Comparison of an Enzyme Immunoassay Versus Mass Spectrometry-based Assay for the Quantitative Determination of the Procollagen Type I N-terminal Propeptide in Rat Serum

Monika Dzieciatkowska, Marci Copeland, Jinsam You, Jean Pierre Wery, Mu Wang

Research output: Contribution to journalArticle

Abstract

Traditionally, antibody-based assays, such as enzyme immunoassay (EIA), enzyme-linked immunosorbent assay (ELISA)and radioimmunoassay (RIA), are the primary tool for the targeted quantification of a specific protein. An antibody-based assay can berun at high-throughput and has extraordinary sensitivity and specificity. In the cases where antibody-based assays exist, the processof validating biomarker candidates can be relatively straightforward. However, the antibody-based approach is limited by the lack ofavailability of antibodies with high specificity. The development of a high quality antibody-based assays can be costly, time-consumingand a resource-intensive effort. Another disadvantage of antibody-based assays is that they often do not discriminate closely relatedisoforms. While the antibody development is central to the success of antibody-based platform, mass spectrometry (MS) providesalternative and complementary approach to existing antibody-based assays. The MS-based assays are becoming very popular forquantitative candidates proteins detection in a complex biological mixture.In the present paper, an in-house developed mass spectrometry (MS)-based assay was compared to a commercially available EIA inreproducibility, measurement accuracy, and dynamic range using rat procollagen type-I N-terminal propeptide (P1NP) as a model.

Original languageEnglish
Pages (from-to)33-38
Number of pages6
JournalProteomics Insights
Volume2
Issue number1
StatePublished - 2009

Fingerprint

Collagen Type I
Immunoenzyme Techniques
Mass spectrometry
Rats
Assays
Mass Spectrometry
Antibodies
Enzymes
Serum
Immunosorbents
Biomarkers
Complex Mixtures
Radioimmunoassay
Proteins
Enzyme-Linked Immunosorbent Assay
Throughput
Sensitivity and Specificity

Keywords

  • Immunoassay
  • Mass spectrometry
  • Procollagen type-I N-terminal propeptide
  • Selected-reaction-monitoring

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry

Cite this

Comparison of an Enzyme Immunoassay Versus Mass Spectrometry-based Assay for the Quantitative Determination of the Procollagen Type I N-terminal Propeptide in Rat Serum. / Dzieciatkowska, Monika; Copeland, Marci; You, Jinsam; Wery, Jean Pierre; Wang, Mu.

In: Proteomics Insights, Vol. 2, No. 1, 2009, p. 33-38.

Research output: Contribution to journalArticle

@article{48976ec6577949909815097660f8ee0d,
title = "Comparison of an Enzyme Immunoassay Versus Mass Spectrometry-based Assay for the Quantitative Determination of the Procollagen Type I N-terminal Propeptide in Rat Serum",
abstract = "Traditionally, antibody-based assays, such as enzyme immunoassay (EIA), enzyme-linked immunosorbent assay (ELISA)and radioimmunoassay (RIA), are the primary tool for the targeted quantification of a specific protein. An antibody-based assay can berun at high-throughput and has extraordinary sensitivity and specificity. In the cases where antibody-based assays exist, the processof validating biomarker candidates can be relatively straightforward. However, the antibody-based approach is limited by the lack ofavailability of antibodies with high specificity. The development of a high quality antibody-based assays can be costly, time-consumingand a resource-intensive effort. Another disadvantage of antibody-based assays is that they often do not discriminate closely relatedisoforms. While the antibody development is central to the success of antibody-based platform, mass spectrometry (MS) providesalternative and complementary approach to existing antibody-based assays. The MS-based assays are becoming very popular forquantitative candidates proteins detection in a complex biological mixture.In the present paper, an in-house developed mass spectrometry (MS)-based assay was compared to a commercially available EIA inreproducibility, measurement accuracy, and dynamic range using rat procollagen type-I N-terminal propeptide (P1NP) as a model.",
keywords = "Immunoassay, Mass spectrometry, Procollagen type-I N-terminal propeptide, Selected-reaction-monitoring",
author = "Monika Dzieciatkowska and Marci Copeland and Jinsam You and Wery, {Jean Pierre} and Mu Wang",
year = "2009",
language = "English",
volume = "2",
pages = "33--38",
journal = "Proteomics Insights",
issn = "1178-6418",
publisher = "Libertas Academica Ltd.",
number = "1",

}

TY - JOUR

T1 - Comparison of an Enzyme Immunoassay Versus Mass Spectrometry-based Assay for the Quantitative Determination of the Procollagen Type I N-terminal Propeptide in Rat Serum

AU - Dzieciatkowska, Monika

AU - Copeland, Marci

AU - You, Jinsam

AU - Wery, Jean Pierre

AU - Wang, Mu

PY - 2009

Y1 - 2009

N2 - Traditionally, antibody-based assays, such as enzyme immunoassay (EIA), enzyme-linked immunosorbent assay (ELISA)and radioimmunoassay (RIA), are the primary tool for the targeted quantification of a specific protein. An antibody-based assay can berun at high-throughput and has extraordinary sensitivity and specificity. In the cases where antibody-based assays exist, the processof validating biomarker candidates can be relatively straightforward. However, the antibody-based approach is limited by the lack ofavailability of antibodies with high specificity. The development of a high quality antibody-based assays can be costly, time-consumingand a resource-intensive effort. Another disadvantage of antibody-based assays is that they often do not discriminate closely relatedisoforms. While the antibody development is central to the success of antibody-based platform, mass spectrometry (MS) providesalternative and complementary approach to existing antibody-based assays. The MS-based assays are becoming very popular forquantitative candidates proteins detection in a complex biological mixture.In the present paper, an in-house developed mass spectrometry (MS)-based assay was compared to a commercially available EIA inreproducibility, measurement accuracy, and dynamic range using rat procollagen type-I N-terminal propeptide (P1NP) as a model.

AB - Traditionally, antibody-based assays, such as enzyme immunoassay (EIA), enzyme-linked immunosorbent assay (ELISA)and radioimmunoassay (RIA), are the primary tool for the targeted quantification of a specific protein. An antibody-based assay can berun at high-throughput and has extraordinary sensitivity and specificity. In the cases where antibody-based assays exist, the processof validating biomarker candidates can be relatively straightforward. However, the antibody-based approach is limited by the lack ofavailability of antibodies with high specificity. The development of a high quality antibody-based assays can be costly, time-consumingand a resource-intensive effort. Another disadvantage of antibody-based assays is that they often do not discriminate closely relatedisoforms. While the antibody development is central to the success of antibody-based platform, mass spectrometry (MS) providesalternative and complementary approach to existing antibody-based assays. The MS-based assays are becoming very popular forquantitative candidates proteins detection in a complex biological mixture.In the present paper, an in-house developed mass spectrometry (MS)-based assay was compared to a commercially available EIA inreproducibility, measurement accuracy, and dynamic range using rat procollagen type-I N-terminal propeptide (P1NP) as a model.

KW - Immunoassay

KW - Mass spectrometry

KW - Procollagen type-I N-terminal propeptide

KW - Selected-reaction-monitoring

UR - http://www.scopus.com/inward/record.url?scp=84865135680&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84865135680&partnerID=8YFLogxK

M3 - Article

VL - 2

SP - 33

EP - 38

JO - Proteomics Insights

JF - Proteomics Insights

SN - 1178-6418

IS - 1

ER -