Concentration dependence of vitamin C in combinations with vitamin e and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells

Małgorzata Rózanowska, Linda Bakker, Michael E. Boulton, Bartosz Rózanowski

Research output: Contribution to journalArticle

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Abstract

The purpose of this study was to determine the effects of increasing concentration of ascorbate alone and in combinations with α-tocopherol and zeaxanthin on phototoxicity to the retinal pigment epithelium. ARPE-19 cells were exposed to rose bengal and visible light in the presence and absence of antioxidants. Toxicity was quantified by an assay of cell-reductive activity. A 20 min exposure to visible light and photosensitizer decreased cell viability to ca 42%. Lipophilic antioxidants increased viabilities to ca 70%, 61% and 75% for α-tocopherol, zeaxanthin and their combination, respectively. Cell viabilities were ca 70%, 56% and 5% after exposures in the presence of 0.35, 0.7 and 1.4 mm ascorbate, respectively. A 45 min exposure increased cell death to ca 74% and >95% in the absence and presence of ascorbate, respectively. In the presence of ascorbate, zeaxanthin did not significantly affect phototoxicity. α-Tocopherol and its combination with zeaxanthin enhanced protective effects of ascorbate, but did not prevent from ascorbate-mediated deleterious effects. In conclusion, there is a narrow range of concentrations and exposure times where ascorbate exerts photoprotective effects, exceeding which leads to ascorbate-mediated increase in photocytotoxicity. Vitamin E and its combination with zeaxanthin can enhance protective effects of ascorbate, but do not ameliorate its deleterious effects.

Original languageEnglish (US)
Pages (from-to)1408-1417
Number of pages10
JournalPhotochemistry and Photobiology
Volume88
Issue number6
DOIs
StatePublished - Nov 2012
Externally publishedYes

Fingerprint

vitamins
Retinal Pigments
ascorbic acid
pigments
Vitamins
toxicity
Ascorbic Acid
Toxicity
Epithelial Cells
tocopherol
Tocopherols
Light
Phototoxic Dermatitis
viability
Cell Survival
antioxidants
Antioxidants
Cells
Rose Bengal
Photosensitizing Agents

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Biochemistry
  • Medicine(all)

Cite this

Concentration dependence of vitamin C in combinations with vitamin e and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells. / Rózanowska, Małgorzata; Bakker, Linda; Boulton, Michael E.; Rózanowski, Bartosz.

In: Photochemistry and Photobiology, Vol. 88, No. 6, 11.2012, p. 1408-1417.

Research output: Contribution to journalArticle

Rózanowska, Małgorzata ; Bakker, Linda ; Boulton, Michael E. ; Rózanowski, Bartosz. / Concentration dependence of vitamin C in combinations with vitamin e and zeaxanthin on light-induced toxicity to retinal pigment epithelial cells. In: Photochemistry and Photobiology. 2012 ; Vol. 88, No. 6. pp. 1408-1417.
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