Comparison of the respiratory microbiome in healthy nonsmokers and smokers

Alison Morris, James M. Beck, Patrick D. Schloss, Thomas B. Campbell, Kristina Crothers, Jeffrey L. Curtis, Sonia C. Flores, Andrew P. Fontenot, Elodie Ghedin, Laurence Huang, Kathleen Jablonski, Eric Kleerup, Susan V. Lynch, Erica Sodergren, Homer Twigg, Vincent B. Young, Christine M. Bassis, Arvind Venkataraman, Thomas M. Schmidt, George M. Weinstock

Research output: Contribution to journalArticle

309 Citations (Scopus)

Abstract

Rationale: Results from 16S rDNA-encoding gene sequence-based, culture-independent techniques have led to conflicting conclusions about the composition of the lower respiratory tract microbiome. Objectives: To compare the microbiome of the upper and lower respiratory tract in healthy HIV-uninfected nonsmokers and smokers in a multicenter cohort. Methods: Participants were nonsmokers and smokers without significant comorbidities. Oral washes and bronchoscopic alveolar lavages were collected in a standardized manner. Sequence analysis of bacterial 16S rRNA-encoding genes was performed, and the neutral model in community ecology was used to identify bacteria that were the most plausible members of a lung microbiome. Measurements and Main Results: Sixty-four participants were enrolled. Most bacteria identified in the lung were also in the mouth, but specific bacteria such as Enterobacteriaceae, Haemophilus, Methylobacterium, and Ralstonia species were disproportionally represented in the lungs compared with values predicted by the neutralmodel. Tropheryma was also in the lung, but not the mouth. Mouth communities differed between nonsmokers and smokers in species such as Porphyromonas, Neisseria, and Gemella, but lung bacterial populations did not. Conclusions: This study is the largest to examine composition of the lower respiratory tract microbiome in healthy individuals and the first to use the neutral model to compare the lung to the mouth. Specificbacteria appear insignificantly higher abundance in the lungs than would be expected if they originated from the mouth, demonstrating that the lung microbiome does not derive entirely from the mouth. The mouth microbiome differs in nonsmokers and smokers, but lung communities were not significantly altered by smoking.

Original languageEnglish (US)
Pages (from-to)1067-1075
Number of pages9
JournalAmerican journal of respiratory and critical care medicine
Volume187
Issue number10
DOIs
StatePublished - May 15 2013

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Microbiota
Mouth
Lung
Respiratory System
Bacteria
Gemella
Tropheryma
Ralstonia
Porphyromonas
Methylobacterium
Haemophilus
Neisseria
Culture Techniques
Therapeutic Irrigation
Enterobacteriaceae
Ribosomal DNA
Ecology
rRNA Genes
Sequence Analysis
Comorbidity

Keywords

  • Bronchoscopy
  • Lung
  • Metagenome
  • Microbiome
  • Smoking

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Morris, A., Beck, J. M., Schloss, P. D., Campbell, T. B., Crothers, K., Curtis, J. L., ... Weinstock, G. M. (2013). Comparison of the respiratory microbiome in healthy nonsmokers and smokers. American journal of respiratory and critical care medicine, 187(10), 1067-1075. https://doi.org/10.1164/rccm.201210-1913OC

Comparison of the respiratory microbiome in healthy nonsmokers and smokers. / Morris, Alison; Beck, James M.; Schloss, Patrick D.; Campbell, Thomas B.; Crothers, Kristina; Curtis, Jeffrey L.; Flores, Sonia C.; Fontenot, Andrew P.; Ghedin, Elodie; Huang, Laurence; Jablonski, Kathleen; Kleerup, Eric; Lynch, Susan V.; Sodergren, Erica; Twigg, Homer; Young, Vincent B.; Bassis, Christine M.; Venkataraman, Arvind; Schmidt, Thomas M.; Weinstock, George M.

In: American journal of respiratory and critical care medicine, Vol. 187, No. 10, 15.05.2013, p. 1067-1075.

Research output: Contribution to journalArticle

Morris, A, Beck, JM, Schloss, PD, Campbell, TB, Crothers, K, Curtis, JL, Flores, SC, Fontenot, AP, Ghedin, E, Huang, L, Jablonski, K, Kleerup, E, Lynch, SV, Sodergren, E, Twigg, H, Young, VB, Bassis, CM, Venkataraman, A, Schmidt, TM & Weinstock, GM 2013, 'Comparison of the respiratory microbiome in healthy nonsmokers and smokers', American journal of respiratory and critical care medicine, vol. 187, no. 10, pp. 1067-1075. https://doi.org/10.1164/rccm.201210-1913OC
Morris, Alison ; Beck, James M. ; Schloss, Patrick D. ; Campbell, Thomas B. ; Crothers, Kristina ; Curtis, Jeffrey L. ; Flores, Sonia C. ; Fontenot, Andrew P. ; Ghedin, Elodie ; Huang, Laurence ; Jablonski, Kathleen ; Kleerup, Eric ; Lynch, Susan V. ; Sodergren, Erica ; Twigg, Homer ; Young, Vincent B. ; Bassis, Christine M. ; Venkataraman, Arvind ; Schmidt, Thomas M. ; Weinstock, George M. / Comparison of the respiratory microbiome in healthy nonsmokers and smokers. In: American journal of respiratory and critical care medicine. 2013 ; Vol. 187, No. 10. pp. 1067-1075.
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