Competitive interaction of cyclosporins with the Vinca alkaloid-binding site of P-glycoprotein in multidrug-resistant cells

I. Tamai, A. R. Safa

Research output: Contribution to journalArticle

161 Scopus citations

Abstract

The mechanism of reversal of resistance to Vinca alkaloids by cyclosporins is unclear. We investigated the molecular mechanism of reversal of Vinca alkaloid resistance by cyclosporin A (CsA) and its nonimmunosuppressive analog O-acetyl C91 CsA (SDZ 33-243) in multidrug resistant DC-3F/VCRd-5L Chinese hamster cells. CsA at 3 μM increased vincristine (VCR) sensitivity and almost totally reversed VCR resistance. SDZ 33-243 at 1 μM reduced the IC50 for VCR in resistant cells from 62.0 to 0.00062 μM. CsA and SDZ 33-243 at 10 μM increased [3H]vinblastine (VBL) accumulation in DC-3F/VCRd-5L cells by 27- and 22-fold, respectively. At 10 μM, these compounds also increased [3H]VCR accumulation by 3.5- and 4.0-fold, respectively. [3H]VCR uptake by membrane vesicles from DC-3F/VCRd-5L cells showed high and low affinity components with Michaelis-Menten kinetics, and apparent K(m) values were 0.140 ± 0.0523 and 24.8 ± 6.67 μM, respectively. Kinetic analysis of [3H]VCR uptake in membrane vesicles in the presence of 0.2 μM CsA revealed that CsA competitively inhibited the high affinity [3H]VCR uptake with an apparent inhibition constant (K(i)) of 0.126 ± 0.0173 μM. In addition, CsA and SDZ 33-243 inhibited VBL photoaffinity labeling of P-glycoprotein in a dose-dependent manner, with half-maximum inhibition at 0.5 and 0.4 μM, respectively, compared with that of VBL at 0.6 μM. These data confirm that cyclosporins modulate Vinca alkaloid resistance at least partially through interaction with P-glycoprotein.

Original languageEnglish (US)
Pages (from-to)16509-16513
Number of pages5
JournalJournal of Biological Chemistry
Volume265
Issue number27
StatePublished - Oct 22 1990
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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