Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis

Giuseppe Del Priore, Robert Chatterton, Chung Lee, Richard Silver, Lori Berg, Men Jean Lee

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Two dimensional electrophoresis is a technique whereby protein sources are organized by molecular weight and electrical charge on a Polyacrylamide gel. To detect changes that may be associated with the onset of parturition, we compared electrophoresis patterns of mononuclear cell proteins and plasma proteins before and during active labor. Mononuclear cells and plasma were obtained from 5 pregnant volunteers. It appears that A-1 apolipoprotein, identified by Western Blot analysis, is increased in mononuclear cells during active labor. Conversely, a decrease in the concentration of A-1 apolipoprotein was seen in plasma during parturition. These “normal” protein patterns can now be compared with those observed in pathologic conditions including preterm labor and may allow early detection of disease by protein abnormalities before clinical signs and symptoms develop.

Original languageEnglish (US)
Pages (from-to)373-377
Number of pages5
JournalJournal of Perinatal Medicine
Volume19
Issue number5
DOIs
StatePublished - Jan 1991

Keywords

  • Geburt
  • Leukocytes
  • Leukozyten
  • Plasma
  • parturition
  • plasma
  • two dimensional Polyacrylamide gel electrophoresis
  • zweidimensionale Polyacrylamid-Gelelektrophorese

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynecology

Fingerprint Dive into the research topics of 'Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis'. Together they form a unique fingerprint.

  • Cite this