Comparative Effect of Two Red Lights on Streptococcus mutans Biofilms and Assessment of Temperature Variances in Human Teeth During In Vitro Photodynamic Antimicrobial Chemotherapy

Maria Gerusa Brito Aragão, Cecília Atem Gonçalves de Araújo Costa, Ramile Araújo Lima, Lidiany Karla Azevedo Rodrigues, Simone Duarte, Iriana Carla Junqueira Zanin

Research output: Contribution to journalArticle

Abstract

Objective: The goals of this investigation were to compare the effect of photodynamic antimicrobial chemotherapy (PACT) with two different red lights on in vitro Streptococcus mutans biofilms, as well as to assess the temperature variances caused by PACT on human teeth. Methods:S. mutans biofilms (n = 3) were grown on hydroxyapatite disks, and the antimicrobial effect of PACT was evaluated using toluidine blue O (100 μg/mL) associated with Laserbeam® (LB 56.6 J/cm2) and LumaCare™ (LC -56.6, 158.5, 317.0, and 475.6 J/cm2). Pulpal temperature variances were analyzed using a digital thermocouple placed into the pulp chamber and positioned at the cement-enamel junction level of five teeth samples during irradiation times of 300, 600, and 900 sec for LB, and 22, 60, 120, and 180 sec for LC. The mean average temperature variance was calculated for each group. All data were analyzed through analysis of variance. Results: LB (900 sec) and LC (22 sec) induced similar reductions in the viability of microorganisms. LB did not cause statistically significant increase of temperature, regardless of experimental time, and LC caused temperature increase within the safe spectrum up to 60 sec. Conclusions: PACT seems to be a minimal invasive approach for reducing the viability of cariogenic bacteria. Thus, when applied in vitro for times equal or inferior to 900 and 60 sec for LB and LC, respectively, these light sources might be considered harmless to tooth structures.

Original languageEnglish (US)
Pages (from-to)31-37
Number of pages7
JournalPhotobiomodulation, photomedicine, and laser surgery
Volume37
Issue number1
DOIs
StatePublished - Jan 1 2019
Externally publishedYes

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Streptococcus mutans
Chemotherapy
Biofilms
Tooth
Light
Drug Therapy
Temperature
Microbial Viability
Tolonium Chloride
Enamels
Dental Pulp Cavity
Durapatite
Dental Enamel
Analysis of variance (ANOVA)
Thermocouples
Hydroxyapatite
Microorganisms
Pulp
Light sources
Bacteria

Keywords

  • anti-infective agents
  • biofilms
  • dental pulp cavity
  • photochemotherapy
  • temperature

Cite this

Comparative Effect of Two Red Lights on Streptococcus mutans Biofilms and Assessment of Temperature Variances in Human Teeth During In Vitro Photodynamic Antimicrobial Chemotherapy. / Aragão, Maria Gerusa Brito; Costa, Cecília Atem Gonçalves de Araújo; Lima, Ramile Araújo; Rodrigues, Lidiany Karla Azevedo; Duarte, Simone; Zanin, Iriana Carla Junqueira.

In: Photobiomodulation, photomedicine, and laser surgery, Vol. 37, No. 1, 01.01.2019, p. 31-37.

Research output: Contribution to journalArticle

Aragão, Maria Gerusa Brito ; Costa, Cecília Atem Gonçalves de Araújo ; Lima, Ramile Araújo ; Rodrigues, Lidiany Karla Azevedo ; Duarte, Simone ; Zanin, Iriana Carla Junqueira. / Comparative Effect of Two Red Lights on Streptococcus mutans Biofilms and Assessment of Temperature Variances in Human Teeth During In Vitro Photodynamic Antimicrobial Chemotherapy. In: Photobiomodulation, photomedicine, and laser surgery. 2019 ; Vol. 37, No. 1. pp. 31-37.
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AU - Costa, Cecília Atem Gonçalves de Araújo

AU - Lima, Ramile Araújo

AU - Rodrigues, Lidiany Karla Azevedo

AU - Duarte, Simone

AU - Zanin, Iriana Carla Junqueira

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N2 - Objective: The goals of this investigation were to compare the effect of photodynamic antimicrobial chemotherapy (PACT) with two different red lights on in vitro Streptococcus mutans biofilms, as well as to assess the temperature variances caused by PACT on human teeth. Methods:S. mutans biofilms (n = 3) were grown on hydroxyapatite disks, and the antimicrobial effect of PACT was evaluated using toluidine blue O (100 μg/mL) associated with Laserbeam® (LB 56.6 J/cm2) and LumaCare™ (LC -56.6, 158.5, 317.0, and 475.6 J/cm2). Pulpal temperature variances were analyzed using a digital thermocouple placed into the pulp chamber and positioned at the cement-enamel junction level of five teeth samples during irradiation times of 300, 600, and 900 sec for LB, and 22, 60, 120, and 180 sec for LC. The mean average temperature variance was calculated for each group. All data were analyzed through analysis of variance. Results: LB (900 sec) and LC (22 sec) induced similar reductions in the viability of microorganisms. LB did not cause statistically significant increase of temperature, regardless of experimental time, and LC caused temperature increase within the safe spectrum up to 60 sec. Conclusions: PACT seems to be a minimal invasive approach for reducing the viability of cariogenic bacteria. Thus, when applied in vitro for times equal or inferior to 900 and 60 sec for LB and LC, respectively, these light sources might be considered harmless to tooth structures.

AB - Objective: The goals of this investigation were to compare the effect of photodynamic antimicrobial chemotherapy (PACT) with two different red lights on in vitro Streptococcus mutans biofilms, as well as to assess the temperature variances caused by PACT on human teeth. Methods:S. mutans biofilms (n = 3) were grown on hydroxyapatite disks, and the antimicrobial effect of PACT was evaluated using toluidine blue O (100 μg/mL) associated with Laserbeam® (LB 56.6 J/cm2) and LumaCare™ (LC -56.6, 158.5, 317.0, and 475.6 J/cm2). Pulpal temperature variances were analyzed using a digital thermocouple placed into the pulp chamber and positioned at the cement-enamel junction level of five teeth samples during irradiation times of 300, 600, and 900 sec for LB, and 22, 60, 120, and 180 sec for LC. The mean average temperature variance was calculated for each group. All data were analyzed through analysis of variance. Results: LB (900 sec) and LC (22 sec) induced similar reductions in the viability of microorganisms. LB did not cause statistically significant increase of temperature, regardless of experimental time, and LC caused temperature increase within the safe spectrum up to 60 sec. Conclusions: PACT seems to be a minimal invasive approach for reducing the viability of cariogenic bacteria. Thus, when applied in vitro for times equal or inferior to 900 and 60 sec for LB and LC, respectively, these light sources might be considered harmless to tooth structures.

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KW - dental pulp cavity

KW - photochemotherapy

KW - temperature

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