Comparative effects in vivo of recombinant murine interleukin 3, natural murine colony-stimulating factor-1, and recombinant murine granulocyte-macrophage colony-stimulating factor on myelopoiesis in mice

Hal Broxmeyer, D. E. Williams, S. Cooper, R. K. Shadduck, S. Gillis, A. Waheed, D. L. Urdal, D. C. Bicknell

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Abstract

Purified murine colony-stimulating factors (CSF) recombinant interleukin 3 (IL-3), natural CSF-1, and recombinant granulocyte-macrophage (GM) CSF were assessed in vivo for their effects on BDF1 mouse bone marrow and spleen granulocyte-macrophage (CFU-GM), erythroid (BFU-E), and multipotential (CFU-GEMM) progenitor cells in untreated mice and mice pretreated with purified iron-saturated human lactoferrin (LF). The CSF and LF preparations did not contain detectable endotoxin (< 0.1 ng). Mice pretreated with LF were more sensitive to the effects of CSF. In mice pretreated with LF, 2,000 U IL-3 or 20,000 U CSF-1 significantly enhanced the cycling status and absolute numbers of all progenitors, whereas 20,000 U GM-CSF significantly increased the cycling status of CFU-GM and CFU-GEMM, but had no effect on cycling of BFU-E or on numbers of any of the progenitors. The effects of CSF in mice pretreated with LF were not mimicked by 0.1-100 ng E. coli lipopolysaccharide.

Original languageEnglish
Pages (from-to)721-730
Number of pages10
JournalJournal of Clinical Investigation
Volume79
Issue number3
StatePublished - 1987

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Myelopoiesis
Macrophage Colony-Stimulating Factor
Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Lactoferrin
Colony-Stimulating Factors
Granulocytes
Myeloid Progenitor Cells
Erythroid Precursor Cells
Macrophages
Endotoxins
Lipopolysaccharides
Stem Cells
Spleen
Iron
Bone Marrow
Escherichia coli

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Comparative effects in vivo of recombinant murine interleukin 3, natural murine colony-stimulating factor-1, and recombinant murine granulocyte-macrophage colony-stimulating factor on myelopoiesis in mice. / Broxmeyer, Hal; Williams, D. E.; Cooper, S.; Shadduck, R. K.; Gillis, S.; Waheed, A.; Urdal, D. L.; Bicknell, D. C.

In: Journal of Clinical Investigation, Vol. 79, No. 3, 1987, p. 721-730.

Research output: Contribution to journalArticle

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AU - Broxmeyer, Hal

AU - Williams, D. E.

AU - Cooper, S.

AU - Shadduck, R. K.

AU - Gillis, S.

AU - Waheed, A.

AU - Urdal, D. L.

AU - Bicknell, D. C.

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N2 - Purified murine colony-stimulating factors (CSF) recombinant interleukin 3 (IL-3), natural CSF-1, and recombinant granulocyte-macrophage (GM) CSF were assessed in vivo for their effects on BDF1 mouse bone marrow and spleen granulocyte-macrophage (CFU-GM), erythroid (BFU-E), and multipotential (CFU-GEMM) progenitor cells in untreated mice and mice pretreated with purified iron-saturated human lactoferrin (LF). The CSF and LF preparations did not contain detectable endotoxin (< 0.1 ng). Mice pretreated with LF were more sensitive to the effects of CSF. In mice pretreated with LF, 2,000 U IL-3 or 20,000 U CSF-1 significantly enhanced the cycling status and absolute numbers of all progenitors, whereas 20,000 U GM-CSF significantly increased the cycling status of CFU-GM and CFU-GEMM, but had no effect on cycling of BFU-E or on numbers of any of the progenitors. The effects of CSF in mice pretreated with LF were not mimicked by 0.1-100 ng E. coli lipopolysaccharide.

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