Comparison of alterations in tyrosine hydroxylase, dopamine levels, and dopamine uptake in the striatum of the weaver mutant mouse

J. A. Richter, E. H. Stotz, Bernardino Ghetti, J. R. Simon

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Previous reports have shown that among the markers for the nigro-striatal dopamine (DA) system measured in the striatum, dopamine uptake seems to be more severely affected than the others in the weaver mutant mouse. In the present study we examined DA levels, tyrosine hydroxylase (TH) activity, and high-affinity DA uptake to determine if the DA uptake is most affected when all the measurements are made in the same striatal homogenate in the same laboratory. We found that the DA uptake activity was most altered (93% lower) compared to DA levels (68% lower) and TH activity (64% lower). The DA uptake was so low in the weaver that we could not obtain reliable kinetic parameters. For TH activity we found that the Vmax was 36% lower while the Km for l-tyrosine was 92% higher in the weaver striatum. This lower affinity for substrate suggests that the TH enzyme itself may be altered in the nigro-striatal system of the weaver mutant mouse.

Original languageEnglish
Pages (from-to)437-441
Number of pages5
JournalNeurochemical Research
Volume17
Issue number5
DOIs
StatePublished - May 1992

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Neurologic Mutant Mice
Tyrosine 3-Monooxygenase
Dopamine
Corpus Striatum
Kinetic parameters
Tyrosine

Keywords

  • Dopamine
  • mutant mice
  • nigrostriatal pathway
  • tyrosine hydroxylase
  • weaver

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Comparison of alterations in tyrosine hydroxylase, dopamine levels, and dopamine uptake in the striatum of the weaver mutant mouse. / Richter, J. A.; Stotz, E. H.; Ghetti, Bernardino; Simon, J. R.

In: Neurochemical Research, Vol. 17, No. 5, 05.1992, p. 437-441.

Research output: Contribution to journalArticle

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