Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis

G. Del Priore, R. J. Chatterton, C. Lee, R. Silver, L. Berg, M. J. Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Two dimensional electrophoresis is a technique whereby protein sources are organized by molecular weight and electrical charge on a polyacrylamide gel. To detect changes that may be associated with the onset of parturition, we compared electrophoresis patterns of mononuclear cell proteins and plasma proteins before and during active labor. Mononuclear cells and plasma were obtained from 5 pregnant volunteers. It appears that A-1 apolipoprotein, identified by Western Blot analysis, is increased in mononuclear cells during active labor. Conversely, a decrease in the concentration of A-1 apolipoprotein was seen in plasma during parturition. These 'normal' protein patterns can now be compared with those observed in pathologic conditions including preterm labor and may allow early detection of discase by protein abnormalities before clinical signs and symptoms develop.

Original languageEnglish (US)
Pages (from-to)373-377
Number of pages5
JournalJournal of Perinatal Medicine
Volume19
Issue number5
StatePublished - 1991
Externally publishedYes

Fingerprint

Electrophoresis
Blood Proteins
Parturition
Apolipoprotein A-I
Proteins
Chymopapain
Premature Obstetric Labor
Plasma Cells
Signs and Symptoms
Volunteers
Molecular Weight
Western Blotting

Keywords

  • Leukocytes
  • Parturition
  • Plasma
  • Two dimensional polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Pediatrics, Perinatology, and Child Health

Cite this

Del Priore, G., Chatterton, R. J., Lee, C., Silver, R., Berg, L., & Lee, M. J. (1991). Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis. Journal of Perinatal Medicine, 19(5), 373-377.

Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis. / Del Priore, G.; Chatterton, R. J.; Lee, C.; Silver, R.; Berg, L.; Lee, M. J.

In: Journal of Perinatal Medicine, Vol. 19, No. 5, 1991, p. 373-377.

Research output: Contribution to journalArticle

Del Priore, G, Chatterton, RJ, Lee, C, Silver, R, Berg, L & Lee, MJ 1991, 'Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis', Journal of Perinatal Medicine, vol. 19, no. 5, pp. 373-377.
Del Priore, G. ; Chatterton, R. J. ; Lee, C. ; Silver, R. ; Berg, L. ; Lee, M. J. / Comparison of mononuclear cell proteins and plasma proteins before and during parturition by two dimensional electrophoresis. In: Journal of Perinatal Medicine. 1991 ; Vol. 19, No. 5. pp. 373-377.
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