Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats

Zachary Rodd, Richard Bell, Roberto I. Melendez, Kelly A. Kuc, Lawrence Lumeng, Ting Kai Li, James M. Murphy, William J. McBride

Research output: Contribution to journalArticle

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Abstract

Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the ventral tegmental area (VTA), whereas the alcohol-nonpreferring line did not. Wistar rats will also self-administer EtOH directly into the posterior VTA. Because Wistar rats also have a low preference for EtOH solutions but self-inject EtOH into the VTA, this study was undertaken to test the hypothesis that there is an association between EtOH preference and sensitivity of the VTA to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that received one of the following concentrations of EtOH: 0,50, 75,100,150, or 200 mg/100 ml. Rats were connected to the microinjection system, placed into two-lever (active and inactive) experimental chambers, and given EtOH for the first four sessions (acquisition), artificial cerebrospinal fluid for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: P rats self-infused 75 to 200 mg/100 ml EtOH and demonstrated lever discrimination, whereas Wistar rats reliably self-infused only 150 and 200 mg/100 ml EtOH. Both P and Wistar rats reduced responding on the active lever when artificial cerebrospinal fluid (aCSF) was substituted for EtOH and reinstated responding in session 7 when EtOH was restored, although P rats demonstrated a very robust enhancement of responding for 100 and 150 mg/100 ml EtOH, and this was not found for Wistar rats. Conclusions: These results suggest that, compared with Wistar rats, the posterior VTA of P rats was more sensitive to the reinforcing effects of EtOH. Furthermore, the reinstatement data suggest that the posterior VTA of P rats underwent neuronal alterations as a result of prior EtOH exposure and extinction that changed the reinforcing effects of EtOH within this region.

Original languageEnglish
Pages (from-to)1212-1219
Number of pages8
JournalAlcoholism: Clinical and Experimental Research
Volume28
Issue number8
DOIs
StatePublished - Aug 2004

Fingerprint

Ventral Tegmental Area
Self Administration
Wistar Rats
Rats
Ethanol
Alcohols
Cerebrospinal Fluid
Cerebrospinal fluid
Microinjections
Injections

Keywords

  • Alcohol-Preferring Rats
  • Ethanol
  • Intracranial Self-Administration
  • Reinforcement
  • Ventral Tegmental Area

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats. / Rodd, Zachary; Bell, Richard; Melendez, Roberto I.; Kuc, Kelly A.; Lumeng, Lawrence; Li, Ting Kai; Murphy, James M.; McBride, William J.

In: Alcoholism: Clinical and Experimental Research, Vol. 28, No. 8, 08.2004, p. 1212-1219.

Research output: Contribution to journalArticle

Rodd, Zachary ; Bell, Richard ; Melendez, Roberto I. ; Kuc, Kelly A. ; Lumeng, Lawrence ; Li, Ting Kai ; Murphy, James M. ; McBride, William J. / Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats. In: Alcoholism: Clinical and Experimental Research. 2004 ; Vol. 28, No. 8. pp. 1212-1219.
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T1 - Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats

AU - Rodd, Zachary

AU - Bell, Richard

AU - Melendez, Roberto I.

AU - Kuc, Kelly A.

AU - Lumeng, Lawrence

AU - Li, Ting Kai

AU - Murphy, James M.

AU - McBride, William J.

PY - 2004/8

Y1 - 2004/8

N2 - Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the ventral tegmental area (VTA), whereas the alcohol-nonpreferring line did not. Wistar rats will also self-administer EtOH directly into the posterior VTA. Because Wistar rats also have a low preference for EtOH solutions but self-inject EtOH into the VTA, this study was undertaken to test the hypothesis that there is an association between EtOH preference and sensitivity of the VTA to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that received one of the following concentrations of EtOH: 0,50, 75,100,150, or 200 mg/100 ml. Rats were connected to the microinjection system, placed into two-lever (active and inactive) experimental chambers, and given EtOH for the first four sessions (acquisition), artificial cerebrospinal fluid for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: P rats self-infused 75 to 200 mg/100 ml EtOH and demonstrated lever discrimination, whereas Wistar rats reliably self-infused only 150 and 200 mg/100 ml EtOH. Both P and Wistar rats reduced responding on the active lever when artificial cerebrospinal fluid (aCSF) was substituted for EtOH and reinstated responding in session 7 when EtOH was restored, although P rats demonstrated a very robust enhancement of responding for 100 and 150 mg/100 ml EtOH, and this was not found for Wistar rats. Conclusions: These results suggest that, compared with Wistar rats, the posterior VTA of P rats was more sensitive to the reinforcing effects of EtOH. Furthermore, the reinstatement data suggest that the posterior VTA of P rats underwent neuronal alterations as a result of prior EtOH exposure and extinction that changed the reinforcing effects of EtOH within this region.

AB - Background: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the ventral tegmental area (VTA), whereas the alcohol-nonpreferring line did not. Wistar rats will also self-administer EtOH directly into the posterior VTA. Because Wistar rats also have a low preference for EtOH solutions but self-inject EtOH into the VTA, this study was undertaken to test the hypothesis that there is an association between EtOH preference and sensitivity of the VTA to the reinforcing effects of EtOH. Methods: Adult P and Wistar rats were assigned to groups that received one of the following concentrations of EtOH: 0,50, 75,100,150, or 200 mg/100 ml. Rats were connected to the microinjection system, placed into two-lever (active and inactive) experimental chambers, and given EtOH for the first four sessions (acquisition), artificial cerebrospinal fluid for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. Results: P rats self-infused 75 to 200 mg/100 ml EtOH and demonstrated lever discrimination, whereas Wistar rats reliably self-infused only 150 and 200 mg/100 ml EtOH. Both P and Wistar rats reduced responding on the active lever when artificial cerebrospinal fluid (aCSF) was substituted for EtOH and reinstated responding in session 7 when EtOH was restored, although P rats demonstrated a very robust enhancement of responding for 100 and 150 mg/100 ml EtOH, and this was not found for Wistar rats. Conclusions: These results suggest that, compared with Wistar rats, the posterior VTA of P rats was more sensitive to the reinforcing effects of EtOH. Furthermore, the reinstatement data suggest that the posterior VTA of P rats underwent neuronal alterations as a result of prior EtOH exposure and extinction that changed the reinforcing effects of EtOH within this region.

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KW - Intracranial Self-Administration

KW - Reinforcement

KW - Ventral Tegmental Area

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