Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice

Payal B. Watchmaker, Katharina Lahl, Mike Lee, Dirk Baumjohann, John Morton, Sun Jung Kim, Ruizhu Zeng, Alexander Dent, K. Mark Ansel, Betty Diamond, Husein Hadeiba, Eugene C. Butcher

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterized human gut DC populations and defined their relationship to previously studied human and mouse DCs. CD103 +Sirpα- DCs were related to human blood CD141 + DCs and to mouse intestinal CD103+CD11b- DCs and expressed markers of cross-presenting DCs. CD103+Sirpα + DCs aligned with human blood CD1c+ DCs and mouse intestinal CD103+CD11b+ DCs and supported the induction of regulatory T cells. Both CD103+ DC subsets induced the T H17 subset of helper T cells, while CD103 -Sirpα+ DCs induced the TH1 subset of helper T cells. Comparative analysis of transcriptomes revealed conserved transcriptional programs among CD103+ DC subsets and identified a selective role for the transcriptional repressors Bcl-6 and Blimp-1 in the specification of CD103+CD11b- DCs and intestinal CD103+CD11b+ DCs, respectively. Our results highlight evolutionarily conserved and divergent programming of intestinal DCs.

Original languageEnglish
Pages (from-to)98-108
Number of pages11
JournalNature Immunology
Volume15
Issue number1
DOIs
StatePublished - Jan 2014

Fingerprint

Dendritic Cells
Cell Differentiation
Helper-Inducer T-Lymphocytes
Mucosal Immunity
Gene Expression Profiling
Regulatory T-Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Watchmaker, P. B., Lahl, K., Lee, M., Baumjohann, D., Morton, J., Kim, S. J., ... Butcher, E. C. (2014). Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice. Nature Immunology, 15(1), 98-108. https://doi.org/10.1038/ni.2768

Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice. / Watchmaker, Payal B.; Lahl, Katharina; Lee, Mike; Baumjohann, Dirk; Morton, John; Kim, Sun Jung; Zeng, Ruizhu; Dent, Alexander; Ansel, K. Mark; Diamond, Betty; Hadeiba, Husein; Butcher, Eugene C.

In: Nature Immunology, Vol. 15, No. 1, 01.2014, p. 98-108.

Research output: Contribution to journalArticle

Watchmaker, PB, Lahl, K, Lee, M, Baumjohann, D, Morton, J, Kim, SJ, Zeng, R, Dent, A, Ansel, KM, Diamond, B, Hadeiba, H & Butcher, EC 2014, 'Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice', Nature Immunology, vol. 15, no. 1, pp. 98-108. https://doi.org/10.1038/ni.2768
Watchmaker, Payal B. ; Lahl, Katharina ; Lee, Mike ; Baumjohann, Dirk ; Morton, John ; Kim, Sun Jung ; Zeng, Ruizhu ; Dent, Alexander ; Ansel, K. Mark ; Diamond, Betty ; Hadeiba, Husein ; Butcher, Eugene C. / Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice. In: Nature Immunology. 2014 ; Vol. 15, No. 1. pp. 98-108.
@article{087b4ac3c48f43a18298270284f199d9,
title = "Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice",
abstract = "Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterized human gut DC populations and defined their relationship to previously studied human and mouse DCs. CD103 +Sirpα- DCs were related to human blood CD141 + DCs and to mouse intestinal CD103+CD11b- DCs and expressed markers of cross-presenting DCs. CD103+Sirpα + DCs aligned with human blood CD1c+ DCs and mouse intestinal CD103+CD11b+ DCs and supported the induction of regulatory T cells. Both CD103+ DC subsets induced the T H17 subset of helper T cells, while CD103 -Sirpα+ DCs induced the TH1 subset of helper T cells. Comparative analysis of transcriptomes revealed conserved transcriptional programs among CD103+ DC subsets and identified a selective role for the transcriptional repressors Bcl-6 and Blimp-1 in the specification of CD103+CD11b- DCs and intestinal CD103+CD11b+ DCs, respectively. Our results highlight evolutionarily conserved and divergent programming of intestinal DCs.",
author = "Watchmaker, {Payal B.} and Katharina Lahl and Mike Lee and Dirk Baumjohann and John Morton and Kim, {Sun Jung} and Ruizhu Zeng and Alexander Dent and Ansel, {K. Mark} and Betty Diamond and Husein Hadeiba and Butcher, {Eugene C.}",
year = "2014",
month = "1",
doi = "10.1038/ni.2768",
language = "English",
volume = "15",
pages = "98--108",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Comparative transcriptional and functional profiling defines conserved programs of intestinal DC differentiation in humans and mice

AU - Watchmaker, Payal B.

AU - Lahl, Katharina

AU - Lee, Mike

AU - Baumjohann, Dirk

AU - Morton, John

AU - Kim, Sun Jung

AU - Zeng, Ruizhu

AU - Dent, Alexander

AU - Ansel, K. Mark

AU - Diamond, Betty

AU - Hadeiba, Husein

AU - Butcher, Eugene C.

PY - 2014/1

Y1 - 2014/1

N2 - Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterized human gut DC populations and defined their relationship to previously studied human and mouse DCs. CD103 +Sirpα- DCs were related to human blood CD141 + DCs and to mouse intestinal CD103+CD11b- DCs and expressed markers of cross-presenting DCs. CD103+Sirpα + DCs aligned with human blood CD1c+ DCs and mouse intestinal CD103+CD11b+ DCs and supported the induction of regulatory T cells. Both CD103+ DC subsets induced the T H17 subset of helper T cells, while CD103 -Sirpα+ DCs induced the TH1 subset of helper T cells. Comparative analysis of transcriptomes revealed conserved transcriptional programs among CD103+ DC subsets and identified a selective role for the transcriptional repressors Bcl-6 and Blimp-1 in the specification of CD103+CD11b- DCs and intestinal CD103+CD11b+ DCs, respectively. Our results highlight evolutionarily conserved and divergent programming of intestinal DCs.

AB - Dendritic cells (DCs) that orchestrate mucosal immunity have been studied in mice. Here we characterized human gut DC populations and defined their relationship to previously studied human and mouse DCs. CD103 +Sirpα- DCs were related to human blood CD141 + DCs and to mouse intestinal CD103+CD11b- DCs and expressed markers of cross-presenting DCs. CD103+Sirpα + DCs aligned with human blood CD1c+ DCs and mouse intestinal CD103+CD11b+ DCs and supported the induction of regulatory T cells. Both CD103+ DC subsets induced the T H17 subset of helper T cells, while CD103 -Sirpα+ DCs induced the TH1 subset of helper T cells. Comparative analysis of transcriptomes revealed conserved transcriptional programs among CD103+ DC subsets and identified a selective role for the transcriptional repressors Bcl-6 and Blimp-1 in the specification of CD103+CD11b- DCs and intestinal CD103+CD11b+ DCs, respectively. Our results highlight evolutionarily conserved and divergent programming of intestinal DCs.

UR - http://www.scopus.com/inward/record.url?scp=84890987173&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84890987173&partnerID=8YFLogxK

U2 - 10.1038/ni.2768

DO - 10.1038/ni.2768

M3 - Article

VL - 15

SP - 98

EP - 108

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 1

ER -