Comparison of p53 immunoexpression with allelic loss of p53 in ulcerative colitis-associated dysplasia and carcinoma

Franz Fogt, Zengping Zhuang, Christopher Poremba, Barbara Dockhorn-Dworniczak, Alexander Vortmeyer

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We correlated p53 overexpression with allelic deletion of p53 in ulcerative colitis (UC) with high grade dysplasia (HGD, n=12) and carcinoma (CA, n=8). Sections were immunostained against p53 and epithelium was microdissected on consecutive sections with subsequent amplification for LOH of p53 (17p). Staining with anti-p53 was positive in HGD (9 of 12) and CA (7 of 8). Percent positive cells were less in HGD than in CA. LOH of p53 was present in HGD (5 of 12) and CA (5 of 7). Of cases with <10% of positive cells, including negative cases, 50% also showed LOH. These results suggest that most cases with prominent p53 overexpression but also significant numbers of cases with weak or negative expression have associated allelic p53 deletion. We conclude that i) immunohistochemical stains but not LOH for p53 correlate with progression of dysplasia to carcinoma, ii) p53 immunohistochemistry appears to more accurately predict biologic behavior of dysplasia and carcinoma in UC compared to allelic deletion studies alone. Further microdissection studies are necessary to evaluate the possibility of different carcinoma risk in patients with low percentage of p53 overexpression and associated LOH.

Original languageEnglish (US)
Pages (from-to)477-480
Number of pages4
JournalOncology reports
Volume5
Issue number2
DOIs
StatePublished - 1998
Externally publishedYes

Keywords

  • Loss of heterozygosity
  • Ulcerative colitis
  • p53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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