Comparative study of biphasic calcium phosphate ceramics impregnated with rhBMP-2 as bone substitutes

Md Imranul Alam, Izumi Asahina, Kohichi Ohmamiuda, Shoji Enomoto

Research output: Contribution to journalArticle

93 Scopus citations


We investigated pellet-shaped implants prepared from biphasic calcium phosphate (BCP) ceramics with five different ratios of hydroxyapatite (HAP) to β-tricalcium phosphate (β-TCP) to evaluate these ceramics as bone substitutes. BCP ceramics impregnated with different doses of recombinant human bone morphogenetic protein 2 (rhBMP-2) (1, 5, and 10 μg) were used for experimental purposes and ceramics without rhBMP-2 were used for control. The pellets were implanted under the pericranium in adult Wistar male rats and were harvested 8 weeks after implantation. The retrieved pellets were then examined radiologically, histologically, and histomorphometrically. The results revealed that the pellets treated with rhBMP-2 exhibited new bone and bone marrow, whereas control pellets produced fibrous connective tissues. The formation of new bone induced by rhBMP-2 was dose dependent. The extent of bone and bone marrow formation and the degree of resorption of the ceramic particles were significantly higher in the pellets composed of 25% HAP-75% TCP. In this study, bioresorption of the ceramic produced favorable conditions for rhBMP-2-induced bone formation. (C) 2000 John Wiley and Sons, Inc.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalJournal of Biomedical Materials Research
Issue number1
StatePublished - Jan 1 2001
Externally publishedYes


  • Biphasic calcium phosphate
  • Bone morphogenetic protein
  • Bone substitute
  • HAP/TCP pellet
  • New bone formation

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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