Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes

William M. Baldwin, Milagros Samaniego-Picota, Edward K. Kasper, Adam M. Clark, Magdalena Czader, Charles Rohde, Andrea A. Zachary, Fred Sanfilippo, Ralph H. Hruban

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Background. Prolonged warm or cold ischemia is associated with poor survival of cardiac transplants, and ischemic changes in early posttransplantation endomyocardial biopsies correlate with the later development of chronic rejection. In animal models, tissue ischemia has been shown to activate complement. Methods. To determine whether ischemic changes in endomyocardial biopsies were associated with complement deposition, biopsies obtained 1-3 weeks after transplantation from 33 patients were evaluated immunohistologically for C4d and C3d deposition as well as for IgM, IgG, and IgA. The histological changes associated with ischemic injury were scored independently, using previously reported criteria without knowledge of the immunohistochemical results. Results. Diffuse capillary and pericapillary deposition of C4d or C3d were detected in endomyocardial biopsies of 14 of the 33 patients. The majority of biopsies (79%) with C4d or C3d deposits had histological evidence of ischemic injury, including eight of the nine biopsies containing both C4d and C3d deposition. In contrast, only 8 of 18 (45%) of the biopsies without C4d or C3d deposition had ischemic injury. Only trace amounts of IgM and no IgG or IgA were demonstrable in the biopsies. Only 2 of the 14 biopsies with C4d or C3d deposition had evidence of moderate acute rejection, whereas 5 of the 18 biopsies without C4d or C3d deposition had moderate acute rejection. However, C4d and C3d deposition did correlate with repeated acute rejection episodes on subsequent biopsies. Conclusions. Thus, ischemic changes are associated with the activation of complement. Complement activation may in turn promote tissue injury and provide a potential target for future treatment.

Original languageEnglish (US)
Pages (from-to)894-900
Number of pages7
JournalTransplantation
Volume68
Issue number6
DOIs
StatePublished - Sep 27 1999
Externally publishedYes

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Transplants
Biopsy
Wounds and Injuries
Complement Activation
Immunoglobulin A
Immunoglobulin M
Immunoglobulin G
Cold Ischemia
Warm Ischemia
Ischemia
Animal Models
Transplantation

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes. / Baldwin, William M.; Samaniego-Picota, Milagros; Kasper, Edward K.; Clark, Adam M.; Czader, Magdalena; Rohde, Charles; Zachary, Andrea A.; Sanfilippo, Fred; Hruban, Ralph H.

In: Transplantation, Vol. 68, No. 6, 27.09.1999, p. 894-900.

Research output: Contribution to journalArticle

Baldwin, WM, Samaniego-Picota, M, Kasper, EK, Clark, AM, Czader, M, Rohde, C, Zachary, AA, Sanfilippo, F & Hruban, RH 1999, 'Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes', Transplantation, vol. 68, no. 6, pp. 894-900. https://doi.org/10.1097/00007890-199909270-00024
Baldwin, William M. ; Samaniego-Picota, Milagros ; Kasper, Edward K. ; Clark, Adam M. ; Czader, Magdalena ; Rohde, Charles ; Zachary, Andrea A. ; Sanfilippo, Fred ; Hruban, Ralph H. / Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes. In: Transplantation. 1999 ; Vol. 68, No. 6. pp. 894-900.
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abstract = "Background. Prolonged warm or cold ischemia is associated with poor survival of cardiac transplants, and ischemic changes in early posttransplantation endomyocardial biopsies correlate with the later development of chronic rejection. In animal models, tissue ischemia has been shown to activate complement. Methods. To determine whether ischemic changes in endomyocardial biopsies were associated with complement deposition, biopsies obtained 1-3 weeks after transplantation from 33 patients were evaluated immunohistologically for C4d and C3d deposition as well as for IgM, IgG, and IgA. The histological changes associated with ischemic injury were scored independently, using previously reported criteria without knowledge of the immunohistochemical results. Results. Diffuse capillary and pericapillary deposition of C4d or C3d were detected in endomyocardial biopsies of 14 of the 33 patients. The majority of biopsies (79{\%}) with C4d or C3d deposits had histological evidence of ischemic injury, including eight of the nine biopsies containing both C4d and C3d deposition. In contrast, only 8 of 18 (45{\%}) of the biopsies without C4d or C3d deposition had ischemic injury. Only trace amounts of IgM and no IgG or IgA were demonstrable in the biopsies. Only 2 of the 14 biopsies with C4d or C3d deposition had evidence of moderate acute rejection, whereas 5 of the 18 biopsies without C4d or C3d deposition had moderate acute rejection. However, C4d and C3d deposition did correlate with repeated acute rejection episodes on subsequent biopsies. Conclusions. Thus, ischemic changes are associated with the activation of complement. Complement activation may in turn promote tissue injury and provide a potential target for future treatment.",
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T1 - Complement deposition in early cardiac transplant biopsies is associated with ischemic injury and subsequent rejection episodes

