Comparison of the effects of 2-deoxyglucose and immobilization on secretion and synthesis rate of catecholamines in the adrenal gland

A microdialysis study in conscious rats

A. I. Kuzmin, V. M. Pogorelov, Dmitry Zaretsky, O. S. Medvedev, E. I. Chazov

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Using microdialysis, extracellular 3,4-dihydroxyphenylalanine (DOPA), noradrenaline (NA) and adrenaline (AD) concentrations in the adrenal gland were monitored in conscious rats during and after 60 min of immobilization (IMM) as well as after injection of 500 mg kg-1 2-deoxyglucose (2-DG). IMM produced a rapid and transient increase in secretion of AD (20-fold), NA (13-fold) and DOPA (3.6-fold). This was accompanied by an increase in blood pressure (+18 mmHg) and heart rate (+146 b.p.m.). Repeated exposure to IMM (daily 60 min, far 5 days) had no influence on either catecholamine secretion of haemodynamic profiles, indicating the lack of habituation to stressful conditions. Unlike IMM, the stress of 2-DG-induced central neuroglucopenia stimulated the release of AD without affecting NA secretion. AD levels peaked (5.1-fold increase) 40-60 min after 2-DG injection and then slowly declined. 2-DG induced no changes in blood pressure but reduced the heart rate (-48 b.p.m.). In separate experiments, steady-state dialysate DOPA levels, reached during continuous infusion of decarboxylase inhibitor NSD 1015 into adrenal gland tissue through the dialysis probe, served as an index of adrenomedullary tyrosine hydroxylase (TH) activity. IMM evoked a marked increase in TH activity (DOPA formation increased 2.7-fold), which remained elevated 60 min after the cessation of stress when AD and NA secretion had already fallen to baseline. After 2-DG, despite significant hormonal response, adrenal TH activity was unchanged. These results give clear evidence that IMM and 2-DG-induced neuroglucopenia may be considered as two different types of stressful stimuli.

Original languageEnglish (US)
Pages (from-to)147-155
Number of pages9
JournalActa Physiologica Scandinavica
Volume155
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

Microdialysis
Deoxyglucose
Adrenal Glands
Immobilization
Catecholamines
Dihydroxyphenylalanine
Epinephrine
Norepinephrine
Tyrosine 3-Monooxygenase
Heart Rate
Blood Pressure
Injections
Carboxy-Lyases
Dialysis Solutions
Dialysis
Hemodynamics

Keywords

  • Adrenal gland
  • Catecholamine secretion
  • Catecholamine synthesis rate
  • Haemodynamics
  • Microdialysis
  • Stress

ASJC Scopus subject areas

  • Physiology

Cite this

Comparison of the effects of 2-deoxyglucose and immobilization on secretion and synthesis rate of catecholamines in the adrenal gland : A microdialysis study in conscious rats. / Kuzmin, A. I.; Pogorelov, V. M.; Zaretsky, Dmitry; Medvedev, O. S.; Chazov, E. I.

In: Acta Physiologica Scandinavica, Vol. 155, No. 2, 1995, p. 147-155.

Research output: Contribution to journalArticle

@article{1d822dfe83ca4fdd9427c91a35492f71,
title = "Comparison of the effects of 2-deoxyglucose and immobilization on secretion and synthesis rate of catecholamines in the adrenal gland: A microdialysis study in conscious rats",
abstract = "Using microdialysis, extracellular 3,4-dihydroxyphenylalanine (DOPA), noradrenaline (NA) and adrenaline (AD) concentrations in the adrenal gland were monitored in conscious rats during and after 60 min of immobilization (IMM) as well as after injection of 500 mg kg-1 2-deoxyglucose (2-DG). IMM produced a rapid and transient increase in secretion of AD (20-fold), NA (13-fold) and DOPA (3.6-fold). This was accompanied by an increase in blood pressure (+18 mmHg) and heart rate (+146 b.p.m.). Repeated exposure to IMM (daily 60 min, far 5 days) had no influence on either catecholamine secretion of haemodynamic profiles, indicating the lack of habituation to stressful conditions. Unlike IMM, the stress of 2-DG-induced central neuroglucopenia stimulated the release of AD without affecting NA secretion. AD levels peaked (5.1-fold increase) 40-60 min after 2-DG injection and then slowly declined. 2-DG induced no changes in blood pressure but reduced the heart rate (-48 b.p.m.). In separate experiments, steady-state dialysate DOPA levels, reached during continuous infusion of decarboxylase inhibitor NSD 1015 into adrenal gland tissue through the dialysis probe, served as an index of adrenomedullary tyrosine hydroxylase (TH) activity. IMM evoked a marked increase in TH activity (DOPA formation increased 2.7-fold), which remained elevated 60 min after the cessation of stress when AD and NA secretion had already fallen to baseline. After 2-DG, despite significant hormonal response, adrenal TH activity was unchanged. These results give clear evidence that IMM and 2-DG-induced neuroglucopenia may be considered as two different types of stressful stimuli.",
keywords = "Adrenal gland, Catecholamine secretion, Catecholamine synthesis rate, Haemodynamics, Microdialysis, Stress",
author = "Kuzmin, {A. I.} and Pogorelov, {V. M.} and Dmitry Zaretsky and Medvedev, {O. S.} and Chazov, {E. I.}",
year = "1995",
language = "English (US)",
volume = "155",
pages = "147--155",
journal = "Acta Physiologica Scandinavica",
issn = "0370-839X",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Comparison of the effects of 2-deoxyglucose and immobilization on secretion and synthesis rate of catecholamines in the adrenal gland

