Comparing cardiac action potentials recorded with metal and glass microelectrodes

Chikaya Omichi, Moon Hyoung Lee, Toshihiko Ohara, Ajay M. Naik, Nina C. Wang, Hrayr S. Karagueuzian, Peng-Sheng Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Machine-pulled high-impedance glass capillary microelectrode is standard for transmembrane potential (TMP) recordings. However, it is fragile and difficult to impale, especially in beating myocardial tissues. We hypothesize that a high-impedance pure iridium metal electrode can be used as an alternative to the glass microelectrode for TMP recording. The TMPs were simultaneously recorded from isolated perfused swine right ventricles with a metal microelectrode and a standard glass microelectrode during pacing and during ventricular fibrillation. The basic morphology of TMP recorded with these electrodes was comparable. The action potential duration (APD) at 90% repolarization was 241 ± 29 ms for the metal microelectrode and 236 ± 31 ms for the glass microelectrode with a good correlation (r = 0.99, P <0.0001). The maximum slope value of the APD restitution curves during pacing was also significantly correlated. One metal microelectrode and >20 glass microelectrodes were needed per study. We conclude that, in isolated perfused swine right ventricles, the TMP recorded by the metal microelectrode is comparable with that recorded by the glass microelectrode. Because the metal microelectrode is more durable than the glass microelectrode, it can serve as an alternative for APD recording and for restitution analyses.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume279
Issue number6 48-6
StatePublished - 2000
Externally publishedYes

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Microelectrodes
Action Potentials
Glass
Metals
Membrane Potentials
Electric Impedance
Heart Ventricles
Electrodes
Swine
Thymidine Monophosphate
Iridium
Ventricular Fibrillation

Keywords

  • Action potential duration restitution curve
  • Transmembrane action potential
  • Ventricular fibrillation

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Comparing cardiac action potentials recorded with metal and glass microelectrodes. / Omichi, Chikaya; Lee, Moon Hyoung; Ohara, Toshihiko; Naik, Ajay M.; Wang, Nina C.; Karagueuzian, Hrayr S.; Chen, Peng-Sheng.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 279, No. 6 48-6, 2000.

Research output: Contribution to journalArticle

Omichi, Chikaya ; Lee, Moon Hyoung ; Ohara, Toshihiko ; Naik, Ajay M. ; Wang, Nina C. ; Karagueuzian, Hrayr S. ; Chen, Peng-Sheng. / Comparing cardiac action potentials recorded with metal and glass microelectrodes. In: American Journal of Physiology - Heart and Circulatory Physiology. 2000 ; Vol. 279, No. 6 48-6.
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AU - Karagueuzian, Hrayr S.

AU - Chen, Peng-Sheng

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