AU - Baldwin, William M.

AU - Samaniego-Picota, Milagros

AU - Kasper, Edward K.

AU - Clark, Adam M.

AU - Czader, Magdalena

AU - Rohde, Charles

AU - Zachary, Andrea A.

AU - Sanfilippo, Fred

AU - Hruban, Ralph H.

PY - 1999/9/27

Y1 - 1999/9/27

N2 - Background. Prolonged warm or cold ischemia is associated with poor survival of cardiac transplants, and ischemic changes in early posttransplantation endomyocardial biopsies correlate with the later development of chronic rejection. In animal models, tissue ischemia has been shown to activate complement. Methods. To determine whether ischemic changes in endomyocardial biopsies were associated with complement deposition, biopsies obtained 1-3 weeks after transplantation from 33 patients were evaluated immunohistologically for C4d and C3d deposition as well as for IgM, IgG, and IgA. The histological changes associated with ischemic injury were scored independently, using previously reported criteria without knowledge of the immunohistochemical results. Results. Diffuse capillary and pericapillary deposition of C4d or C3d were detected in endomyocardial biopsies of 14 of the 33 patients. The majority of biopsies (79%) with C4d or C3d deposits had histological evidence of ischemic injury, including eight of the nine biopsies containing both C4d and C3d deposition. In contrast, only 8 of 18 (45%) of the biopsies without C4d or C3d deposition had ischemic injury. Only trace amounts of IgM and no IgG or IgA were demonstrable in the biopsies. Only 2 of the 14 biopsies with C4d or C3d deposition had evidence of moderate acute rejection, whereas 5 of the 18 biopsies without C4d or C3d deposition had moderate acute rejection. However, C4d and C3d deposition did correlate with repeated acute rejection episodes on subsequent biopsies. Conclusions. Thus, ischemic changes are associated with the activation of complement. Complement activation may in turn promote tissue injury and provide a potential target for future treatment.

AB - Background. Prolonged warm or cold ischemia is associated with poor survival of cardiac transplants, and ischemic changes in early posttransplantation endomyocardial biopsies correlate with the later development of chronic rejection. In animal models, tissue ischemia has been shown to activate complement. Methods. To determine whether ischemic changes in endomyocardial biopsies were associated with complement deposition, biopsies obtained 1-3 weeks after transplantation from 33 patients were evaluated immunohistologically for C4d and C3d deposition as well as for IgM, IgG, and IgA. The histological changes associated with ischemic injury were scored independently, using previously reported criteria without knowledge of the immunohistochemical results. Results. Diffuse capillary and pericapillary deposition of C4d or C3d were detected in endomyocardial biopsies of 14 of the 33 patients. The majority of biopsies (79%) with C4d or C3d deposits had histological evidence of ischemic injury, including eight of the nine biopsies containing both C4d and C3d deposition. In contrast, only 8 of 18 (45%) of the biopsies without C4d or C3d deposition had ischemic injury. Only trace amounts of IgM and no IgG or IgA were demonstrable in the biopsies. Only 2 of the 14 biopsies with C4d or C3d deposition had evidence of moderate acute rejection, whereas 5 of the 18 biopsies without C4d or C3d deposition had moderate acute rejection. However, C4d and C3d deposition did correlate with repeated acute rejection episodes on subsequent biopsies. Conclusions. Thus, ischemic changes are associated with the activation of complement. Complement activation may in turn promote tissue injury and provide a potential target for future treatment.

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