Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos

Debra Hickman, David J. Beebe, Sandra L. Rodriguez-Zas, Matthew B. Wheeler

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The ability to manipulate mouse pre-implantation embryos in vitro has become a valuable tool in many scientific disciplines. However, fewer embryos maintain viability following in vitro manipulation compared with embryos in vivo. It has been suggested that use of dynamic medium environments may improve viability by simulating in utero environment. The objective of the study reported here was to compare a microdrop in vitro culture system with a microdevice in vitro culture system containing a static and two dynamic medium environments (0.1 and 0.5 μl/h) for culturing of mouse pre-implantation embryos. Results indicated that the static medium environment, in silicon-glass microdevices, was not significantly different from the microdrop control environment in proportion of embryos developing from the two-cell to the blastocyst stage. However, the static microdevice environment produced significantly (P <0.05) more morulas than did that of the control group. Both of these treatment groups, under the presented conditions, consistently had significantly higher proportions of blastocysts (P <0.05) and morulas (P <0.05) and lower proportions of abnormal (P <0.05) and eight-cell embryos (P <0.05), compared with those of the high flow rate dynamic environment microdevice treatment groups. Studies exploring slower or pulsatile rates of medium delivery in a dynamic medium environment are indicated.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalComparative Medicine
Volume52
Issue number2
StatePublished - 2002
Externally publishedYes

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embryo (animal)
mice
Embryonic Structures
embryo implantation
Morula
morula
Blastocyst
blastocyst
in vitro culture
Silicon
viability
Flow rate
silicon
Glass
glass
cells
Control Groups
In Vitro Techniques

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos. / Hickman, Debra; Beebe, David J.; Rodriguez-Zas, Sandra L.; Wheeler, Matthew B.

In: Comparative Medicine, Vol. 52, No. 2, 2002, p. 122-126.

Research output: Contribution to journalArticle

Hickman, Debra ; Beebe, David J. ; Rodriguez-Zas, Sandra L. ; Wheeler, Matthew B. / Comparison of static and dynamic medium environments for culturing of pre-implantation mouse embryos. In: Comparative Medicine. 2002 ; Vol. 52, No. 2. pp. 122-126.
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