Comparative proteomic profiles of meningioma subtypes

Hiroaki Okamoto, Jie Li, Alexander O. Vortmeyer, Howard Jaffe, Youn Soo Lee, Sven Gläsker, Tae Sung Sohn, Weifen Zeng, Barbara Ikejiri, Martin A. Proescholdt, Christina Mayer, Robert J. Weil, Edward H. Oldfield, Zhengping Zhuang

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Meningiomas are classified into three groups (benign, atypical, and anaplastic) based on morphologic characteristics. Atypical meningiomas, which are WHO grade 2 tumors, and anaplastic meningiomas, which are WHO grade 3 tumors, exhibit an increased risk of recurrence and premature death compared with benign WHO grade 1 tumors. Although atypical and anaplastic meningiomas account for <10% of all of meningiomas, it can be difficult to distinguish them from benign meningiomas by morphologic criteria alone. We used selective tissue microdissection to examine 24 human meningiomas and did two-dimensional gel electrophoresis to determine protein expression patterns. Proteins expressed differentially by meningiomas of each WHO grade were identified and sequenced. Proteomic analysis revealed protein expression patterns unique to WHO grade 1, 2, and 3 meningiomas and identified 24 proteins that distinguish each subtype. Fifteen proteins showed significant changes in expression level between benign and atypical meningiomas, whereas nine distinguished atypical from anaplastic meningiomas. Differential protein expression was confirmed by Western blotting and immunohistochemistry. We established differential proteomic profiles that characterize and distinguish meningiomas of increasing grades. The proteins and proteomic profiles enhance understanding of the pathogenesis of meningiomas and have implications for diagnosis, prognosis, and treatment.

Original languageEnglish (US)
Pages (from-to)10199-10204
Number of pages6
JournalCancer Research
Volume66
Issue number20
DOIs
StatePublished - Oct 15 2006
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Okamoto, H., Li, J., Vortmeyer, A. O., Jaffe, H., Lee, Y. S., Gläsker, S., Sohn, T. S., Zeng, W., Ikejiri, B., Proescholdt, M. A., Mayer, C., Weil, R. J., Oldfield, E. H., & Zhuang, Z. (2006). Comparative proteomic profiles of meningioma subtypes. Cancer Research, 66(20), 10199-10204. https://doi.org/10.1158/0008-5472.CAN-06-0955