T2 - A microdialysis study in conscious rats

AU - Kuzmin, A. I.

AU - Pogorelov, V. M.

AU - Zaretsky, Dmitry

AU - Medvedev, O. S.

AU - Chazov, E. I.

PY - 1995

Y1 - 1995

N2 - Using microdialysis, extracellular 3,4-dihydroxyphenylalanine (DOPA), noradrenaline (NA) and adrenaline (AD) concentrations in the adrenal gland were monitored in conscious rats during and after 60 min of immobilization (IMM) as well as after injection of 500 mg kg-1 2-deoxyglucose (2-DG). IMM produced a rapid and transient increase in secretion of AD (20-fold), NA (13-fold) and DOPA (3.6-fold). This was accompanied by an increase in blood pressure (+18 mmHg) and heart rate (+146 b.p.m.). Repeated exposure to IMM (daily 60 min, far 5 days) had no influence on either catecholamine secretion of haemodynamic profiles, indicating the lack of habituation to stressful conditions. Unlike IMM, the stress of 2-DG-induced central neuroglucopenia stimulated the release of AD without affecting NA secretion. AD levels peaked (5.1-fold increase) 40-60 min after 2-DG injection and then slowly declined. 2-DG induced no changes in blood pressure but reduced the heart rate (-48 b.p.m.). In separate experiments, steady-state dialysate DOPA levels, reached during continuous infusion of decarboxylase inhibitor NSD 1015 into adrenal gland tissue through the dialysis probe, served as an index of adrenomedullary tyrosine hydroxylase (TH) activity. IMM evoked a marked increase in TH activity (DOPA formation increased 2.7-fold), which remained elevated 60 min after the cessation of stress when AD and NA secretion had already fallen to baseline. After 2-DG, despite significant hormonal response, adrenal TH activity was unchanged. These results give clear evidence that IMM and 2-DG-induced neuroglucopenia may be considered as two different types of stressful stimuli.

AB - Using microdialysis, extracellular 3,4-dihydroxyphenylalanine (DOPA), noradrenaline (NA) and adrenaline (AD) concentrations in the adrenal gland were monitored in conscious rats during and after 60 min of immobilization (IMM) as well as after injection of 500 mg kg-1 2-deoxyglucose (2-DG). IMM produced a rapid and transient increase in secretion of AD (20-fold), NA (13-fold) and DOPA (3.6-fold). This was accompanied by an increase in blood pressure (+18 mmHg) and heart rate (+146 b.p.m.). Repeated exposure to IMM (daily 60 min, far 5 days) had no influence on either catecholamine secretion of haemodynamic profiles, indicating the lack of habituation to stressful conditions. Unlike IMM, the stress of 2-DG-induced central neuroglucopenia stimulated the release of AD without affecting NA secretion. AD levels peaked (5.1-fold increase) 40-60 min after 2-DG injection and then slowly declined. 2-DG induced no changes in blood pressure but reduced the heart rate (-48 b.p.m.). In separate experiments, steady-state dialysate DOPA levels, reached during continuous infusion of decarboxylase inhibitor NSD 1015 into adrenal gland tissue through the dialysis probe, served as an index of adrenomedullary tyrosine hydroxylase (TH) activity. IMM evoked a marked increase in TH activity (DOPA formation increased 2.7-fold), which remained elevated 60 min after the cessation of stress when AD and NA secretion had already fallen to baseline. After 2-DG, despite significant hormonal response, adrenal TH activity was unchanged. These results give clear evidence that IMM and 2-DG-induced neuroglucopenia may be considered as two different types of stressful stimuli.

KW - Adrenal gland

KW - Catecholamine secretion

KW - Catecholamine synthesis rate

KW - Haemodynamics

KW - Microdialysis

KW - Stress

UR - http://www.scopus.com/inward/record.url?scp=0028826938&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028826938&partnerID=8YFLogxK

M3 - Article

VL - 155

SP - 147

EP - 155

JO - Acta Physiologica Scandinavica

JF - Acta Physiologica Scandinavica

SN - 0370-839X

IS - 2

ER -