Comparison of biomarker expression between proximal and distal colorectal adenomas: The Tennessee-Indiana Adenoma Recurrence Study

Timothy Su, M. Kay Washington, Reid M. Ness, Douglas Rex, Walter E. Smalley, Thomas Ulbright, Qiuyin Cai, Wei Zheng, Martha J. Shrubsole

Research output: Contribution to journalArticle

Abstract

It is unclear if proximal and distal traditional adenomas present with differences in molecular events which contribute to cancer heterogeneity by tumor anatomical subsite. Participants from a colonoscopy-based study (n=380) were divided into subgroups based on the location of their most advanced adenoma: proximal, distal, or "equivalent both sides." Eight biomarkers in the most advanced adenomas were evaluated by immunohistochemistry (Ki-67, COX-2, TGFβRII, EGFR, β-catenin, cyclin D1, c-Myc) or TUNEL (apoptosis). After an adjustment for pathological features, there were no significant differences between proximal and distal adenomas for any biomarker. Conversely, expression levels did vary by other features, such as their size, villous component, and synchronousness. Large adenomas had higher expression levels of Ki-67(P<0.001), TGFβRII (P<0.0001), c-Myc (P<0.001), and cyclin D1 (P<0.001) in comparison to small adenomas, and tubulovillous/villous adenomas also were more likely to have similar higher expression levels in comparison to tubular adenomas. Adenoma location is not a major determinant of the expression of these biomarkers outside of other pathological features. This study suggests similarly important roles of Wnt/β-catenin and TGF-β pathways in carcinogenesis in both the proximal and distal colorectum.

Original languageEnglish (US)
JournalMolecular Carcinogenesis
DOIs
StateAccepted/In press - 2016

Fingerprint

Adenoma
Biomarkers
Recurrence
Catenins
Cyclin D1
Villous Adenoma
In Situ Nick-End Labeling
Colonoscopy
Neoplasms
Carcinogenesis
Immunohistochemistry
Apoptosis

Keywords

  • Biomarkers
  • Colorectal adenoma
  • Distal
  • Immunohistochemistry
  • Proximal
  • β-catenin

ASJC Scopus subject areas

  • Medicine(all)
  • Molecular Biology
  • Cancer Research

Cite this

Comparison of biomarker expression between proximal and distal colorectal adenomas : The Tennessee-Indiana Adenoma Recurrence Study. / Su, Timothy; Washington, M. Kay; Ness, Reid M.; Rex, Douglas; Smalley, Walter E.; Ulbright, Thomas; Cai, Qiuyin; Zheng, Wei; Shrubsole, Martha J.

In: Molecular Carcinogenesis, 2016.

Research output: Contribution to journalArticle

Su, Timothy ; Washington, M. Kay ; Ness, Reid M. ; Rex, Douglas ; Smalley, Walter E. ; Ulbright, Thomas ; Cai, Qiuyin ; Zheng, Wei ; Shrubsole, Martha J. / Comparison of biomarker expression between proximal and distal colorectal adenomas : The Tennessee-Indiana Adenoma Recurrence Study. In: Molecular Carcinogenesis. 2016.
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