Comparison of enzyme immunoassays for antibodies to Haemophilus ducreyi in a community outbreak of chancroid in the United States

Cheng Yen Chen, Kristen J. Mertz, Stanley Spinola, Stephen A. Morse

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The performance of two EIAs (adsorption EIA and lipooligosaccharide [LOS] EIA) that detect antibodies to Haemophilus ducreyi was evaluated with serum specimens obtained from 163 patients (96 with genital ulcer disease [GUD]). Paired serum specimens (initial and follow-up) were obtained from 52 of the GUD patients. By use of initial serum specimens from 82 GUD patients whose etiologic agents for their ulcers had been identified, the adsorption EIA had a sensitivity and specificity for chancroid of 53% and 71%, while the LOS EIA had a sensitivity and specificity of 48% and 89%, respectively. Sensitivity and specificity of the adsorption EIA increased to 78% and 84%, respectively, when the results of follow-up serum specimens were used to calculate optimal performance. The proportion of patients testing positive for H. ducreyi who had anti-H. ducreyi IgG antibodies, as determined by adsorption EIA, increased with the duration of infection, thus limiting the role of EIAs in the diagnosis of chancroid.

Original languageEnglish (US)
Pages (from-to)1390-1395
Number of pages6
JournalJournal of Infectious Diseases
Volume175
Issue number6
StatePublished - 1997
Externally publishedYes

Fingerprint

Haemophilus ducreyi
Chancroid
Immunoenzyme Techniques
Adsorption
Ulcer
Disease Outbreaks
Antibodies
Serum
Sensitivity and Specificity
Immunoglobulin G
Infection

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

Cite this

Comparison of enzyme immunoassays for antibodies to Haemophilus ducreyi in a community outbreak of chancroid in the United States. / Chen, Cheng Yen; Mertz, Kristen J.; Spinola, Stanley; Morse, Stephen A.

In: Journal of Infectious Diseases, Vol. 175, No. 6, 1997, p. 1390-1395.

Research output: Contribution to journalArticle

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