Comparison of growth, neovascularization, and enzymatic function of fetal intestinal grafts in the omentum and renal capsule

Karen Tisinai, Frederick Shedd, Robert Harris, Joseph Unthank, Jeffrey Grosfeld, Khalil Abu-Dalu, Jay Grosfeld

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Fetal tissues are less immunogenic and may be a useful donor source for organ transplantation. This report compares the fate of fetal small bowel segments transplanted in the omentum and renal capsule of recipient syngeneic rats. Two-centimeter segments of fetal jejunum and ileum were obtained from 26 donor 19-day gestational age rat fetuses and transplanted into the subrenal capsule (n = 35) and omentum (n = 40) in syngeneic Fisher rats (weight, 150 g) as free grafts. No immunosuppression was used. At 2 weeks posttransplantation, the recipient rats underwent laparotomy and the grafts were evaluated for viability, growth, enzymatic function, and revascularization. Viable grafts were identified in 27 of 35 renal capsule grafts and 34 of 40 omental grafts. The order of magnitude of fetal growth in the omentum for jejunum was 16 ± 10 versus ileum 23 ± 9 (NS). However, in the renal capsule, ileal growth (15 ± 6) was significantly greater than jejunum (8 ± 5; P < .01). Growth for both jejunal and ileal segments was greater in the omentum (P < .02). The lumen of all omental grafts remained patent; however, 26 of 27 renal grafts had cystic dilatations and areas of obstruction. Microfil casts of the specimens showed vascular connections (neovascularization) between the graft and omentum, a normal serosal vascular pattern, and many submucosal capillary-like vessels. Maltase activity was measured in fetal grafts and compared with control pups bred on the same date as the donor animals. The grafts had a higher maltase level 33.4 ± 34.6 μmol/min/g than controls 8.3 ± 2.0 (P < .005). Maltase activity between control jejunum and ileum was similar (7.7 ± 1.5 v 9.0 ± 2.3 [NS]). Fetal jejunal specimens (51.2 ± 41.9) had greater maltase activity than ileum (15.6 ± 6.7; P < .005). Maltase in fetal ileum was similar in omentum and renal capsule; however, jejunal maltase was significantly higher in the renal site (85.0 ± 45.7) versus omentum (25.0 ± 10.0; P < .02). These data indicate that fetal intestinal grafts can be successfully (78% to 86%) implanted in an intraabdominal site in syngeneic recipients. Growth of fetal grafts, neovascularity, and enzymatic function (jejunum > ileum) were documented in both the renal capsule and omentum; however, bowel morphology and luminal patency was better in the omentum. These observations suggest that free-fetal bowel grafts may have a potential role in bowel transplantation.

Original languageEnglish (US)
Pages (from-to)914-916
Number of pages3
JournalJournal of pediatric surgery
Volume25
Issue number8
DOIs
StatePublished - Aug 1990

Keywords

  • fetal intestinal grafts
  • Small bowel transplantation

ASJC Scopus subject areas

  • Surgery

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