Comparison of the enzymatic and immunological properties of direct factor X activator isolated from human and swine amnion-chorion membranes and from rat prostate cancer

Katarzyna Kaplińska, Małgorzata Kamocka, Elliot D. Rosen, Wojciech P. Mielicki

Research output: Contribution to journalArticle

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Abstract

The effect of iodoacetamide (IAA), copper (Cu2+) and manganese (Mn2+) ions on the activity of direct factor X-activator (procoagulant) isolated from human and swine amnion-chorion membranes and from rat prostate carcinoma has been analysed. IAA (1 mM) inhibited all the procoagulants in a similar degree. 1 mM Cu2+ significantly reduced the activity of human and swine but not of rat procoagulant. 1 mM Mn 2+ ions enhanced the activity of human procoagulant, they did not affect the activity of rat procoagulant and reduced the activity of swine procoagulant. The immunological similarity of all the procoagulants was evaluated by an ELISA using polyclonal rabbit anti-human cancer procoagulant (CP) antibody. Human and swine procoagulants were detected by the antibody in the similar manner. However, factor X-activator from rat carcinoma was recognized by the antibody significantly less effectively. The results suggest that there are differences in enzymatic as well as in antigenic properties between procoagulants isolated from different species.

Original languageEnglish (US)
Pages (from-to)331-338
Number of pages8
JournalActa Haematologica Polonica
Volume38
Issue number3
StatePublished - Jan 1 2007

Keywords

  • Cancer procoagulant
  • Divalent ions
  • Enzymatic activity
  • Iodoacetamide

ASJC Scopus subject areas

  • Hematology

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