Composition of the extracellular matrix of the peritoneum

Craig A. Witz, Iris A. Montoya-Rodriguez, Sook Cho, Victoria E. Centonze, Lynda Bonewald, Robert S. Schenken

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

Objective: To localize the extracellular matrix proteins collagen I, collagen IV, fibronectin, and laminin in the peritoneal membrane. Study Design: Peritoneal biopsies (n = 13) from the anterior abdominal wall and the uterine serosa (n = 3) were incubated with antibodies to collagen IV, laminin, collagen I, and fibronectin. Specimens were examined using light and confocal laser scanning microscopy. Results: All of the extracellular matrix (ECM) proteins were present immediately under the mesothelium. Collagen (Col) IV and laminin (LM) were seen in the smooth muscle of microvascular structures, in the subendothelial basement membrane, and were present in a fascicular pattern in the peritoneal stroma. Collagen I was distributed diffusely in the peritoneal stroma. Fibronectin was also present in the subendothelial basement membrane. Conclusions: The resolution of the confocal microscope allowed for localization of extracellular matrix proteins in relation to the mesothelium. The presence of collagen IV, laminin, collagen I, and fibronectin under the mesothelium suggests that cells invading the peritoneum must have the ability to degrade and remodel this matrix.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalJournal of the Society for Gynecologic Investigation
Volume8
Issue number5
DOIs
StatePublished - Sep 10 2001
Externally publishedYes

Fingerprint

Peritoneum
Extracellular Matrix
Collagen
Laminin
Fibronectins
Extracellular Matrix Proteins
Epithelium
Basement Membrane
Serous Membrane
Abdominal Wall
Confocal Microscopy
Smooth Muscle
Biopsy
Light
Membranes
Antibodies

Keywords

  • Cancer metastasis
  • Collagen
  • Confocal microscopy
  • Endometriosis
  • Fibronectin
  • Laminin
  • Peritoneum

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Composition of the extracellular matrix of the peritoneum. / Witz, Craig A.; Montoya-Rodriguez, Iris A.; Cho, Sook; Centonze, Victoria E.; Bonewald, Lynda; Schenken, Robert S.

In: Journal of the Society for Gynecologic Investigation, Vol. 8, No. 5, 10.09.2001, p. 299-304.

Research output: Contribution to journalArticle

Witz, Craig A. ; Montoya-Rodriguez, Iris A. ; Cho, Sook ; Centonze, Victoria E. ; Bonewald, Lynda ; Schenken, Robert S. / Composition of the extracellular matrix of the peritoneum. In: Journal of the Society for Gynecologic Investigation. 2001 ; Vol. 8, No. 5. pp. 299-304.
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