Comparative study of phase II biotransformation in rabbit ocular tissues

J. B. Watkins, D. P. Wirthwein, R. A. Sanders

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

To assess the biotransformational capability of ocular tissues in the rabbit, representative phase II enzymes were assayed in five tissues from the eye, and in the liver, kidney, and intestine. Within the eye, the iris/ciliary body exhibited the highest glutathione S-transferase activity, whereas the cornea possessed the highest specific activities for N-acetyl-, sulfo-, and UDP-glucuronosyl-transferases. Cornea, iris/ciliary body, choroid, and retina exhibited significant activities of p-aminobenzoic acid N-acetyltransferase, 2-naphthol sulfo-transferase, and 1-chloro-2,4-dinitrobenzene glutathione S-transferase. Despite its size and protein content, lens displayed little or no biotransformational activity. Only the iris/ciliary body conjugated sulfobromophthalein with glutathione. UDP-glucuronosyltransferase activity varied depending on tested substrates and tissues. When compared to liver, kidney, or intestine, N-acetyltransferase activity in the iris/ciliary body nearly matched the rate measured in kidney, glutathione S-transferase activity in cornea and iris/ciliary body was nearly 70 and 89%, respectively, of the rate in intestine, and corneal sulfotransferase activity was greater than that in kidney. These data suggest that biotransformation pathways are present in the eye, and particularly in ocular tissues having adequate blood supply or interfacing with the external environment.

Original languageEnglish
Pages (from-to)708-713
Number of pages6
JournalDrug Metabolism and Disposition
Volume19
Issue number3
StatePublished - 1991

Fingerprint

Ciliary Body
Iris
Biotransformation
Glutathione Transferase
Tissue
Rabbits
Acetyltransferases
Cornea
Intestines
Kidney
Transferases
Liver
Sulfobromophthalein
4-Aminobenzoic Acid
Sulfotransferases
Dinitrochlorobenzene
Glucuronosyltransferase
Crystallins
Uridine Diphosphate
Choroid

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Watkins, J. B., Wirthwein, D. P., & Sanders, R. A. (1991). Comparative study of phase II biotransformation in rabbit ocular tissues. Drug Metabolism and Disposition, 19(3), 708-713.

Comparative study of phase II biotransformation in rabbit ocular tissues. / Watkins, J. B.; Wirthwein, D. P.; Sanders, R. A.

In: Drug Metabolism and Disposition, Vol. 19, No. 3, 1991, p. 708-713.

Research output: Contribution to journalArticle

Watkins, JB, Wirthwein, DP & Sanders, RA 1991, 'Comparative study of phase II biotransformation in rabbit ocular tissues', Drug Metabolism and Disposition, vol. 19, no. 3, pp. 708-713.
Watkins, J. B. ; Wirthwein, D. P. ; Sanders, R. A. / Comparative study of phase II biotransformation in rabbit ocular tissues. In: Drug Metabolism and Disposition. 1991 ; Vol. 19, No. 3. pp. 708-713.
@article{4f0c45699a7c430097d6f74f9067cd8d,
title = "Comparative study of phase II biotransformation in rabbit ocular tissues",
abstract = "To assess the biotransformational capability of ocular tissues in the rabbit, representative phase II enzymes were assayed in five tissues from the eye, and in the liver, kidney, and intestine. Within the eye, the iris/ciliary body exhibited the highest glutathione S-transferase activity, whereas the cornea possessed the highest specific activities for N-acetyl-, sulfo-, and UDP-glucuronosyl-transferases. Cornea, iris/ciliary body, choroid, and retina exhibited significant activities of p-aminobenzoic acid N-acetyltransferase, 2-naphthol sulfo-transferase, and 1-chloro-2,4-dinitrobenzene glutathione S-transferase. Despite its size and protein content, lens displayed little or no biotransformational activity. Only the iris/ciliary body conjugated sulfobromophthalein with glutathione. UDP-glucuronosyltransferase activity varied depending on tested substrates and tissues. When compared to liver, kidney, or intestine, N-acetyltransferase activity in the iris/ciliary body nearly matched the rate measured in kidney, glutathione S-transferase activity in cornea and iris/ciliary body was nearly 70 and 89{\%}, respectively, of the rate in intestine, and corneal sulfotransferase activity was greater than that in kidney. These data suggest that biotransformation pathways are present in the eye, and particularly in ocular tissues having adequate blood supply or interfacing with the external environment.",
author = "Watkins, {J. B.} and Wirthwein, {D. P.} and Sanders, {R. A.}",
year = "1991",
language = "English",
volume = "19",
pages = "708--713",
journal = "Drug Metabolism and Disposition",
issn = "0090-9556",
publisher = "American Society for Pharmacology and Experimental Therapeutics",
number = "3",

