Comparison of the enzymatic and immunological properties of direct factor X activator isolated from human and swine amnion-chorion membranes and from rat prostate cancer

Katarzyna Kaplińska, Malgorzata Kamocka, Elliot Rosen, Wojciech P. Mielicki

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Abstract

The effect of iodoacetamide (IAA), copper (Cu2+) and manganese (Mn2+) ions on the activity of direct factor X-activator (procoagulant) isolated from human and swine amnion-chorion membranes and from rat prostate carcinoma has been analysed. IAA (1 mM) inhibited all the procoagulants in a similar degree. 1 mM Cu2+ significantly reduced the activity of human and swine but not of rat procoagulant. 1 mM Mn 2+ ions enhanced the activity of human procoagulant, they did not affect the activity of rat procoagulant and reduced the activity of swine procoagulant. The immunological similarity of all the procoagulants was evaluated by an ELISA using polyclonal rabbit anti-human cancer procoagulant (CP) antibody. Human and swine procoagulants were detected by the antibody in the similar manner. However, factor X-activator from rat carcinoma was recognized by the antibody significantly less effectively. The results suggest that there are differences in enzymatic as well as in antigenic properties between procoagulants isolated from different species.

Original languageEnglish
Pages (from-to)331-338
Number of pages8
JournalActa Haematologica Polonica
Volume38
Issue number3
StatePublished - 2007

Fingerprint

cancer procoagulant
Chorion
Amnion
Prostatic Neoplasms
Swine
Iodoacetamide
Membranes
Human Activities
Antibodies
Ions
Carcinoma
Manganese
Copper
Prostate
Enzyme-Linked Immunosorbent Assay
Rabbits

Keywords

  • Cancer procoagulant
  • Divalent ions
  • Enzymatic activity
  • Iodoacetamide

ASJC Scopus subject areas

  • Hematology

Cite this

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abstract = "The effect of iodoacetamide (IAA), copper (Cu2+) and manganese (Mn2+) ions on the activity of direct factor X-activator (procoagulant) isolated from human and swine amnion-chorion membranes and from rat prostate carcinoma has been analysed. IAA (1 mM) inhibited all the procoagulants in a similar degree. 1 mM Cu2+ significantly reduced the activity of human and swine but not of rat procoagulant. 1 mM Mn 2+ ions enhanced the activity of human procoagulant, they did not affect the activity of rat procoagulant and reduced the activity of swine procoagulant. The immunological similarity of all the procoagulants was evaluated by an ELISA using polyclonal rabbit anti-human cancer procoagulant (CP) antibody. Human and swine procoagulants were detected by the antibody in the similar manner. However, factor X-activator from rat carcinoma was recognized by the antibody significantly less effectively. The results suggest that there are differences in enzymatic as well as in antigenic properties between procoagulants isolated from different species.",
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T1 - Comparison of the enzymatic and immunological properties of direct factor X activator isolated from human and swine amnion-chorion membranes and from rat prostate cancer

AU - Kaplińska, Katarzyna

AU - Kamocka, Malgorzata

AU - Rosen, Elliot

AU - Mielicki, Wojciech P.

PY - 2007

Y1 - 2007

N2 - The effect of iodoacetamide (IAA), copper (Cu2+) and manganese (Mn2+) ions on the activity of direct factor X-activator (procoagulant) isolated from human and swine amnion-chorion membranes and from rat prostate carcinoma has been analysed. IAA (1 mM) inhibited all the procoagulants in a similar degree. 1 mM Cu2+ significantly reduced the activity of human and swine but not of rat procoagulant. 1 mM Mn 2+ ions enhanced the activity of human procoagulant, they did not affect the activity of rat procoagulant and reduced the activity of swine procoagulant. The immunological similarity of all the procoagulants was evaluated by an ELISA using polyclonal rabbit anti-human cancer procoagulant (CP) antibody. Human and swine procoagulants were detected by the antibody in the similar manner. However, factor X-activator from rat carcinoma was recognized by the antibody significantly less effectively. The results suggest that there are differences in enzymatic as well as in antigenic properties between procoagulants isolated from different species.

AB - The effect of iodoacetamide (IAA), copper (Cu2+) and manganese (Mn2+) ions on the activity of direct factor X-activator (procoagulant) isolated from human and swine amnion-chorion membranes and from rat prostate carcinoma has been analysed. IAA (1 mM) inhibited all the procoagulants in a similar degree. 1 mM Cu2+ significantly reduced the activity of human and swine but not of rat procoagulant. 1 mM Mn 2+ ions enhanced the activity of human procoagulant, they did not affect the activity of rat procoagulant and reduced the activity of swine procoagulant. The immunological similarity of all the procoagulants was evaluated by an ELISA using polyclonal rabbit anti-human cancer procoagulant (CP) antibody. Human and swine procoagulants were detected by the antibody in the similar manner. However, factor X-activator from rat carcinoma was recognized by the antibody significantly less effectively. The results suggest that there are differences in enzymatic as well as in antigenic properties between procoagulants isolated from different species.

KW - Cancer procoagulant

KW - Divalent ions

KW - Enzymatic activity

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