were anesthetized and the tumor region was shaved and depilated. Air bubbles at 10% of tumor volume and an equal volume of phosphate buffered saline (PBS), either with rIL-12 or pIL-12 were injected into the tumor. SW treatment consisted of 500 SWs (7.4-MPa peak negative pressure) from a spark-gap lithotripter. Tumor volume was measured every other day and tumor growth was statistically modeled. SW treatment augmented by air injection induced a tumor growth delay for a few days immediately after exposure. Intratumor rIL-12 injection enhanced the SW effect on tumor progression, to the extent that a statistically significant increase in survival was realized in both tumor models. pIL-12 injection alone, which is known to produce some gene transfer, provided no detectable tumor-growth reduction. The combination of SW and pIL-12 injection provided a statistically significant reduction in tumor growth relative to SW alone for both tumor models. IL-12 expression due to SW-induced gene transfer was confirmed in ELISA assays. This research demonstrates a potentiality for further development of ultrasound (US)-enhanced cancer gene therapy.

KW - B16 melanoma

KW - Cavitation

KW - Gene therapy

KW - IL-12

KW - Lithotripsy

KW - Nonviral transfection

KW - Renca

KW - Sonoporation

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=0036657615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036657615&partnerID=8YFLogxK

U2 - 10.1016/S0301-5629(02)00536-7

DO - 10.1016/S0301-5629(02)00536-7

M3 - Article

VL - 28

SP - 957

EP - 964

JO - Ultrasound in Medicine and Biology

JF - Ultrasound in Medicine and Biology

SN - 0301-5629

IS - 7

ER -