}

TY - JOUR

T1 - Comparative study of phase II biotransformation in rabbit ocular tissues

AU - Watkins, J. B.

AU - Wirthwein, D. P.

AU - Sanders, R. A.

PY - 1991

Y1 - 1991

N2 - To assess the biotransformational capability of ocular tissues in the rabbit, representative phase II enzymes were assayed in five tissues from the eye, and in the liver, kidney, and intestine. Within the eye, the iris/ciliary body exhibited the highest glutathione S-transferase activity, whereas the cornea possessed the highest specific activities for N-acetyl-, sulfo-, and UDP-glucuronosyl-transferases. Cornea, iris/ciliary body, choroid, and retina exhibited significant activities of p-aminobenzoic acid N-acetyltransferase, 2-naphthol sulfo-transferase, and 1-chloro-2,4-dinitrobenzene glutathione S-transferase. Despite its size and protein content, lens displayed little or no biotransformational activity. Only the iris/ciliary body conjugated sulfobromophthalein with glutathione. UDP-glucuronosyltransferase activity varied depending on tested substrates and tissues. When compared to liver, kidney, or intestine, N-acetyltransferase activity in the iris/ciliary body nearly matched the rate measured in kidney, glutathione S-transferase activity in cornea and iris/ciliary body was nearly 70 and 89%, respectively, of the rate in intestine, and corneal sulfotransferase activity was greater than that in kidney. These data suggest that biotransformation pathways are present in the eye, and particularly in ocular tissues having adequate blood supply or interfacing with the external environment.

AB - To assess the biotransformational capability of ocular tissues in the rabbit, representative phase II enzymes were assayed in five tissues from the eye, and in the liver, kidney, and intestine. Within the eye, the iris/ciliary body exhibited the highest glutathione S-transferase activity, whereas the cornea possessed the highest specific activities for N-acetyl-, sulfo-, and UDP-glucuronosyl-transferases. Cornea, iris/ciliary body, choroid, and retina exhibited significant activities of p-aminobenzoic acid N-acetyltransferase, 2-naphthol sulfo-transferase, and 1-chloro-2,4-dinitrobenzene glutathione S-transferase. Despite its size and protein content, lens displayed little or no biotransformational activity. Only the iris/ciliary body conjugated sulfobromophthalein with glutathione. UDP-glucuronosyltransferase activity varied depending on tested substrates and tissues. When compared to liver, kidney, or intestine, N-acetyltransferase activity in the iris/ciliary body nearly matched the rate measured in kidney, glutathione S-transferase activity in cornea and iris/ciliary body was nearly 70 and 89%, respectively, of the rate in intestine, and corneal sulfotransferase activity was greater than that in kidney. These data suggest that biotransformation pathways are present in the eye, and particularly in ocular tissues having adequate blood supply or interfacing with the external environment.

UR - http://www.scopus.com/inward/record.url?scp=0025818473&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025818473&partnerID=8YFLogxK

M3 - Article

C2 - 1680641

AN - SCOPUS:0025818473

VL - 19

SP - 708

EP - 713

JO - Drug Metabolism and Disposition

JF - Drug Metabolism and Disposition

SN - 0090-9556

IS - 3

ER -