Comparative analysis of the influences of human gamma, alpha and beta interferons on human multipotential (CFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells

H. E. Broxmeyer, L. Lu, E. Platzer, C. Feit, L. Juliano, B. Y. Rubin

Research output: Contribution to journalArticle

277 Citations (Scopus)

Abstract

Preparations of human interferon (HulFN) immune (γ) (2 x 107 units/mg protein), HulFN leukocyte (α) (1.4 x 108 units/mg protein) and HulFN fibroblasts (β) (106 U/mg protein) were assessed for their influence on colony formation of human hematopoietic progenitor cells: colony forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), burst forming unit-erythroid (BFU-E), day 7 colony forming unit granulocyte-macrophage (CFU-GM) and day 14 CFU-GM. Colony formation by CFU-GEMM and BFU-E was suppressed equally by the three preparations of HulFN, but colony formation by CFU-GM was suppressed differentially. CFU-GM were, on the whole, more responsive to HulFNγ than HulFNα, and HulFNβ was least effective. HulFNα, but not HulFNγ or HulFNβ, suppressed colony formation from CFU-GM without also suppressing the total number of colonies plus clusters. This was due to an increase in the numbers of clusters formed in the presence of HulFNα. The suppressive influence on colonies from CFU-GM by the preoparations of HulFN and the enhancement of clusters by HulFNα was apparently equal for colonies and clusters of neutrophils, eosinophils, macrophages and neutrophils plus macrophages. The suppressive effects of HulFNγ were inactivated by a monoclonal antibody to HulFNγ and the suppressive and enhancing effects of HulFNα were inactivated with a heteroantiserum to HulFNα. Depletion of monocytes, T lymphocytes and B lymphocytes from the target bone marrow cells had no influence on the effects of the preparations of HulFN. These results demonstrate that the effects of HulFNγ and HulFNα are due to the HulFN themselves and that these actions on the hematopoietic progenitor cells are probably not mediated through monocytes and/or lymphocytes.

Original languageEnglish (US)
Pages (from-to)1300-1305
Number of pages6
JournalJournal of Immunology
Volume131
Issue number3
StatePublished - Oct 13 1983

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Myeloid Progenitor Cells
Granulocyte-Macrophage Progenitor Cells
Erythroid Precursor Cells
Interferon-beta
Interferon-alpha
Interferon-gamma
Hematopoietic Stem Cells
Monocytes
Neutrophils
Macrophages
Proteins
Eosinophils
Bone Marrow Cells
Leukocytes
B-Lymphocytes
Fibroblasts
Monoclonal Antibodies
Lymphocytes
T-Lymphocytes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Comparative analysis of the influences of human gamma, alpha and beta interferons on human multipotential (CFU-GEMM), erythroid (BFU-E) and granulocyte-macrophage (CFU-GM) progenitor cells. / Broxmeyer, H. E.; Lu, L.; Platzer, E.; Feit, C.; Juliano, L.; Rubin, B. Y.

In: Journal of Immunology, Vol. 131, No. 3, 13.10.1983, p. 1300-1305.

Research output: Contribution to journalArticle

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abstract = "Preparations of human interferon (HulFN) immune (γ) (2 x 107 units/mg protein), HulFN leukocyte (α) (1.4 x 108 units/mg protein) and HulFN fibroblasts (β) (106 U/mg protein) were assessed for their influence on colony formation of human hematopoietic progenitor cells: colony forming unit-granulocyte, erythroid, macrophage, megakaryocyte (CFU-GEMM), burst forming unit-erythroid (BFU-E), day 7 colony forming unit granulocyte-macrophage (CFU-GM) and day 14 CFU-GM. Colony formation by CFU-GEMM and BFU-E was suppressed equally by the three preparations of HulFN, but colony formation by CFU-GM was suppressed differentially. CFU-GM were, on the whole, more responsive to HulFNγ than HulFNα, and HulFNβ was least effective. HulFNα, but not HulFNγ or HulFNβ, suppressed colony formation from CFU-GM without also suppressing the total number of colonies plus clusters. This was due to an increase in the numbers of clusters formed in the presence of HulFNα. The suppressive influence on colonies from CFU-GM by the preoparations of HulFN and the enhancement of clusters by HulFNα was apparently equal for colonies and clusters of neutrophils, eosinophils, macrophages and neutrophils plus macrophages. The suppressive effects of HulFNγ were inactivated by a monoclonal antibody to HulFNγ and the suppressive and enhancing effects of HulFNα were inactivated with a heteroantiserum to HulFNα. Depletion of monocytes, T lymphocytes and B lymphocytes from the target bone marrow cells had no influence on the effects of the preparations of HulFN. These results demonstrate that the effects of HulFNγ and HulFNα are due to the HulFN themselves and that these actions on the hematopoietic progenitor cells are probably not mediated through monocytes and/or lymphocytes.",
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AU - Lu, L.

AU - Platzer, E.

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AU - Juliano, L.

AU - Rubin, B. Y.

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