Comparative anatomy of the mammalian corneal subbasal nerve plexus

Carl Marfurt, Miracle C. Anokwute, Kaleigh Fetcko, Erin Mahony-Perez, Hassan Farooq, Emily Ross, Maraya M. Baumanis, Rachel L. Weinberg, Megan E. McCarron, Joseph L. Mankowski

Research output: Contribution to journalArticle

Abstract

PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.

Original languageEnglish (US)
Pages (from-to)4972-4984
Number of pages13
JournalInvestigative Ophthalmology and Visual Science
Volume60
Issue number15
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

Fingerprint

Comparative Anatomy
Cornea
Nerve Fibers
Macaca
Guinea Pigs
Photomicrography
Limbus Corneae
Dogs
Rabbits
Sus scrofa
Nose
Immune Sera
Mammals
Swine
Animal Models
Organizations
Light
Research

Keywords

  • Corneal innervation
  • Corneal nerves
  • Ocular nerves
  • Subbasal nerves
  • Trigeminal nerve

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Marfurt, C., Anokwute, M. C., Fetcko, K., Mahony-Perez, E., Farooq, H., Ross, E., ... Mankowski, J. L. (2019). Comparative anatomy of the mammalian corneal subbasal nerve plexus. Investigative Ophthalmology and Visual Science, 60(15), 4972-4984. https://doi.org/10.1167/iovs.19-28519

Comparative anatomy of the mammalian corneal subbasal nerve plexus. / Marfurt, Carl; Anokwute, Miracle C.; Fetcko, Kaleigh; Mahony-Perez, Erin; Farooq, Hassan; Ross, Emily; Baumanis, Maraya M.; Weinberg, Rachel L.; McCarron, Megan E.; Mankowski, Joseph L.

In: Investigative Ophthalmology and Visual Science, Vol. 60, No. 15, 01.01.2019, p. 4972-4984.

Research output: Contribution to journalArticle

Marfurt, C, Anokwute, MC, Fetcko, K, Mahony-Perez, E, Farooq, H, Ross, E, Baumanis, MM, Weinberg, RL, McCarron, ME & Mankowski, JL 2019, 'Comparative anatomy of the mammalian corneal subbasal nerve plexus', Investigative Ophthalmology and Visual Science, vol. 60, no. 15, pp. 4972-4984. https://doi.org/10.1167/iovs.19-28519
Marfurt, Carl ; Anokwute, Miracle C. ; Fetcko, Kaleigh ; Mahony-Perez, Erin ; Farooq, Hassan ; Ross, Emily ; Baumanis, Maraya M. ; Weinberg, Rachel L. ; McCarron, Megan E. ; Mankowski, Joseph L. / Comparative anatomy of the mammalian corneal subbasal nerve plexus. In: Investigative Ophthalmology and Visual Science. 2019 ; Vol. 60, No. 15. pp. 4972-4984.
@article{40bf2aa680574498b313afc03924f903,
title = "Comparative anatomy of the mammalian corneal subbasal nerve plexus",
abstract = "PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.",
keywords = "Corneal innervation, Corneal nerves, Ocular nerves, Subbasal nerves, Trigeminal nerve",
author = "Carl Marfurt and Anokwute, {Miracle C.} and Kaleigh Fetcko and Erin Mahony-Perez and Hassan Farooq and Emily Ross and Baumanis, {Maraya M.} and Weinberg, {Rachel L.} and McCarron, {Megan E.} and Mankowski, {Joseph L.}",
year = "2019",
month = "1",
day = "1",
doi = "10.1167/iovs.19-28519",
language = "English (US)",
volume = "60",
pages = "4972--4984",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "15",

}

TY - JOUR

T1 - Comparative anatomy of the mammalian corneal subbasal nerve plexus

AU - Marfurt, Carl

AU - Anokwute, Miracle C.

AU - Fetcko, Kaleigh

AU - Mahony-Perez, Erin

AU - Farooq, Hassan

AU - Ross, Emily

AU - Baumanis, Maraya M.

AU - Weinberg, Rachel L.

AU - McCarron, Megan E.

AU - Mankowski, Joseph L.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.

AB - PURPOSE: The subbasal nerve plexus (SNP) is the densest and most recognizable component of the mammalian corneal innervation; however, the anatomical configuration of the SNP in most animal models remains incompletely described. The purpose of the current study is to describe in detail the SNP architecture in eight different mammals, including several popular animal models used in cornea research. METHODS: Corneal nerves in mouse, rat, guinea pig, rabbit, dog, macaque, domestic pig, and cow eyes were stained immunohistochemically with antiserum directed against neurotubulin. SNP architecture was documented by digital photomicrography and large-scale reconstructions, that is, corneal nerve maps, using a drawing tube attached to a light microscope. RESULTS: Subbasal nerve fibers (SNFs) in mice, rats, guinea pigs, dogs, and macaques radiated centrally from the corneoscleral limbus toward the corneal apex in a whorl-like or spiraling pattern. SNFs in rabbit and bovine corneas swept horizontally across the ocular surface in a temporal-to-nasal direction and converged on the inferonasal limbus without forming a spiral. SNFs in the pig cornea radiated centrifugally in all directions, like a starburst, from a focal point located equidistant between the corneal apex and the superior pole. CONCLUSIONS: The results of the present study have demonstrated for the first time substantial interspecies differences in the architectural organization of the mammalian SNP. The physiological significance of these different patterns and the mechanisms that regulate SNP pattern formation in the mammalian cornea remain incompletely understood and warrant additional investigation.

KW - Corneal innervation

KW - Corneal nerves

KW - Ocular nerves

KW - Subbasal nerves

KW - Trigeminal nerve

UR - http://www.scopus.com/inward/record.url?scp=85075916919&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85075916919&partnerID=8YFLogxK

U2 - 10.1167/iovs.19-28519

DO - 10.1167/iovs.19-28519

M3 - Article

C2 - 31790560

AN - SCOPUS:85075916919

VL - 60

SP - 4972

EP - 4984

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 15

ER -