Comparison of 4 commercially available group B Streptococcus molecular assays using remnant rectal–vaginal enrichment broths

Research output: Contribution to journalArticle

Abstract

The incidence of neonatal Group B streptococcal (GBS) disease has significantly declined since the widespread implementation of prenatal screening of expectant mothers for urogenital and gastrointestinal tract GBS colonization. Screening methods have evolved from exclusively culture-based approaches to more rapid and highly sensitive molecular methods. We chose to evaluate the performance of 4 commercially available GBS molecular tests for detection of GBS colonization using 299 antepartum rectal–vaginal specimens submitted to our laboratory for routine GBS screening. In 97% of instances, there was agreement between all 3 systems. When testing 1, 6, and 12 samples simultaneously, all methods performed comparably, but the ARIES® GBS assay required the least total hands-on time and the illumigene® Group B Streptococcus assay required the most hands-on time.

Original languageEnglish (US)
JournalDiagnostic Microbiology and Infectious Disease
DOIs
StateAccepted/In press - Jan 1 2018

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Streptococcus agalactiae
Prenatal Diagnosis
Gastrointestinal Tract
Incidence

Keywords

  • Group B Streptococcus
  • Neonatal meningitis
  • Neonatal sepsis
  • Real-time PCR
  • Streptococcus agalactiae

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

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title = "Comparison of 4 commercially available group B Streptococcus molecular assays using remnant rectal–vaginal enrichment broths",
abstract = "The incidence of neonatal Group B streptococcal (GBS) disease has significantly declined since the widespread implementation of prenatal screening of expectant mothers for urogenital and gastrointestinal tract GBS colonization. Screening methods have evolved from exclusively culture-based approaches to more rapid and highly sensitive molecular methods. We chose to evaluate the performance of 4 commercially available GBS molecular tests for detection of GBS colonization using 299 antepartum rectal–vaginal specimens submitted to our laboratory for routine GBS screening. In 97{\%} of instances, there was agreement between all 3 systems. When testing 1, 6, and 12 samples simultaneously, all methods performed comparably, but the ARIES{\circledR} GBS assay required the least total hands-on time and the illumigene{\circledR} Group B Streptococcus assay required the most hands-on time.",
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