Comparison of the catalytic domains of collagenase-1 and stromelysin-1

Liang Yan Hu, Shao Min Tian, Qizhuang Ye, Kang Cheng Ruan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The catalytic domains of two matrix metalloproteinases - collagenase-1 and stromelysin-1 have been studied by means of fluorescence spectroscopy and high hydrostatic pressure. The hydrophobic fluorescence probe ANS could bind to stromelysin-1, with a dissociation constant of 26.3 μmol/L, but could not bind to collagenase-1, indicating that there exists a hydrophobic site on the surface of stromelysin-1. Further study suggests that the hydrophobic site may not be the catalytic site. The biological activity of catalytic domains of collagenase-1 and stromelysin-1 showed obvious difference under high pressure: the activity of collagenase-1 increased with elevating pressure, with an apparent activation volume of - 18. 9 ml/mol; however, the activity of stromelysin-1 did not change under high pressure. The results indicate that there are some obvious differences between the catalytic domain conformations of these two enzymes, though the crystal analysis indicated that they were very similar as reported before.

Original languageEnglish (US)
Pages (from-to)411-412
Number of pages2
JournalActa Biochimica et Biophysica Sinica
Volume32
Issue number4
StatePublished - 2000
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 3
Catalytic Domain
Pressure
Matrix Metalloproteinase 1
Hydrostatic Pressure
Fluorescence Spectrometry
Fluorescence spectroscopy
Hydrostatic pressure
Bioactivity
Matrix Metalloproteinases
Conformations
Fluorescence
Chemical activation
collagenase 1
Crystals
Enzymes

Keywords

  • ANS
  • Collagenase-1
  • High pressure
  • Matrix metalloproteinase
  • Stromelysin-1

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

Cite this

Comparison of the catalytic domains of collagenase-1 and stromelysin-1. / Hu, Liang Yan; Tian, Shao Min; Ye, Qizhuang; Ruan, Kang Cheng.

In: Acta Biochimica et Biophysica Sinica, Vol. 32, No. 4, 2000, p. 411-412.

Research output: Contribution to journalArticle

Hu, Liang Yan ; Tian, Shao Min ; Ye, Qizhuang ; Ruan, Kang Cheng. / Comparison of the catalytic domains of collagenase-1 and stromelysin-1. In: Acta Biochimica et Biophysica Sinica. 2000 ; Vol. 32, No. 4. pp. 411-412.
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