Comparison of a 550,000 dalton cartilage matrix glycoprotein in cartilage from immature and mature dogs

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Abstract

Cartilage matrix glycoprotein is a disulfide bonded 550,000 dalton protein found in cartilage and in the vitreous of the eye. Immunofluorescence studies using a specific antiserum to this glycoprotein have previously demonstrated a difference between the topographic distribution of staining for cartilage matrix glycoprotein in cartilage from immature and mature animals. Using a polyclonal antiserum to cartilage matrix glycoprotein, we studied this protein cartilage from normal immature and mature dogs to determine if it changes with maturation. Cartilage matrix glycoprotein from immature canine cartilage migrates as a doublet, with apparent molecular weights of 100-116,000, regardless of its state of reduction, and is immunologically cross reactive with cartilage matrix glycoprotein from adult canine cartilage. Cartilage matrix glycoprotein from mature canine cartilage is disulfide bonded before reduction with 2-mercaptoethanol and its 116,000 Da subunit migrates as a single band after reduction.

Original languageEnglish (US)
Pages (from-to)656-659
Number of pages4
JournalJournal of Rheumatology
Volume16
Issue number5
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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